Functional Analysis of the Jumyo Gene of Paramecium Tetraurelia

四尿草履虫Jumyo基因的功能分析

• 批准号: 01540596
• 负责人: TAKAGI Yoshiomi
• 金额: $ 1.28万
• 依托单位: Nara Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540596/
• 关键词: Paiamecium; P. caudatum; P. multimicronucleatum; Paramecium Growth Factor; ParGF; jumyo mutant; Fetal Bouine Serum; FBS; Paramccium; Paramecium caudatum; Paramecium tetraurelia; ゾウリムシ; 寿命遺伝子; jumyo mutant; 成長因子

1. Excretion of Paramecium Growth Factor from Paramecium caudatum and Paramecium multimicronucleatum.We found last year that the jumyo mutant of P. tetraurelia excreted a Paramecium Growth Factor (ParGF) and successfully purified it. We also evidenced that stocks of P. tetraurelia other than the jumyo mutant excreted a substance functionally equivalent to ParGF. We studied this year if different species of Paramecium might excrete ParGF. Cell-free fluid of the early stationary culture of each stock was concentrated about 100-fold through ultrafiltration. This sample was tested for the ParGF activity by monitoring the restoration of the reduced fission rate of the jumyo mutant in daily reisolation cultures. All of the stocks of P. caudatum and P. multimicronucleatum, including different mating types, were found positive for the excretion of ParGF.2. Cell Division-Promoting Activity of Fetal Bovine Serum.Various mammalian growth factors were tested for the cell division-promoting activity with the assay method described above. So far they were all negative, although each sample was studied only once at a definite concentration. However, their cell division-promoting activity remains still open, because 2% FBS was conspicuously effective for the promotion of cell division of the jumyo mutant.

1。从尾tarparium caudatum和甲状腺甲核核酸杆菌中排出的甲虫生长因子。我们去年发现，假霉菌的jumyo突变体排泄了甲苯丙胺生长因子（PARGF）并成功纯化了它。我们还证明，除jumyo突变体以外的其他毒疟原虫的库存在功能上等效于PARGF。今年，我们研究了如果不同种类的黑甲可能会排泄pargf。每种库存的早期固定培养物的无细胞液通过超滤浓缩约100倍。通过监测每日再分化培养物中的jumyo突变体降低的裂变率，测试了该样品的PARGF活性。发现尾a和多米核假单胞菌的所有库存，包括不同的交配类型，对PARGF.2的排泄呈阳性。胎牛血清的细胞分裂促进活性。通过上述测定方法测试了多种哺乳动物生长因子的细胞分裂促进活性。到目前为止，它们都是负的，尽管仅研究了每个样品以确定的浓度研究一次。但是，它们的细胞分裂促进活性仍然保持开放，因为2％FBS明显有效地促进了jumyo突变体的细胞分裂。

项目成果

TAKAGI,YOSHIOMI: "GROWTH PROMOTING SUBSTANCE SECRETED BY THE JUMYO MUTANT OF PARAMECIUM TETRAURELIA." ZOOLOGICAL SCIENCE. 6. 1210 (1989)

TAKAGI, Yoshiomi：“草履虫 JUMYO 突变体分泌的生长促进物质。”

Kaji, K., Nishi, N., Tanabe, H., Kano, K., Yokoyama, Y. and Takagi, Y.: "Paramecium growth factor (ParGF) is homologous to epidermal growth factor (EGF) and Euplotes mating pheromone Er-1"

Kaji, K.、Nishi, N.、Tanabe, H.、Kano, K.、Yokoyama, Y. 和 Takagi, Y.：“草履虫生长因子 (ParGF) 与表皮生长因子 (EGF) 和游标虫交配信息素同源

TAKAGI,YOSHIOMI: "Excretion of paramecium growth factor (ParGF) by three species of Paramecium."

TAKAGI，Yoshiomi：“三种草履虫分泌草履虫生长因子（ParGF）。”

高木 由臣: "ゾウリムシ三種による細胞成長因子の分泌と哺乳類細胞成長因子への反応" 基礎老化研究. 14. 215-216 (1990)

Yoshiomi Takagi：“三种草履虫的细胞生长因子的分泌和对哺乳动物细胞生长因子的反应”基础衰老研究14。215-216（1990）。

TAKAGI,YOSHIOMI: "The jumyo mutant of Paramecium tetrauvelia secretes growth promoting substancecs)" VIII INTERNATIONAL CONGRESS OF PROTOZOOLOGY PROGRAM AND ABSTRACTS. 119 (1989)

TAKAGI，Yoshiomi：“草履虫的 jumyo 突变体分泌生长促进物质（cs）”第八届国际原生动物学大会计划和摘要。