Studies on Trehalase Inhibitor

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The project was concerned on the novel trehalase inhibitor, Trehalostatin, produced by Amycolatopsis trehalostatica HG-25, which was isolated by the investigators.The molecular formula was determined to be C_<13>H_<22>O_<10>N_2 by SIMS spectrum (m/z 367, [M+H]^+) and elemental analysis. From various spectroscopic data (13C-, 1H-, H, H-COSY, C, H-COSY, NOESY spectra), the structure was elucidated to be an pseudo-disacchaide, composed of alpha-D-glucopyranoside and 5-membered cyclitol moieties. The linkage of the two moieties was proved to be isoureido bond. This structure was a novel one, not yet reported.Trehalostatin specifically inhibits various trehalase (microbial, blowfly, mammal), not inhibits glucoside hydrolases (alpha-, beta-glucosidase or glucoamylase).