THE ROLE OF TREHALOSE IN INSECT REPRODUCTION AND THE APPLICATION OF TREHALASE INHIBITOR FOR PEST MANAGEMENT.

海藻糖在昆虫繁殖中的作用以及海藻糖酶抑制剂在害虫防治中的应用。

• 批准号: 08456033
• 负责人: KONO Yoshiaki
• 金额: $ 4.67万
• 依托单位: NATIONAL INSTITUTE OF INFECTIOUS DISEASES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456033/
• 关键词: TREHALOSE; TREHALASE INHIBITOR; VALIDOXYLAMINE A; COCKROACH; GRASS HOPPER; FLIGHT; REPRODUCTION; INSECTICIDAL ACTIVITY; ワモンゴキブリ; トノサマバッタ; アブラムシ; 卵発育; ビテロシェニン

Injection of a specific and potent trehalase inhibitor, validoxylamine A (VAA), suppressed oocyte development in the American cockroach, Periplaneta americana and the smoky brown cockroach, Periplaneta fuliginosa. Ootheca formation was also affected by the same treatment. However, the suppressive activity of VAA on the reproduction was not so strong in the German cockroach, Blattella germanica. Trehalases of ovaries, fat body and accessary gland of P.americana were strongly inhibited by the injection of VAA.In control cockroaches, vitellin accumulated in the ovary in parallel with oocyte development, and amount of vitellogenin in the hemolymph changed showing negative correlation with intake of vitellogenin by the ovary. In injected cockroaches, vitellogenin production was suppressed in the early stage of oocyte development, and intake of vitellogenin by the ovary reduced in the late stage and consequently vitellogenin accumulated in the hemolymph. It is concluded that the primary effect of VAA is on the vitellogenin production and intake for the oocyte development.In the migratory locust, Locusta migratoria, VAA also suppressed oocyte development when injected to reproductively active females. Most individuals injected with VAA stopped oocyte development completely after producing a few egg-pods, whereas water-injected controls continued to lay eggs until they died. VAA did not influence longevity of females unless a large dose (100 ug/animal) was given. VAA suppressed vitellogenin synthesis by the fat body, and uptake of yolk material by the ovary of the locust as in the cockroach. Furthermore, the juvenile hormone synthesis in corpora cardiaca was suppressed.VAA showed suppression of the ootheca formation of P.americana when administered to the male indicating its inhibitory effect on the fertilization.These results indicate that trehalase inhibitors would be useful for the pest control by inhibiting the reproduction of insects.

注射一种特异性和有效的海藻糖酶抑制剂，validoxylamine A（VAA），抑制卵母细胞发育的美洲大蠊，美洲大蠊和烟棕色蟑螂，黑胸大蠊。卵鞘的形成也受到相同处理的影响。而VAA对德国小蠊的生殖抑制作用不明显。注射VAA后，美洲大蠊卵巢、脂肪体和附属腺的海藻糖酶活性受到强烈抑制，对照组卵巢中卵黄蛋白的积累与卵母细胞的发育同步，血淋巴中卵黄蛋白原的含量变化与卵巢对卵黄蛋白原的摄取呈负相关。在注射蟑螂，卵黄蛋白原的生产受到抑制，在卵母细胞发育的早期阶段，并通过卵巢的卵黄蛋白原的摄入减少在后期阶段，因此卵黄蛋白原积累在血淋巴中。VAA对卵母细胞发育的影响主要是影响卵母细胞对卵黄蛋白原的合成和摄取，对繁殖活跃的雌性飞蝗（Locusta migratoria）也有抑制作用。大多数注射VAA的个体在产生几个卵荚后完全停止卵母细胞发育，而注射水的对照组继续产卵直到死亡。除非给予大剂量（100微克/只动物），否则VAA不会影响雌性动物的寿命。VAA抑制卵黄蛋白原合成的脂肪体，和吸收的蛋黄材料的卵巢蝗虫在蟑螂。此外，VAA还抑制了心体中保幼激素的合成，VAA对美洲大蠊的卵囊形成有抑制作用，表明其对美洲大蠊的受精有抑制作用，这些结果表明海藻糖酶抑制剂可能通过抑制昆虫的繁殖来防治害虫。

项目成果

Kono,Y.et al.: "In vitro trehalose synthesis in American cockroach fat body measured by NMR spectroscopy using ^<13>C-sugars and a trehalase inhibitor,validoxylamine A." Med.Entomol.Zool.49. 321-330 (1998)

Kono,Y.等人：“使用 13 C-糖和海藻糖酶抑制剂有效羟胺 A，通过 NMR 光谱测量美洲大蠊脂肪体中的体外海藻糖合成。”

Y.Kono et al.: "Effect of a trlhalase inhibitor,validexylamineA,on cooyte development and ootheca formation in Periplapcta americana." Appl.Entomol.Zool.32(2). 293-301 (1997)

Y.Kono 等人：“三糖酶抑制剂validexylamineA 对美洲周幼虫幼狼发育和卵鞘形成的影响”。

M.Takahashi, Y.Kono, H.Kurahashi, K.Matsushita, M.Nishina and Y.Kameda: "Effect of a trehalase inhibitor, validoxylamine A,on three species of flies." Appl.Entomol.Zool.30. 231-139 (1995)

M.Takahashi、Y.Kono、H.Kurahashi、K.Matsushita、M.Nishina 和 Y.Kameda：“海藻糖酶抑制剂有效木胺 A 对三种果蝇的影响。”

Y.Kono, M.Takahashi, M.Mihara, K.Matsushita, M.Nishina and Y.Kameda: "Effect of a trehalase inhibitor, validoxylamine A,on oocyte development and ootheca formation in Periplaneta americana." Appl.Entomol.Zool.32. 293-301 (1997)

Y.Kono、M.Takahashi、M.Mihara、K.Matsushita、M.Nishina 和 Y.Kameda：“海藻糖酶抑制剂有效羟胺 A 对美洲大蠊卵母细胞发育和卵鞘形成的影响”。

Takahashi,M.et al.: "Effect of a trehalase inhibitor,validoxylamine A,on three species of flies." Appl.Entomol.Zool.30. 231-239 (1995)

Takahashi,M.et al.：“海藻糖酶抑制剂，validoxylamine A，对三种果蝇的影响。”