Development of Micro Analytical System of Hydroperoxides in Lipids

脂质中氢过氧化物微量分析系统的研制

• 批准号: 63860011
• 负责人: MEGURO Hiroshi
• 金额: $ 4.48万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B).
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63860011/
• 关键词: hydroperoxide; dertermination; fluorometry; HPLC; diphenyl pyrenyl phosphine; diphenylー1-pyrenylphosphine; high performance liquid chromatography; triacyl glycerol; ジフェニルー1ーピレニルホスフィン; ヒドロペルオキシド; 蛍光分析システム

Extremely sensitive fluorometry of lipid hydroperoxides (HPO) in biological samples were developed using diphenyl-1-pyrenylphosphine (DPPP), a novel reagent synthesized us.1. A batch method is developed to determine total HPO in edible fats and oils. The mixture of the reagent and sample solutions was reacted at 60^ﾟC for 60 min in the dark. After the reaction, DPPP oxide was determined by fluorometry. By the method, subnano mole level of lipid HPO could be determined with mg order of a sample.2. HPLC systems are developed by using post column detection unit which composed of a pump to send the reagent solution, reaction coil and a fluorometer. By the systems, HPO were determined at pico mole level.(1) Triacylglycerol (TG) HPO : TG-monoHPO were separated to several peaks on their partition numbers on an ODS colomn. On a phenylated silicagel column, they gave a single peak to made possible to determined total TG-monoHPO.(2) Phosphatidylcholine (PC)- and Phosphatidylethanolamine (PE) -HPO : PC- and PE-HPO were separated by two columns, a silicagel and an aminopropyl columns, connected directly. This system was applicable to determine PC-HPO in plasma samples.

使用二苯基-1-苯基膦（DPPP）开发了生物样品中脂质氢过氧化物（HPO）的极敏感的荧光测定法，这是一种新型的试剂合成的US.1。开发了一种批处理方法来确定可食用脂肪和油中的总HPO。在黑暗中以60^C的60分钟反应试剂和样品溶液的混合物。反应后，通过荧光测定法确定DPPP氧化物。通过该方法，可以用样品的Mg级确定脂质HPO的亚南诺分子水平。2。 HPLC系统是通过使用由泵组成的后柱检测单元来开发的，该单元通过泵组成来发送试剂溶液，反应线圈和荧光计。通过系统，在PICO摩尔水平上确定HPO。（1）将三酰基甘油（TG）HPO：TG-MONOHPO分离为ODS COLOMN上的分区数上的几个峰。在苯基化的硅氧基柱上，它们给出了一个峰，以确定总TG-MONOHPO。（2）磷脂酰胆碱（PC） - 和磷脂酰甲醇胺（PE）-HPO：PC-和PE-HPO通过两柱，一个硅酮和氨基蛋白蛋白蛋白蛋白蛋白蛋白，可连接。该系统适用于在血浆样品中确定PC-HPO。

项目成果

Hiroshi MEGURO: "Development of new fluorometrical reagents applicable to food and biological materials" Proceedings of the Third Korea-Japan Joint Symposium on Analytical Chemistry. 153-158 (1989)

Hiroshi MEGURO：“开发适用于食品和生物材料的新型荧光试剂”第三届韩日分析化学联合研讨会论文集。

Kazuaki AKASAKA: "Fluorometric Determination of Triacylglycerol Hydroperoxides at Picomole Levels by HPLC PostーColumn Method with Diphenylー1ーpyrenylphosphine" J.Chromatogr.

Kazuaki AKASAKA：“采用二苯基-1-芘基膦的 HPLC 柱后方法荧光测定皮摩尔水平的三酰甘油氢过氧化物”J.Chromatogr。

Kazuaki AKASAKA: "An aromatic phosphine reagent for the hplc-fluorescence determination of hydroperoxides -determination of phosphatidylcholine hydroperoxides in human plasma -" Analytical Letters. 21 (6). 965-975 (1988)

Kazuaki AKASAKA：“用于氢过氧化物的 hplc 荧光测定的芳香膦试剂 - 人血浆中磷脂酰胆碱氢过氧化物的测定 -” 分析快报。

Kazuaki AKASAKA: "AN AROMATIC PHOSPHINE REAGENT FOR THE HPLCーFLUORESCENCE DETERMINATION OF HYDROPEROXIDES ー DETERMINATION OF PHOSPHATIDYLCHOLINE HYDROPEROXIDES IN HUMAN PLASMA ー" ANALYTICAL LETTERS. 21. 965-975 (1988)

Kazuaki AKASAKA：“用于氢过氧化物的 HPLC 荧光测定的芳香族膦试剂 - 人血浆中磷脂酰胆碱氢过氧化物的测定 -”分析快报 21. 965-975 (1988)。

Kazuaki AKASAKA: "A Simple Fluoromety of Hydroperoxides in Oils and Foods" Agric. Biol. Chem.

Kazuaki AKASAKA：“油和食品中氢过氧化物的简单荧光测定”农业。