Teratogenicity Caused by Anesthetic Gas and Changes Depending on O_2 Concentration.

麻醉气体引起的致畸性及其随 O_2 浓度的变化。

• 批准号: 63570731
• 负责人: YAMADA Morimasa
• 金额: $ 1.28万
• 依托单位: Fujita Health University School Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570731/
• 关键词: cleft lip and palate; anesthetics; Teratogenicity; A; J strain mice; 催奇形性; A@J系マウス; 麻酔ガス被爆; 奇形発現域値の低い集団における麻酔薬の安全性に関する実験的研究; 口唇口蓋裂; 薬物による催奇形形成; 口唇口蓋裂自然発生マウス

We were examined the teratogenicity under the detrimental condition being exposed to the anesthetic gas in organogenesis period. Prior to this experiment, it was reported that cleft palate occurred, when uniformly exposed to 0.5% halothane for 8 hours during the organogenesis period. The animals used in this study were ddy A/J strain mice. The animals in the 11th day of gestation were exposed to 0.5-1.0% enflurane for 8 hours under three different conditions (O_2 concentration : 21%, 60% and 100%). Cesarean section was performed on the 18th day of gestation and the fetuses were examined for the presence of abnormalities.From the results of this experiment,1) When two strains of mice were exposed to 1.0% enflurane at 2 liters / min., there was none observed with cleft palate in the ddy strain mice, but observed a high rate of 14.2% in the A/J strain mice.2) Halothane and enflurane caused the occurrence of cleft palate in the A/J strain mice.3) The rate of occurrence of enflurane was lower than that of halothane under this condition.4) We suggested that the decreasing occurrence of cleft palate (in the 60% O_2 and 100% O_2 groups) in the A/J strain mice was possible to prevent a certain extent by increasing the concentration of oxygen in the anesthetic gas.5) We compared the teratogenicity of halothance, enflurane, seroflurane.

在器官发生期暴露于麻醉性气体的有害条件下，检测其致畸作用。在此实验之前，有报道称，在器官发生期间，当均匀暴露于0.5%氟烷8小时时，会发生腭裂。实验动物为ddy A/J系小鼠。将妊娠第11天的动物置于0.5~1.0%的安氟醚中，分别在3种不同的氧气浓度(氧气浓度：21%、60%和100%)下暴露8小时。在妊娠第18天进行剖宫产，并对胎儿进行异常检查。从本实验的结果来看，1)当两个品系的小鼠以2升/分钟的速度暴露于1.0%的安氟醚时，ddy品系的小鼠没有观察到腭裂，但在A/J品系小鼠中观察到高达14.2%的发生率。2)氟烷和安氟醚在A/J品系小鼠中引起腭裂的发生。3)在此条件下，安氟醚的发生率低于氟烷。4)我们认为减少A/J品系小鼠腭裂的发生(在60%O_2和100%O_2组中)可能是通过增加麻醉气体中的氧气浓度来预防的。5)比较了氟烷、安氟烷、丙氟醚的致畸作用。