STUDIES ON THE MECHANISM OF ANTIDEPRESSANTS USING CLONAL CELL LINES (II)

将C_6胶质瘤细胞在含去甲丙咪嗪（DESIPRAMINE，HAL）或氟哌啶醇（HAL）的培养基中培养4天。10 μ M剂量的HAL不仅能减少异丙肾上腺素刺激的细胞中cAMP的积累，而且也能减少cAMP的积累。10 μ M的ATP降低了细胞中的总细胞蛋白和ATP水平。胞内水平的α-淀粉酶浓度比胞外水平高约50倍。10 μ M的β-内酰胺酶在培养液中可能由于蓄积而对C_6细胞产生毒性作用。因此，为了研究C_6细胞的作用机制，其在培养基中的浓度应小于1 μ M。C_6细胞长期暴露于0.1-1 μ M的Na_2SO_4，对细胞内总蛋白和ATP水平均无影响，但不减少cAMP的积累。

C_6 gliom cells were cultured in the medium containing desipramine(DMI) or haloperidol(HAL) for 4 days. Not only DMI but also HAL, in a dose of 10 muM, reduced the accumulation of cyclic AMP (cAMP) in the cells stimulated by isoproterenol. 10 muM DMI reduced both total cell protein and ATP level in the cells. Intracellular level of DMI was approximately 50-fold more concentrated than the extracellular level. It is likely that 10 muM of DMI in the medium induce toxic effect on C_6 cells due to the accumulation into the cells. Therefore, to investigate the mechanism of action of DMI using C_6 cells, its concentration in the medium should be less than 1 muM. Chronic exposure of C_6 cells to 0.1-1 muM DMI, in which doses neither total cell protein nor ATP level in the cells was affected, did not decrease cAMP accumulation.