Analysis of Physiological Properties of Plant Dwarfism

植物矮化的生理特性分析

• 批准号: 01540568
• 负责人: KATSUMI Masayuki
• 金额: $ 1.28万
• 依托单位: International Christian University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540568/
• 关键词: Gibberellin; Cell wall; cell elongation; microtubule; Zea mays, L.; Hemicellulose; Leaf sheath elongation; Dwarfism; IAA; 微小管の配向; セルロ-ス・ミクロフイブリル

1. The relationship between the dwarf habit of growth and cortical microtubule (MT) orientation in mesocotyl cells as affected was examined using a gibberellin (GA) responding dwarf mutant of maize (d5). Mts were observed by immunofluorescence microscopy. MT arrangement in the tangential surface of epidermal cells (EP-t) was oblique and /or longitudinal. The frequency of occurrence of transversely oriented MTs is much less in d5 than normal. In the radial surface of epidermal cells (EP-r), almost all the cells had transversely oriented MTs. In d5 seedlings only a few percent of cells had obliquely oriented MTs. In the radial surface of cortex cells (c-r), all the cells of normal mesocotyls had transversely oriented MTs, while in d5, MTs in considerably a large number of cells showed oblique/longitudinal orientation By GA treatment for 24 h, the growth of d5 mesocotyls was stimulated, and the orientation of MTs in EP-t, EP-r and C-r became transverse.2. The endogenous IAA level in d5 seedlings was lower than that in normal seedlings at the early period of growth, but at later stages the level declined and no significant difference was detected between d5 and normal. GA had no effect on the level in d5 seedlings.3. The content of the hemicellulose B (HB) fraction of d5 seedlings was higher than that of normal seedings during the growth period, and GA treatment resulted in a decrease of HB. The HB of d5 showed a higher molecular-weight distribution than that of normal. GA treatment caused a shift in the distribution to the lower molecular weight range.

1.利用赤霉素（GA）诱导的玉米矮秆突变体（d5），研究了矮秆生长习性与受影响的中胚轴细胞皮层微管（MT）取向之间的关系。免疫荧光显微镜观察Mts。MT在表皮细胞切向面（EP-t）呈斜向和/或纵向排列。横向MT的发生频率在d5比正常情况下低得多。在表皮细胞的放射面（EP-r），几乎所有的细胞都有横向的MT。在d5幼苗只有几个百分比的细胞有倾斜取向的MT。在皮层细胞径向表面（c-r），所有正常中胚轴细胞的MT均为横向取向，而在d5，大量细胞的MT呈斜/纵向取向GA处理24 h后，d5中胚轴的生长受到刺激，EP-t、EP-r和C-r中的MT均为横向取向.生长前期，d5幼苗内源IAA含量低于正常幼苗，但生长后期，内源IAA含量下降，与正常幼苗差异不显著。赤霉素对d5幼苗的含量没有影响. d 5幼苗的半纤维素B（HB）组分含量在整个生长期内均高于正常幼苗，GA处理使HB组分含量降低。第5天的HB显示出比正常高的分子量分布。GA处理引起分布向较低分子量范围的转变。

项目成果

K.ISHIDA and M.KATSUMI: "Effects of gibberellin and ABA on microtubule arrangement in the hypocotyl cells of lightーgrown cucumber seedlings."

K.ISHIDA 和 M.KATSUMI：“赤霉素和 ABA 对光照黄瓜幼苗下胚轴细胞微管排列的影响。”

K.ISHIDA and M.KATSUMI: "Effects of gibberellin and ABA on microtubule arrangement in the hypocotyl cells of lightーgrown cucumber seedlings"

K.ISHIDA 和 M.KATSUMI：“赤霉素和 ABA 对光生黄瓜幼苗下胚轴细胞微管排列的影响”

Katsumi, M.: "The gibberellin control of cell elongation" Gibberellins Edited by N. Takahashi, B. O. Phinney and J. MacMillan. Springer-Verlag, Tokyo. 211-219 (1990)

Katsumi, M.：“赤霉素对细胞伸长的控制”赤霉素由 N. Takahashi、B. O. Phinney 和 J. MacMillan 编辑。

T.SATO and M.KATSUMI: "Expression of dwarfism in the cell wall architecture of the leaf sheath of d5 mutant of Zea mays L."

T.SATO 和 M.KATSUMI：“玉米 d5 突变体叶鞘细胞壁结构中矮化的表达。”

K.ISHIDA and M.KATSUMI: "Arrangement of cortical microtubules in the mesocotyl cells of d_5 and normal plants of Zea mays L.as affected by gibberellin" Plant Physiology.

K.ISHIDA 和 M.KATSUMI：“受赤霉素影响的 d_5 和正常玉米植物的中胚轴细胞中皮质微管的排列”植物生理学。