High Degree Utilization of Leaf Fstorides and Their Degradative products, omega -Hydroxyacids, from Main Japanese Conifers

日本主要针叶树叶化合物及其降解产物欧米茄羟基酸的高度利用

• 批准号: 01560180
• 负责人: OHASHI Hideo
• 金额: $ 1.41万
• 依托单位: Gifu University, Faculty of Agriculture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560180/
• 关键词: Plant resources; Conifer; Needle-leaf; Estoride; omega-Hydroxyacid; 10, 16-Dihydroxyhexadecanoic acid; Aliphatic alcohol; オメガオキシ酸; 爆砕; セルラ-ゼ; ペクチナ-ゼ; 10、16-ジヒドロキシヘキサデカン酸; サビニン酸; ジュニペリン酸; 7-ヒドロキシヘキサデカン-1、16-ジカルボン酸

For the purpose of development of utilization methods on plant resources, the investigator attended to Japanese conifer leaves which had not been used for the human life. Together with carbohydrates such as cellulose and polyphenols such as lignin, the leaf contains a large amount of cutin constituent. Cutin is a mixture of oligo-esters, estoride, composing from aliphatic acids and alcohols. The study was undertaken in order to establish the direct and indirect utilization methods of leaf estorides.At first, total estoride contents were estimated on 23 species of conifer leaves. Extract-free leaf powders respectively were analyzed by Klason and acetyl bromide methods which were common to quantitative analysis of lignin. Then, it was evaluated that the content difference between two values meant a real estoride content. The values among samples were covered a limit of 10-20 % for the dried leaf powder weight.Nextly, alkaline hydrolyses of 42 samples were undergone, and their total acid … More and alcohol contents were obtained respectively. The former values ranged from 1 % to 6 %, and the latter comprised from trace to 14 % against the corresponding acid content. Also, the identification arid quantitative analysis of acids and alcohols were carried out. Nine acids containing 12-hydroxylauric acid, 10, 16-dihydroxyhexadecanoic acid, hexadecane-1, 16-dioic acid, etc. were identified and their qualitative and quantitative relations were proved. The presence pattern among samples was characteristic in the plant species. Similarly, six alcohols such as 1, 12-dodecandiol, 1, 14-tetrade-canediol, 1, 16-hexadecanediol were isolated and measured. The presence tendency among samples was similar to that of the acids.As there was a large gap between the estoride content and the calculated content based on the hydrolized acid and alcohol amounts, Various pretreatment procedures were attempted for the effective possession of estoride and its degradative products. Accordingly, steam-explosion, chlorine and enzyme treatments were effective for this purpose. Furthermore, the changes due to leaf age and season were examined on estoride and its composing monomer contents. It was indicated there were not remarkable qualitative and quantitative differences among samples.Consequently, for the most effective possession of some objective estoride and/or monomer, one suitable sample and pretreatment method can be presented. Then, it can be judged that these knowledges is available for developing utilization methods of leaf estoride. Less

为了开发植物资源的利用方法，调查者注意到了尚未用于人类生活的日本针叶。与纤维素等碳水化合物和木质素等多酚一起，树叶中含有大量角质成分。角质是由脂肪酸和醇组成的寡酯和雌酮的混合物。为了建立叶片雌激素的直接和间接利用方法，本研究首先对23种针叶树叶中雌激素的总含量进行了测定。采用木质素定量分析中常用的Klason法和乙酰溴化法分别对无抽提物的叶粉进行了分析。然后，评估两个值之间的含量差异意味着实际的雌酮含量。其次，对42个样品进行了碱解，测定了它们的总酸…分别获得了较高的酒精度和较高的酒精含量。前者的取值范围为1%~6%，后者的取值范围为痕量~14%。并对酸类和醇类进行了鉴定和定量分析。鉴定出含有12-羟基月桂酸、10，16-二羟基十六酸、十六烷-1，16-二元酸等9种酸，并证明了它们之间的定性和定量关系。样品之间的存在模式是植物物种特有的。同样，分离并测定了1，12-十二二醇、1，14-十四碳二醇、1，16-十六碳二醇等六种醇类化合物。样品之间的存在趋势与酸的存在趋势相似，由于依托利的含量与根据氢化酸和乙醇的量计算的含量存在较大差距，因此尝试了不同的前处理程序，以有效地拥有依托利及其降解产物。因此，蒸汽爆破、氯气和酶处理对此目的是有效的。此外，还考察了叶龄和季节对雌酮及其组成单体含量的影响。结果表明，样品之间没有显著的定性和定量差异。因此，为了最有效地掌握某些客观的雌酮和/或单体，可以提供一种合适的样品和前处理方法。因此，可以判断这些知识可用于开发叶片雌雄激素的利用方法。较少

项目成果

大橋 英雄,他: "日本産針葉樹葉のクチンエストライド及び構成モノマ-" Folzforshung.

Hideo Ohashi 等人：“日本针叶叶的角质酯和组成单体”Folzforshung。

大橋英雄: "日本産針葉樹葉中のエストライド量とその構成オメガオキシ酸"

Hideo Ohashi：“日本针叶树叶子中的雌激素含量及其欧米伽含氧酸的组成”

Hideo Ohashi et al.: "Estorides and Their Composing Monomers from Leaf Cutins of Japanese Conifers" Folzfolshung.

Hideo Ohashi 等人：“日本针叶树叶角质中的 Estorides 及其组成单体”Folzfolshung。

大橋 英雄・他: "日本産針葉樹葉のクチンエストライド及びその構成モノマ-" Folzforschung.

Hideo Ohashi 等人：“来自日本针叶叶的 Cutinestride 及其组成单体”Folzforschung。

大橋 英雄・他: "日本産針葉樹葉中のエストライド量とその構成オメガヒドロキシ酸類について"

Hideo Ohashi 等人：“关于日本针叶树叶子中 estride 的含量及其 omega 羟基酸的组成”