Larynx Cell Biological Characteristic Cellular Sex Steroid Signal Transduction and Inhibition of Cell Growth

喉部是许多动物发声的重要器官。语音音高存在性别差异。男性的声音比女性低沉，男性喉癌的发病率也高于女性。这一现象表明喉部的生物学特性是机械诱导的，其特异性是在性类固醇作用控制下的器官。然而，喉部与类固醇之间的相互生物学关系仍不清楚。例如，我们甚至不知道喉癌是否有特定的性类固醇受体蛋白，也不知道喉癌的生长是否受类固醇作用的控制。本研究表明，人喉部上皮细胞，特别是靠近基底膜的上皮细胞，以及喉部鳞癌细胞在细胞核上摄取性类固醇。此外，培养的喉上皮癌细胞（Hep-2）对性类固醇敏感，细胞分裂受到干扰，导致其死亡。在这种癌细胞死亡过程中，存在特异性雄激素受体（AR）基因的转录，与CBP和SRC作为转录辅激活因子相关，没有TIF2，但有AR翻译的运气。这些数据表明，只有AR受体的mRNA能够适应这种细胞证据，而前列腺中已知的AR特异性蛋白在喉部器官中并不起重要作用。微胡同iRNA数据显示雄激素诱导细胞死亡上调miR 27a、miR 21、miR 23a、miR 16、miR 30c、miR 30a 5p、miR 30d、miR 19b、miR 30e 5p、miR 7086、miR 175p的iRNA。然而，所有这些iRNA都不被认为与AR基因有直接或间接的交流。性类固醇对喉部作用的临床认识是非常重要的，需要进一步的研究。

Larynx is an important organ to induce voice of many animals. There is a sexual differentiation on phonetic pitch. Male voice is deeper than women's and there is also higher incidence of male laryngeal cancer more than female. This phenomenon implies that laryngeal biological characteristics are mechanically induced their specificity as the organ under the control of sex steroid action. The mutual biological manner between larynx and steroids, however, has been still obscure. For example we have not known even that laryngeal carcinomas have the specific sex steroid receptor protein or not, and also that the growth are controlled by steroid action or not. This study has indicated that the human laryngeal epithelial cells, especially bordering on basement membrane, and also laryngeal squamous carcinoma cells uptake sex steroids on the nuclei. Furthermore, cultured laryngeal epithelial carcinoma cells (Hep-2) are sensitive to sex steroid and cells are interfered their division to induce their death. On the process of this carcinoma cell death, there is the transcription of the specific androgen receptor (AR) gene, relating with CBP and SRC as transcription co activator, without TIF2, but the luck of AR translation. This data implies that only the mRNA of AR receptor can accommodate this cellular evidence, and the specific protein of AR, well known in the prostate, do not play an important roll on laryngeal organ. Micro alley iRNA data showed that androgen induce cell death up regulates iRNAs of has miR 27a、 has miR 21、 has miR、 23a, has miR 16、 has miR 30c, has miR 30a 5p, has miR 30d, has miR l9b, has miR 30e 5p, ambi miR 7086 has miR 17 5p. All of these iRNA, however, are not considered to have directly and indirectly communication with AR gene. Clinically the knowledge of sex steroid action on the larynx is very important and next step study is needed.