Basic and Clinical Study of Arachidonic Acid Metabolism in Pathogenesis of Nasal Polyps

花生四烯酸代谢在鼻息肉发病机制中的基础与临床研究

基本信息

批准号：
63570812
负责人：
SHIMA Tetsuya
金额：
$ 1.28万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

Arachidonic acid metabolites in nasal polyps were analyzed. Leukotriene (LT) B4, LTC4, LTD4, LTE4, 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, Prostaglandin (PG) E2, 6-keto-PG Fla and thromboxane (TX) B2 were quantitated. Both 12-HETE and 15-HETE were detected dominantly in those metaboites. In addition, the enzyme activities of nasal polyps on ^<14>C-labelled arachidonic acid were measured by radiochromatoscanning. Predominancy of 12-and 15-lipoxygenase activities in the polyps was also noted. It has been considered that LTB4 and 5-HETE promoted the inflammatory cell migration and LTC4, LTD4 and LTE4 facilitated the permiability of the vessels which caused edema occurring in the polyps.In order to investigate the cytotoxic activity of 12- and 15-HETE on the endothelial cells, we established a cell culture of the endothelial cells derived from nasal polyps and pursued the influence of 12- and 15-HETE on growth of the cultured endothelial cells. However, no significant inhi … More bition of the cell growth was observed. Further examination towards these two metabolites was required because of their predominance in the group of arachidonic acid products in nasal polyps.Cell culture of ciliated cells were performed to determine the origin of 12-and 15-HETE. ^<14>C-labelled arachidonic acid was used as a substrate. The existence of 12- and 15-lipoxygenase activities in the cultured ciliated cells were recognized. It was conceivable that the ciliary epithelium in nasal polyps produced 12- and 15-HETE.Nasal polyps accompanied with aspirine-induced asthma were treated by local steroid injection. Remarkable reduction of the size of the polyps was noted. And also, serious decrease of the number of migrated eosinophlic granulocytes was observed in morphological examination. These evidence indicated the characteristic pathogenesis of this type of polyps.Application of Nd-YAG LASER for polypotomy was attained. The LASER irradiation have no significant influence for arachiconic acid metabolism. Compared to the ordinary surgery, the polypotomy using Nd-YAG LASAR achieved more improvement of clinical symptoms during the long term follow. Less

分析了鼻息酸酯代谢产物。白细胞（LT）B4，LTC4，LTD4，LTE4，5-羟基乙酸乙烯烯酸（Hete），12-Hete，15-Hete，15-Hete，Prostaglandin（PG）E2，6-酮PG FLA和TX）B2（TX）B2被定量。在这些代泊人中主要检测到12-Hete和15-Heete。另外，通过放射性抗体测量了鼻息息含量 ^<14> C标记的花生四烯酸的酶活性。还指出了息肉中12-脂氧合酶活性的主要率。 It has been considered that LTB4 and 5-HETE promoted the inflammatory cell migration and LTC4, LTD4 and LTE4 prepared the permission of the Vessels which caused edema occurring in the polyps.In order to investigate the cytotoxic activity of 12- and 15-HETE on the endothelial cells, we established a cell culture of the endothelial cells derived from nasal polyps and pursued the influence of 12- and 15-HETE on growth of培养的内皮细胞。但是，没有观察到细胞生长的更多含量。由于它们在鼻息肉中的蛛网膜酸产物中占主导地位，因此需要对这两个代谢产物进行进一步检查。进行了纤毛细胞的细胞培养以确定12和15-HETE的起源。 ^<14> C标记的花生四烯酸用作底物。认识到培养的纤毛细胞中的12-和15-脂氧合酶活性的存在。可以想象的是，鼻息肉中的睫状上皮产生了12-五和15-螺旋。通过局部类固醇注射治疗了用毒素诱导的哮喘完成的鼻息。注意到息肉的大小显着减少。而且，在形态学检查中观察到了迁移的嗜酸性粒细胞数量的严重减少。这些证据表明，这种类型的息肉的特征发病机理。附着ND-YAG激光器进行了多型息肉。激光辐照对蛛网膜酸代谢没有显着影响。与普通手术相比，使用ND-YAG LASAR的多型切开术在随后的长期中可以改善临床症状。较少的