Studies on ecological activity and enzymes of periodontopathogens

1.赭色二氧化碳噬纤维菌的生态效应、抗菌物质的纯化及性质。C.细胞相关抗菌物质。ochracea No.26和M-12纯化了83倍。该物质的分子量为100，000，其等电点为8.0。该物质对口腔链球菌和痤疮丙酸杆菌的生长有抑制作用。2.牙周致病菌粘多糖酶和神经氨酸酶的纯化及性质。（i）从细胞提取物中纯化了1，920倍的解肝素拟杆菌的肝素酶。该酶的分子量为64，000，其等电点为9.5。最适pH为7.0。只有肝素和硫酸乙酰肝素被酶降解。（ii）口腔拟杆菌的细胞外透明质酸酶被纯化12，000倍。该酶的分子量为75，000，其等电点为7.6。最适pH为5.5。只有透明质酸被酶降解。（iii）牙龈拟杆菌的细胞相关神经氨酸酶被纯化230倍。该酶的分子量为55，000，其等电点为4.5。最适pH为5.8。用纯化的酶处理的红细胞被中间类杆菌细胞凝集。由于在B中发现了神经氨酸酶活性。3.口腔密螺旋体的生物学特性及蛋白酶活性。T.使用合成基质发现了从牙周炎病变中分离的齿垢。通过离子交换层析可以分离出各种酶，这表明该有机体的蛋白水解系统具有很大的多样性。

1.Ecological effects, and purification and properties of antibacterial substance of Capnocytophaga ochracea. Cell-associated antibacterial substance of C. ochracea No.26 and M-12 was purified 83-fold. Molecular weight of this substance was 100,000 and its isoelectric point was 8.0. The substance inhibited bacteriostatically the growth of oral streptococci and Propionibacterium acnes.2.Purification and properties of mucopolysaccharidases and neuraminidase of the periodontopathogens. (i)Heparinase of Bacteroides heparinolyticus was purified 1,920-fold from cell extracts. Molecular weight of the enzyme was 64,000 and its isoelectric point was 9.5. Optimum pH for the activity was 7.0. only heparin and heparan sulfate were degraded by the enzyme. (ii)Extracellular hyaluronidase of Bacteroides oralis was purified 12,000- fold. Molecular weight of the enzyme was 75,000 and its isoelectric point was 7.6. Optimum pH for the activity was 5.5. Only hyaluronic acid was degraded by the enzyme. (iii)Cell-associated neuraminidase of Bacteroides gingivalis was purified 230-fold. Molecular weight of the enzyme was 55,000 and its isoelectric point was 4.5. Optimum PH for the activity was 5.8. Red blood cells treated with the purified enzyme were agglutinated by cells of Bacteroidesintermedius. Since neuraminidase activity was found in B. heparinolyticus and oral Treponema denticola, purification of each enzyme was undertaken.3.Biological properties and protease activity of oral Treponema denticola. Collagenolytic, trypsin-like, and chymotrypsin-like activity of T. denticola isolated from lesions of periodontitis were found using synthetic substrates. Each enzyme could be separated by ion-exchange chromatography, which suggests the proteolytic system of this oraganism, is rather diverse.