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Progress of the Metabolic Pathway According to the Evolution of Organisms-Biosynthesis of Vitamin B Group and Coenzymes-

生物体进化代谢途径的进展-维生素B族和辅酶的生物合成-

基本信息

批准号：
63571070
负责人：
KUMAOKA Hiroshi
金额：
$ 1.28万
依托单位：
Mukogawa Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

项目摘要

1. Tne Biosynthetic Pathway of ThianinTwo ^<15>N-atoms of DL-[u, 3- ^<15>N]histidine were incorporated into the N-3 and amino-N atom at C-4 of the pyrimidine of thiamin. L-[2- ^<14>C]Histidine was incorporated directly into the pyrimidine. In contrast, the ^<15>N-atom of DL-[amino- ^<15N>]histidine and ^<14>C-atom of [U- ^<14>C]histidine, except C-2 of histidine, were not incorporated into the pyrimidine. We conclude that N-1, C-2, and N-3 of histidine are the origins of the N-3, C-4, and amino-N at C-4 of the pyrimidine in thiamin synthesized by Saccharomyces cerevisiae.The competitive investigation with ^<15>NH@_4Cl and various ^<14>N-compounds was carried out. The results revealed the origin of the N-1 atom of pyrimidine was derived from the N-1 atom of pyridoxine. The similarity of the stracture of the pyrimidine and pyridoxine suggests the N-1, C-2, and methyl carbon at C-2 of the pyrimidine was dirived from the N-1, C-2, and methyl carbon at C-2 of pyridoxine.In procaryotes, N, C … More -1, and C-2 of glycine are incorporated into N-1, C-4, and C-6 of the pyrimidine, respectively. We studied the incorporation of glycine into the pyrimidine in a eucaryote, Saccharomyces cerevisiae. The results rerealed that[1- ^<14>C]glycine was not incorporated into the pyrimidine, whereas[2- ^<14>C]glycine was incorporated into the pyrimidine at C-4 via histidine, a precursor of the C-4 of pyrimidine.2. The Biosvnthetic Pathway of the PyridoxineWe studied incorporation of L-[U- ^<14>C]alanine, [2- ^<14>C]pyruvate, [2- ^<14>C]acetate, [ ^<14>C]formate and[2- ^<14>C]glycerol into pyridoxine in Flavobacterium sp. No. 238-7. The respective radioactive carbon of alanine, pyruvate, acetate, formate and glycerol was incorporated into pyridoxine with high dilution. These findings suggest that the compounds tested are not the direct precursor.It has been reported that the C-5 and the hydroxymethyl carbon at C-5 of the pyridoxine is originated from the carbon atoms of glycolaldehyde. We revealed the active glycolaidehyde was dirived from the C-1 and C-2 atoms of fructose and incorporated into the C-5 and hydroxymethyl carbon at C-5 of the pyridoxine in Escherichia coli. Less

1.硫胺素的生物合成途径DL-[u，3-^ 15 N]组氨酸的两个^ 15 N-原子被掺入到硫胺素嘧啶的C-4处的N-3和氨基-N原子中。将L-[2-14C]组氨酸直接掺入嘧啶中。相反，DL-[氨基- 15 N ]组氨酸的 15 N-原子和[U- 14 C]组氨酸的 14 C-原子，组氨酸的C-2除外，没有并入嘧啶中。我们得出结论，组氨酸的N-1、C-2和N-3是酿酒酵母合成的硫胺素中嘧啶的N-3、C-4和C-4氨基-N的起源。进行了与^<15>NH@_4Cl和各种^<14>N-化合物的竞争性研究。结果表明，嘧啶的N-1原子来源于吡哆醇的N-1原子。嘧啶和吡哆醇结构的相似性表明嘧啶的N-1、C-2和C-2处的甲基碳源自吡哆醇的N-1、C-2和C-2处的甲基碳。在原核生物中，甘氨酸的N、C…更多-1和C-2被掺入嘧啶的N-1、C-4和C-6中，分别。我们研究了真核生物酿酒酵母中甘氨酸掺入嘧啶的情况。结果再次表明，[1-14C]甘氨酸没有掺入嘧啶中，而[2-14C]甘氨酸通过组氨酸(嘧啶C-4位的前体)掺入嘧啶的C-4位。2．吡哆醇的生物合成途径我们研究了L-[U-^<14>C]丙氨酸、[2-^<14>C]丙酮酸、[2-^<14>C]乙酸、[^<14>C]甲酸和[2-^<14>C]甘油在黄杆菌中掺入吡哆醇。第 238-7 号。将丙氨酸、丙酮酸、乙酸盐、甲酸和甘油各自的放射性碳掺入高稀释度的吡哆醇中。这些发现表明所测试的化合物不是直接前体。据报道，吡哆醇的C-5和C-5处的羟甲基碳源自乙醇醛的碳原子。我们发现，活性乙醇醛源自果糖的 C-1 和 C-2 原子，并掺入大肠杆菌中吡哆醇的 C-5 和 C-5 羟甲基碳中。较少的