Androgen matabolism in normal and malignant laryngeal tissue

本研究以鸡的Sylynx（白色Japanese reghon）、小鼠的淋巴组织和人的淋巴组织为研究对象，探讨这些组织是否是雄激素作用的靶点之一。本文通过应用~ 3 H-睾酮、放射自显影、体内注射研究或组织培养等方法，认为喉正常组织和喉恶性组织，尤其是喉正常组织和喉恶性组织的上皮细胞，是雄激素的靶器官或组织之一。免疫组化结果显示8例喉癌标本中2例抗雄激素抗体阳性细胞。这些结果提示andogrn在正常喉的发育过程中起重要作用，在喉癌的发生和/或生长过程中可能是致癌因子或辅助因子之一，7例喉癌中有1例可移植到小鼠体内，但该株喉癌对雄激素和其他类固醇激素无反应性，我们正在研究检测喉癌中雄激素受体的mRNA，已检测了5例喉癌细胞中雄激素受体的产生，但尚未成功。

Sylynx of Hen (White Japanese reghon), larngeal tissue of mice and human were used and studied to detect whether these tissues are one of targets for androgen. By utilizing ^3H-testosterone, autoradiography and in vivo injection study or tissue culture method, we concluded that noramal and malignant laryngeal tissue, especially epithelium of these tissues, are one of target organs or tissues for androgen. Farthermore, oxidized silver grains demonstrating radio labeled steroid was observed in the nucleus of cells as the same phenomena as obserbed in well known steroid target tissues.Our immunohistochemical studies demonstrated anti-androgen antibody Positive cell in two out of 8 laryngeal carcinoma specimens. These phenoena implies that andogrn plays an important role in normal laryngeal development and as one of carcinogenic factors or cofactors in laryngeal carcinoma development and/or growth.One out of 7 laryngeal carcinomas could be planted mude mice, and however this strain did not show reactivity to androgen and other steroids.We are studying to ditect mRNA of androgen recepter in laryngeal carcinoma, 5 samples have been examined to determine production of androgen recepter in laryngeal carcinoma cell and have not succeeded in this point yet.