Studies on Trehalase Inhibitor

海藻糖酶抑制剂的研究

• 批准号: 01560130
• 负责人: MURAO Sawao
• 金额: $ 1.22万
• 依托单位: Kumamoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560130/
• 关键词: Inhibitor; Trehalase; 阻害剤

The project was concerned on the novel trehalase inhibitor, Trehalostatin, produced by Amycolatopsis trehalostatica HG-25, which was isolated by the investigators.The molecular formula was determined to be C_<13>H_<22>O_<10>N_2 by SIMS spectrum (m/z 367, [M+H]^+) and elemental analysis. From various spectroscopic data (13C-, 1H-, H, H-COSY, C, H-COSY, NOESY spectra), the structure was elucidated to be an pseudo-disacchaide, composed of alpha-D-glucopyranoside and 5-membered cyclitol moieties. The linkage of the two moieties was proved to be isoureido bond. This structure was a novel one, not yet reported.Trehalostatin specifically inhibits various trehalase (microbial, blowfly, mammal), not inhibits glucoside hydrolases (alpha-, beta-glucosidase or glucoamylase).

本课题研究的海藻糖酶抑制剂是由本课题组分离得到的一种新型海藻糖酶抑制剂--海藻糖抑制素（Trehalostatin），其分子式经二次<13><22><10>西姆斯质谱（m/z 367，[M+H]^+）和元素分析确定为C_ H_ O_ N_2。根据各种光谱数据（13 C-，1H-，H，H-COSY，C，H-COSY，NOESY光谱），结构被阐明为假二糖，由α-D-吡喃葡萄糖苷和5元环醇部分组成。证明了两个部分之间的键合是异脲基键。海藻糖抑制素特异性抑制多种海藻糖酶（微生物、丽蝇、哺乳动物），而不抑制糖苷水解酶（α-、β-葡萄糖苷酶或葡糖淀粉酶）。

项目成果

Sawao Murao: "A Novel Trehalase Inhibitor,Trehalostatin,Produced by Amycolatopsis trehalostatica HGー25" Agric.Biol.Chem.,. 55. 895-897 (1991)

Sawao Murao：“一种新型海藻糖酶抑制剂，Trehalostatin，由 Amycolatopsis trehalostatica HG-25 生产”，Agric.Biol.Chem., 55. 895-897 (1991)

Sawao Murao: "A Novel Trehalase Inhibitor,Trehalostatin,Produced by Amycolatopsis trehalostatica HGー25" Agric.Biol.Chem.55. 895-897 (1991)

Sawao Murao：“一种新型海藻糖酶抑制剂，Trehalostatin，由 Amycolatopsis trehalostatica HG-25 生产”Agric.Biol.Chem.55 (1991)。

Sawao Murao et al: "Structural Elucidation of Novel Trehalase Inhibitor, Trehalosatin" J. Amer. Chem. Soci.

Sawao Murao 等人：“新型海藻糖酶抑制剂海藻糖素的结构阐明”J. Amer。

Sawao Murao et al: "A Novel Trehalase Inhibitor, Trehalostatin, Produced by Amycolatopsis trehalosatica HG-25." Agric. Biol. Chem.55(3). 895 (1991)

Sawao Murao 等人：“一种新型海藻糖酶抑制剂，海藻抑素，由 Amycolatopsis trehalosatica HG-25 生产。”

S.Murao: "A Novel Trehalase Inhibitor<Trehalostatin<Produced by Amycolatopsis trehalostatica" Agric.Biol.Chem.

S.Murao：“一种新型海藻糖酶抑制剂<Trehalostatin<由Amycolatopsis trehalostatica生产”Agric.Biol.Chem。