Development of new ultra micro analytical system based on the acridine fluorometry

基于吖啶荧光法的新型超微量分析系统的开发

• 批准号: 60860012
• 负责人: MEGURO Hiroshi
• 金额: $ 4.8万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60860012/
• 关键词: N-(9-Acridinyl)maleinide; thiol; 9-Bromomethylacridine; fruorometry; Carboxylic acid; fat; 脂肪; 高速液体クロマトグラフィー; セレノール; N-(9-アクリニジル)マレイミド; セレノシステイン; ゼレノシスチン; メチオニン-γ-リアーゼ; リポ酸; 螢光分析

Two fluorometry based on 1:N(9-Acridinyl)maleimide (NAM) for SH,SeH and SO_2 and 2:9-Bromomethylacridine (9-BrMA) for RCOOH were developed.(1) A new synthetic method for NAM was developed. The yield was greatly improved.(2) A new fluorometric reaction of NAM and SeH was developed. Electrical reaction of -Se-Se- to SeH was combined new dluorometry of seleno cysteins was designed.(3) NAM fluotometry was used in enzymatic analysis of methionine after convertion to homocysteind.(4) A new fruorometric reagent 9-BrMa was used to label carboxylic acid on high performance liquid chromatography. The hydrolysis was also designed to determine the carboxylic acid composition of fat.

开发了基于1：N（9- acridinyl）的甲酰亚胺（NAM）的两个荧光法，用于SH，SEH和SO_2和SO_2和2：9-溴甲基acro（9- brma）的RCOOH。（1）开发了一种新的NAM合成方法。 （2）开发了NAM和SEH的新荧光反应。设计了-se-se-seh的电反应，将新的硒囊蛋白的新dluor测定法组合起来。（3）转化为同叶表后，使用NAM荧光测定法进行了蛋氨酸的酶促分析。（4）一种新的Fruormemptric Geagent New Fruormement regortric 9- brma用于在高性能液体光学上标记高性能的弹盒盒子。水解还旨在确定脂肪的羧酸组成。

项目成果

Kazuaki AKASAKA: Anal. Lett.20(10). 1581-1594 (1987)

赤坂和明：肛门。

Kazuaki AKASAKA: "9-Bromomethylacridine a Novel Fluorescent Labeling Reagent of Carboxylic Group for HPLC" Anal. Lett.20 (10). 1581-1594 (1987)

Kazuaki AKASAKA：“9-溴甲基吖啶是一种用于 HPLC 的新型羧基荧光标记试剂”分析。

Hiroshi OHRUI: "An Improved Syntehsis of N-(9-Acridinyl)maleinide (NAM)" Agric. Bio. Chem.49(3). 885 (1985)

Hiroshi OHRUI：“N-(9-吖啶基)马来酰亚胺 (NAM) 的改进合成”Agric。

Hiroyuki TANNO: Agric. Biol. Chem.49(5). 1517-1518 (1985)

丹野宏之：农业。

Hitoyuki TANNO: "Assay of LMethionine <gamma>-Lyase with N-(9-Acridinyl)maleimide" Agric. Biol. Chem.49(5). 1517-1518 (1985)

Hitoyuki TANNO：“用 N-(9-吖啶基)马来酰亚胺测定 LM 蛋氨酸 <gamma>-裂解酶”Agric。