Establishment of mass acquisition methods of cutinand its hydrolyzed products from coniferous leaves, and trials on their higher utilization methods

针叶叶角质及其水解产物的大量获取方法的建立及其更高利用率的试验

• 批准号: 07556101
• 负责人: OHASHI Hideo
• 金额: $ 3.84万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556101/
• 关键词: Coniferous leaves; Cutin; Cutin monomers; Propyleneglycol treatment; polyethyleneglycol treatment; Solubilization of wood; alkaline hydrolysis; omega-hydroxyfatty acid; 硫酸処理; 酵素処理; バイオマス; 針葉樹; 葉成分; エストライド; 大量取得; 高度利用法; 10,16-ジヒドロキシヘキサデカン酸

The present work was carried out in order to establish the effective utilization methods of leaf and twigs which account for about 50 % of the whole coniferous tree. Especially, cutin of Japanese cedar and cypress leaf cells was noted as the target compound for the effective utilization. The results on the last year of this research are as follows.1) The quantitative analysis method for total cutin was established and cutin amounts of main Japanese coniferous tree leaves were determined.2) Monomers and their quantities were determined after alkaline hydrolysis of cutin from main Japanese coniferous tree leaves.3) Quantitative analysis of alkaline hydrolysis products of cutin was undertaken with regard to samapling season, leaf age, mixing of leaf, rachis and twig, and so on, and these results assured the researchers for safety in carring out the development.4) For the purpose of mass aquisition of cutin and its hydrolized compounds, some treatment methods were studied. (1) The steam-ex … More plosion treatment was good method forobtaining of cutin monomers. (2) The effect of enzyme treatment with cellulase and/or pectinase was examined, though there was the economic problem. (3) The examination on propyleneglycol treatment was revealed that this treatment was usuful on the mass aquisition of cutin together with cellulose, after removal of lignin. (4) The treatment with polyethyleneglycol in the presence of a trace of H_2SO_4 solubilied main leaf components except cutin, that is, cutin was concentrated as insoluble materials. The solubilized main leaf components could be adapted the preparation of foamed plastic. (5) The treatments with p-cresol-H_2SO_4 enabled the utilization of lignin, in addition to concentration of cutin. (6) Removal treatments with H_2SO_4 and trifluoroacetic acid against the materials obtained from the above treatments were more effective on the purification of cutin.5) The basic knowledges on the gelation of 10,16-dihydroxyhexadecanoic acid was obtained for the development of gelling materials. Less

本工作的目的是建立约占整棵针叶树50%的叶和枝的有效利用方法。特别是，日本柳杉和柏树叶细胞的角质被认为是有效利用的目标化合物。本研究去年的研究成果如下：1）建立了日本主要针叶树叶片总角质的定量分析方法，测定了日本主要针叶树叶片的角质量。2）测定了日本主要针叶树叶片角质碱解后的单体及其含量。3）从取样季节、叶龄、叶与轴的混合情况等方面对角质碱解产物进行了定量分析。 4)为了大量获得角质及其水解物，研究了一些处理方法。 (1)蒸汽爆炸处理是获得角质单体的良好方法。 (2)尽管存在经济问题，但仍检查了用纤维素酶和/或果胶酶进行酶处理的效果。 (3)对丙二醇处理的检验表明，该处理对于在去除木质素后大量获得角质和纤维素是有用的。 (4)在微量H_2SO_4存在下用聚乙二醇处理可溶解除角质以外的叶片主要成分,即角质被浓缩为不溶物。溶解的主要叶成分可用于制备泡沫塑料。 (5)对甲酚-H_2SO_4处理除了可以浓缩角质外,还可以充分利用木质素。 (6)对上述处理所得材料进行H_2SO_4和三氟乙酸去除处理,对角质的纯化效果更佳。5)获得10,16-二羟基十六烷酸凝胶化的基础知识,为凝胶材料的开发奠定基础。较少的