Effect of Drugs for Intravenous Sedation on Human Neutrophil Chemiluminescence

化学发光是氧自由基产生的一个指标，氧自由基是一种重要的杀菌机制。本文研究了苯二氮类化合物安定对中性粒细胞化学发光的影响。在中性粒细胞活化调理的酵母聚糖后测量化学发光。在体外研究中，我们发现地西泮在1 μ g/ml-40 μ g/ml浓度范围内可使化学发光减弱，且这种减弱作用具有剂量依赖性。在临床应用中，我们观察了健康志愿者静注安定（0.2mg/kg）后的血药浓度及安定对中性粒细胞化学发光的影响。地西泮给药后3分钟的平均血药浓度为14.1 <plus-minus>± 1.9 μ/ml。我们发现在静脉注射安定镇静时，化学发光被短暂抑制

The chemiluminescence is an index of oxygen free-radical generation, which is an important bactericidal mechanism. The effect of benzodiazepine compound, diazepam on human neutrophil chemiluminescence was evaluated. Chemiluminescence was measured after neutrophil activation of the opsonized zymosan . ht emission was measured using an ATP photometer.In vitro studies, we found that diazepam produce reductions in chemiluminescence at concentrations of 1 ug/ml to 40ug/ml and the depression was dose dependent. This inhibitory effect of diazepam was reversible and chemiluminescence was recovered after washing out of diazepam.In clinical practice, we investigated plasma concentrations and the effect of diazepam on human neutrophil chemiluminescence in healthy volunteers received intravenous sedation with diazepam (0.2mg/kg). Mean plasma umiconcentration at three minutes after administration of diazepam was 14.1 <plus-minus>1.9 mu/ml. we found the chemiluminescence was inhibited transiently undergoing intravenous sedation with diazepa