Immunologic、 Virologic、 and Bacteriologec Studies of Tonsillar Focal Infections and Their Clinical Applications

扁桃体局灶性感染的免疫学、病毒学和细菌学研究及其临床应用

• 批准号: 63570814
• 负责人: YAMANAKA Noboru
• 金额: $ 1.22万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570814/
• 关键词: Tonsillar Focal Infections; Pustulosis Palmaris Et Plantaris; Sterno-Cost-Clavicular Hyperostosis; Soluble Immune Complexes; Anti-Keration Antibodies; Autoantibodies; Image Analysis; 掌蹠膿庖症; EBウイルス; 自己抗体

1) Soluble immune complexes were determined by a anti-C3 solid-phase enzyme-linked immunosorbent assay (anti-C3 ELISA) and were found to be significantly higher on average in patients with pustulosis palmaris et plantaris (PPP) and/or stemo-cost-clavicular hyperostosis (SCCH) than in normal controls. There was a close relationship between the degree of improvement in the skin lesions in PPP patients and in pains in SCCH Patients and the changes in soluble immune complexes after tonsillectomy.2) Titers of anti-keratin antibodies (AKA) involving IgG and IgM fractions were determined by ELISA and found to be significantly higher on average in patients with PPP than normal controls. Positive rates of IgG-AKA and IgM-AKA in sera of PPP patients were 50% and 66%, respectively. Following tonsillectomies, patients with high IaM-AKA titers that decreased after surgery were found to benefit by a marked improvement in their skin lesions. There was a close relationship between the degree of improv … More ement in the skin lesions and the changes in IgM-AKA titers after the operation.3) Epstein-Barr virus (EBV) transformed tonsillar lymphocytes were found to produce autoantibodies against nuclear envelope and cytoskeletal filaments, nucleolus and cytoskeletal filaments of human squamous cell carcinoma cell line (Hep2 cell).4) Bacteriologic studies on tonsil of patients with PPP, chronic tonsillitis (CT), and healthy controls revealed that Streptococcus sanguis was isolated in significantly higher rate in PPP patients than in CT patients and healthy controls, and that IgG-antibody titer against bacterial antigen of Streptococcus salvarius was significantly lower in PPP patients than in CT patients and healthy controls.5) Quantitative immunohistologic study on tonsil by the image analysis revealed that IgM-, IgG-and IgD-positive B cells were significantly increased in PPP patients than in CT patients and healthy controls, and that IgM-and IgD-positive B cells and CD4-positive T cells were significantly increased in patients with improvement of skin lesions after tonsillectomy.6) Measurements of soluble immune complexes and IgM-anti-keratin antibody titer in sera of patients in combination with the conventioual tests were thought to be very useful for the preoperative diagnosis of tonsillar focal infections and for the post-operative counseling. Less

1)用抗C3固相酶联免疫吸附试验(anti-C3-EL ISA)检测可溶性免疫复合体，发现掌底脓疱病(PPP)和(或)干性锁骨增生症(SCCH)患者的可溶性免疫复合体平均水平明显高于正常对照组。PPP患者皮损的改善程度和SCCH患者的疼痛程度与扁桃体摘除术后可溶性免疫复合体的变化密切相关。2)用ELISA法检测PPP患者血清中涉及免疫球蛋白和免疫球蛋白M组分的抗角蛋白抗体(AKA)滴度，发现PPP患者的AKA平均滴度明显高于正常对照组。PPP患者血清中Ig G-AKA和Ig M-AKA的阳性率分别为50%和66%。扁桃体切除术后，IAM-AKA滴度较高但术后下降的患者被发现受益于他们的皮肤损害的显著改善。…的改善程度与治疗效果密切相关3)EB病毒(Epstein-Barr Virus，EBV)转化的扁桃体淋巴细胞可产生抗人鳞状细胞癌细胞系(Hep2细胞)核膜和细胞骨架细丝、核仁和细胞骨架细丝的自身抗体。4)PPP、慢性扁桃体炎(CT)和健康对照组扁桃体细菌学研究显示，PPP患者扁桃体血链球菌的分离率明显高于CT患者和健康对照组。5)扁桃体图像定量免疫组织学研究显示，PPP患者扁桃体中IgM、Ig G和Ig D阳性的B细胞明显高于CT患者和正常对照组。扁桃体切除术后皮损改善的患者血清中IgM、IgD阳性B细胞和CD4阳性T细胞明显增加。6)检测患者血清中的可溶性免疫复合物和IgM抗角蛋白抗体效价，并结合常规检测，对扁桃体局灶性感染的术前诊断和术后辅导有重要意义。较少

项目成果

松山秀明,山中昇: "病巣性扁桃炎における免疫組織学的研究-画像解析による定量的研究-" 日本耳鼻咽喉科学会会報. 93. 6-17 (1990)

Hideaki Matsuyama、Noboru Yamanaka：“局灶性扁桃体炎的免疫组织学研究 - 使用图像分析的定量研究”日本耳鼻喉科学会通报 93. 6-17 (1990)。

松山 秀明,山中 昇: "病巣性扁桃炎における免疫組織学的研究ー画像解析による定量的研究ー" 日本耳鼻咽喉科学会会報. 93. 6-17 (1990)

Hideaki Matsuyama、Noboru Yamanaka：“局灶性扁桃体炎的免疫组织学研究 - 使用图像分析的定量研究”日本耳鼻喉科学会通报 93. 6-17 (1990)。

Yamanaka N, Matsuyama H, Harabuchi Y, and Kataura A.: "Distribution of lymphoid cells in tonsillar compartments in relation to infection and age - A quantitative study using image analysis" Act Otolaryngol (Stockh).

Yamanaka N、Matsuyama H、Harabuchi Y 和 Kataura A.：“与感染和年龄相关的扁桃体区室中淋巴细胞的分布 - 使用图像分析的定量研究”Act Otolaryngol (Stockh)。

Yamanaka N,etal: "Tonsillectomyーinduced changes in antiーKeratin antibodies in patients with puspulosis palmaris at plantaris:a clinical correlation" Archives of Otos RhinoーLaryrigology. 246. 109-112 (1989)

Yamanaka N,etal：“扁桃体切除术引起的跖部脓疱病患者抗角蛋白抗体的变化：临床相关性”Otos Rhino-Laryrigology 档案 246. 109-112 (1989)。

Matsuyama H and Yamanaka N.: "Immunohistological study on human palatine tonsils in cases with pustulosis palmaris et plantaris - The quantitative study by image analyzer -" J Otolaryngol Jpn. 93 :. 6-17 (1990)

Matsuyama H 和 Yamanaka N.：“掌跖脓疱病患者腭扁桃体的免疫组织学研究 - 通过图像分析仪进行的定量研究 -”J Otolaryngol Jpn。