权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A study of intraspecific variation and phylogenetic relationships by the analysis of karyotype and isozyme in Allium schoenoprasum

通过核型和同工酶分析葱属种内变异和系统发育关系

基本信息

批准号：
01560023
负责人：
INADA Ikuko
金额：
$ 1.22万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560023/
关键词：
Allium schoenoprasum chives Asatsuki C-banding banding pattern karyotype phylogenetic relationship pollen fertility Allium schoeno prasum L.核型分析アイソザイム分析 Allium schoenoprasum L.C-バンド法

项目摘要

This experiment was carried out in order to elucidate the intraspecific variation in Allium schoenoprasum which contains both Asatsuki and chives. The results are summarized in the following, mainly about cytogenetic experiment.(1) 47 strains of Asatsuki and 8 cultivars of chives were collected.(2) Cytogenetic observation (1) Chromosome number : Almost strains of Asatsuki were either diploid (2n=16) or triploid (2n=24), besides two plants of a strain being 2n=16+lB. All plants of chives were diploid (2n=16). (2) Pollen fertility and PMC meiosis : Some diploid strains of Asatsuki had low pollen fertility but these plants showed the regular meiosis till the tetrad stage. (3) C-banded karyotype : 14 diploid and 6 triploid strains of Asatsuki, 10 plants of 4 cultivars of chives were examined. (i) In diploids of Asatsuki, the eight shorter V-shaped chromosomes were identified after C-banding. These diploid plants were divided into two groups according to the banding pattern of the eight sho … More rter V-shaped chromosomes. As a result of morphological observation, pollen fertility, plant height, tillering and the flower organs were different between the two diploid groups. In triploids, the shorter 12 V-shaped chromosomes were identified with C-banding. (ii) In chives, the eight shorter V-shaped chromosomes were distinguishable, but their banding patterns were different among individual plants. (iii) In C-banded karyotypes of F_1 hybrid plants (chives X Asatsuki, Asatsuki X chives ; 2n=16), as for the length of 8 chromosomes which were identified, on the whole, the chromosomes of chives were shorter than Asatsuki. Although the meiotic behaviors of F_1 hybrid plants suggested that both of Asatsuki and chives had a set of 8 homologous chromosomes, this was not always clear in C-banded karyotypes.(3) Peroxidase isozyme pattern in mature leaves were examined in some strains or plants of Asatsuki and chives. Each of Asatsuki and chives had one characteristic band, the density of which bands and the presence of another band were different among some strains or plants. Less

本试验旨在阐明同时含有赤土和细香葱的香葱的种内变异。现将结果总结如下，主要是细胞遗传学实验。(1)收集了47个Asatsuki株系和8个韭菜品种。(2)细胞遗传学观察（1）染色体数目：除一个株系的两株为2n=16+ 1B外，其余株系均为二倍体（2n=16）或三倍体（2n=24）。韭菜植株均为二倍体（2n=16）。(2)花粉育性和花粉母细胞减数分裂：部分二倍体材料花粉育性较低，但减数分裂规律一直持续到四分体时期。(3)C-带核型：对14个二倍体和6个三倍体的Asatsuki株系，4个韭菜品种的10个植株进行了核型分析。(i)在二倍体Asatsuki中，经C-分带鉴定出8条较短的V形染色体。这些二倍体植株根据其8个染色体的带型可分为两组， ...更多信息后V形染色体。形态学观察结果表明，两个二倍体群体的花粉育性、株高、分蘖和花器官均存在差异。在三倍体中，较短的12条V形染色体用C-带鉴定。(ii)韭菜的8条短V形染色体可区分，但其带型在单株间存在差异。(iii)在韭菜×朝月、朝月×韭菜（2n=16）杂种F_1的C-带核型中，所鉴定的8条染色体的长度，韭菜的染色体总体上短于朝月。尽管杂种F_1的减数分裂行为表明，Asatsuki和韭菜都具有一套8条同源染色体，但在C-带核型中这一点并不总是清楚的。(3)本文研究了几个品种（系）和韭菜成熟叶片的过氧化物酶同工酶谱。韭菜和Asatsuki各有一条特征带，不同品系或不同植株的特征带密度和是否存在另一条特征带存在差异。少