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Comparison of various analytical methods for determination of total and ionic fluoride in biological samples.

测定生物样品中总氟化物和离子氟化物的各种分析方法的比较。

基本信息

批准号：
63570968
负责人：
TOMITA Misako
金额：
$ 1.22万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

The objective of this study was to find a suitable method for the determination of total and ionic fluoride(F) in biological samples.Six procedures were employed for the fluoride analysis; the fluoride ion selective electrode (IE method), the microdiffusion-gas chromatography (Dif -GC method), AlF molecular absorption spectrometry (AlF method), the low temperature ashing-GC (LTA-GC method) had previously been reported, while the pyrohydrolysis-GC (PyH-GC method) and the direct extraction-GC (DE-GC method) were newly applied to this study.We investigated the operating conditions of the apparatus for PyH and the recovery of F using calf serum. In addition, the DE-GC method was applied to determine ionic F in the hard tissues of rats which had been given Diflunisal (organic F compound).The above mentioned methods were tested by combining some of them for determining total and ionic F in various samples; the samples used were those containing organic F compounds and the tissues of rats whi … More ch had been given Diflunital. The F values obtained were then compared.The results were as follows: 1. The recovery rates of six organic F compounds were measured by four analytical methods. In comparing the four determining methods, the Dif-GC method using a strong acid proved to be deficient and the AlF method showed low recovery rates for half of the organic F compounds examined. Although the PyH-GC method showed a low recovery rate for 4-fluorobenzophenone which has a low melting point, it showed fairly good recovery rates for the other organic F compounds; the LTA-GC method showed the best recovery rate. 2. The total F values in the plasma and soft tissues of rats resulted in an excellent agreement between the samples pretreated with LTA and those with PyH. This result indicates that both methods are suitable for determining F in Diflunisal and its metabolite in the plasma and soft tissues. However, it is better to use the PyH-GC method for determination of total F in biological samples because it is more rapid and simpler than the LTA-GC method. 3. The ionic F in the hard tissues of rats showed good agreement between the samples determined by the Dif-GC and the DE-GC method. From this result, the DE-GC method is preferable to the Dif-GC method because it is very simple and rapid. Less

本研究的目的是寻找一种适合测定生物样品中总氟和离子氟(F)的方法。氟化物分析采用了六种方法；氟离子选择电极法（IE）、微扩散气相色谱法（Dif -GC法）、AlF分子吸收光谱法（AlF法）、低温灰化气相色谱法（LTA-GC法）等方法已有报道，而热水解气相色谱法（PyH-GC法）和直接萃取气相色谱法（DE-GC法）是新应用的。我们研究了PyH检测装置的操作条件和用牛血清回收F的方法。此外，采用DE-GC法测定给药双氟尼柳（有机氟化合物）大鼠硬组织中的离子氟。将上述几种方法结合起来，对不同样品中总F和离子F的测定进行了试验；所使用的样品是含有有机氟化合物的样品和大鼠的组织。然后比较得到的F值。实验结果如下：1.实验结果表明：采用4种分析方法测定了6种有机含氟化合物的回收率。在四种测定方法的比较中，使用强酸的Dif-GC法被证明是有缺陷的，而AlF法对所检测的有机F化合物的回收率低。PyH-GC法对熔点较低的4-氟苯甲酮的回收率较低，但对其他有机F类化合物的回收率较好；LTA-GC法回收率最高。2. 大鼠血浆和软组织中的总F值与经LTA预处理的样品和经PyH预处理的样品非常吻合。结果表明，两种方法均适用于测定血浆和软组织中双氟尼柳及其代谢物中的氟。但是，PyH-GC法比LTA-GC法更快速、更简单，更适合用于生物样品中总F的测定。3. Dif-GC法和DE-GC法测定的大鼠硬组织中离子F的含量基本一致。从这个结果来看，DE-GC法比Dif-GC法更适合，因为它非常简单和快速。少