Dynamic mechanical-properties and ATPase activities in masticatory muscles.

咀嚼肌的动态机械特性和 ATP 酶活性。

• 批准号: 63570881
• 负责人: SAEKI Yasutake
• 金额: $ 1.41万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570881/
• 关键词: Masticatory muscle; Cross-bridge kinetics; ATPase activity; Shortening velocity; isometric tension; Cross-bridge Kinetics; 力学特性; ATDase活性

To evaluate the dynanic mechanical-properties of contractile system and the ATPase activity in jaw muscles, we analyzed the tension transients in response to small ( 1% of muscle length ) step and sinusoidal length perturbations, and the Ca^<2+> sensitivity of isometric tension development, ATPase activity and maximum shortening velocity (Vmax) in guinea pig glycerinated masseter, temporal and digastric muscles, using a newly developed length servocontrol system. The transient tension responses could be approximated by three exponential functions, each of which has a time constant, T_1, T_2, T_3 in the order of large to small, as has been observed in other skeletal muscles. T_2 and T_3, which are believed to reflect the rate of cross-bridge cycling, were different among the three muscles (in the digastric in the temporalis in the masseter) when the preparations were activated maximally. These results indicate higher cross-bridge cycling rate in the masseter and lower rate in the digastric muscle. The isometric tension increased in a sigmoid fashion with an increase in Ca^<2+> concentration from about pCa 6.6 to pCa 5.8 in all of the three muscles. The ATPase activity also increased sigmoidally with an increase in Ca^<2+> concentration from slightly above pCa 6.6 to slightly below pCa 5.8. The tension cost (ATPase activity / isometric tension) estimated from above results was lower in the digastrib than in the temporalis or masseter, indicating more economical ATP consumption in the digastric. Vmax increased, with an increase in Ca^<2+> concentration (i.ei., with an increase in isometric tension), and greater in the masseter than in the other two muscles when compared at the same developed tension level. These results seem to be consistent with the results obtained from the transient tension response analysis.

为了评估收缩系统的动态力学特性和颌肌中的ATPase活性，我们使用一种新的长度伺服控制系统，分析了小步长(肌肉长度的1%)和正弦长度扰动时的张力瞬变，以及豚鼠甘油咬肌、颞肌和二腹肌的等长张力发展、ATPase活性和最大缩短速度(Vmax)的敏感性。瞬时张力响应可以用三个指数函数来近似，每个指数函数的时间常数T_1、T_2、T_3按大到小的顺序排列，就像在其他骨骼肌中观察到的那样。T_2和T_3被认为是反映跨桥骑行速度的指标，当准备工作最大限度地激活时，三块肌肉(咬肌的二腹肌)之间的T_2和T_3是不同的。这些结果表明，咬肌的跨桥循环率较高，二腹肌的跨桥循环率较低。随着Ca~(2+)浓度从约Pca 6.6增加到Pca 5.8，三种肌肉的等长张力均呈S形增加。随着Ca~(2+)浓度从略高于PCA 6.6到略低于PCA 5.8，ATPase活性也呈S型增加。根据上述结果估算的张力成本(ATPase活性/等长张力)在二腹肌中比在颞肌或咬肌中要低，这表明二腹肌中的ATP消耗更经济。Vmax随着钙离子浓度的增加(即等长张力的增加)而增加，在相同的张力水平下，咬肌的Vmax大于其他两个肌肉的Vmax。这些结果似乎与由瞬时拉力响应分析得到的结果一致。

项目成果

C.H. Paik: "Increase in vertical dimension alters mechanical properties and ATPase activity in guinea pig masseter." Am. J. Orthod.

C.H.

K.Shiozawa: "Effects of mechanical stimulation of the tongue and electrical stimulation of the glossopharyngeal nerve on the activity of the inhrahyoid muscle in the frog,Rana Catesbeiana." Jpn.J.Oral Biol.31. 745-748 (1989)

K.Shiozawa：“机械刺激舌头和电刺激舌咽神经对青蛙舌骨肌活动的影响。”

塩沢光一: "カエル舌刺激で胸骨舌骨筋に誘発される反射応答の比較" 歯基礎誌. 31. 54 (1989)

Koichi Shiozawa：“青蛙舌头刺激引起的胸骨舌骨肌反射反应的比较”，《牙科基础杂志》31. 54 (1989)。

C.H.Paik: "Increase in vertical dimension alters mechanical properties and ATPase activity in guinea pig masseter." Submitted to Am.J.Orthod.

C.H.Paik：“垂直尺寸的增加改变了豚鼠咬肌的机械特性和 ATP 酶活性。”

K. Shiozawa: "Effects of mechanical stimulation of the tongue and electrical stimulation of the glossopharyngeal nerve on the activity of the inhrahyoid muscle in the frog, Rana catesbeiana." Jpn. J. Oral Biol. 31, 1989, 745-748.

K. Shiozawa：“舌头的机械刺激和舌咽神经的电刺激对青蛙舌骨内肌活动的影响。”