Development of Novel Synthetic Bilayer System Possessing Chemical Information Transduction and Amplfication Ability

具有化学信息传导和放大能力的新型合成双层系统的开发

• 批准号: 01550579
• 负责人: NAKASHIMA Naotoshi
• 金额: $ 1.47万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01550579/
• 关键词: Bilayer Membrane; Bilayer Electrode; Monolayer Electrode; Functional Electrode; Impedance; Cyclic Voltammogram; Phase Transition; Bilayer Sensor; 情報変換; 分子認識; 蛍光プロ-ブ; クラウンエ-テル; 蛍光性クラウンエ-テル

Biological membranes are composed of lipid bilayer and membrane proteins and act as transducers which convert chemical signals to electrical signals. The aim/purpose of this study is to develop novel molecular recognition and information transduction systems mimic to biological membranes. The summaries are as follows.(1) A mercaptan-containing phosphate lipid was designed and prepared. Monolayer of the lipid was fabricated on gold electrodes via chemisorption from ethanol and their properties were examined by cyclic voltametry. The permeation or diffusion of ferricyanide and ferrocene ammonium into the monolayer on the electrode could be controlled by changing pH of the solution. The electrodes were found to response to alkali earth metal ions.(2) Fundamental examinations on design and fabrication of bilayer devices which transduce molecular information to electric or electrochemical signals were conducted. Quaternary ammonium lipids on patterned electrodes showed drastic change in impedance and capacitance upon heating above phase transition of bilayers. However, primary ammonium lipids exhibited no such phase transition dependence. Despite the very small transition enthalpy, single chain quaternary ammonium film showed the same Tc dependence in impedance measurements as those with double chain lipids. Impedance characteristics of biological lipid films were also investigated. Molecular mechanisms for the obtained results were analyzed by cole-cole plot analyses of impedance.

生物膜由脂双层和膜蛋白组成，充当将化学信号转化为电信号的传感器。本研究的目的是开发新型的模拟生物膜的分子识别和信息传递系统。摘要如下。(1)设计并制备了一种含巯基的磷酸酯脂质。通过乙醇化学吸附法在金电极上制备了脂质单分子膜，并通过循环伏安法对其性质进行了研究。通过改变溶液的pH值，可以控制铁氰化物和二茂铁铵向电极上单分子膜的渗透或扩散。电极被发现响应碱土金属离子。(2)对将分子信息转换为电信号或电化学信号的双层膜器件的设计和制备进行了基础研究。季铵脂质图案化的电极上显示出急剧的变化，在阻抗和电容加热以上的相变的双层。然而，伯铵脂质表现出没有这样的相变依赖性。尽管很小的转变焓，单链季铵膜表现出相同的Tc依赖性的阻抗测量与双链脂质。生物脂质膜的阻抗特性进行了研究。通过阻抗谱的cole-cole图分析，对所得结果的分子机理进行了分析。

项目成果

K.Nakano,I,Moriguchi,N.Nakashima,M.Takagi: "Possible Use of Synthetic chiral Chromophoric Bilayer Membranes As Chemical Transducer" J.Mat.Sci.

K.Nakano，I，Moriguchi，N.Nakashima，M.Takagi：“合成手性发色双层膜作为化学传感器的可能用途”J.Mat.Sci。

K.Nakano,I.Moriguchi,N.Nakashima,M.Takagi: "Possible Use of Synthetic chiral Chromophoric Bilayer Membranes As Chemical Transducer" J.Mat.Sci.

K.Nakano、I.Moriguchi、N.Nakashima、M.Takagi：“合成手性发色双层膜作为化学传感器的可能用途”J.Mat.Sci。

N. Nakashima, Y. Takada, M. Kunitake, O. Manabe: "Assembled Supported Monolayers of Synthetic Lipids on Gold Electrodes via "Ion-Exchange Methods"" J. Chem. Soc, Chem. Commun.845-847 (1990)

N. Nakashima、Y. Takada、M. Kunitake、O. Manabe：“通过“离子交换方法”在金电极上组装支撑的合成脂质单分子层”J. Chem。

Naotoshi Nakashima,Hideo Eda,Masashi Kunitake,Osamu Manabe,Koji Nakano: "Design of a Molecular Bilayer Electrical Device" J.Chem.Soc.,Chem.Commun.(1990)

Naotoshi Nakashima、Hideo Eda、Masashi Kunitake、Osamu Manabe、Koji Nakano：“分子双层电气器件的设计”J.Chem.Soc.、Chem.Commun.（1990）

Naotoshi Nakashima,Hideo Eda,Masashi Kunitake,Osamu Manabe,Koji Nakano: "Design of a Molecular Bilayer Electrial Device" J.Chem.Soc.,Chem.Commun.443-444 (1990)

Naotoshi Nakashima、Hideo Eda、Masashi Kunitake、Osamu Manabe、Koji Nakano：“分子双层电子器件的设计”J.Chem.Soc.、Chem.Commun.443-444 (1990)