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Patch-clamp studies on the membrane properties of granulocytes and their changes during cell-activation.

关于粒细胞膜特性及其在细胞激活过程中变化的膜片钳研究。

基本信息

批准号：
01570038
负责人：
KAWA Kazuyoshi
金额：
$ 1.34万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570038/
关键词：
Patch-clamp Neutrophil K^+ -channel Granulocytes Endocrine cells イオンチャネル

项目摘要

The blood obtained from the atria of the newts (Triturus pyrrhogaster) contains several types of leukocytes. Under a phase contrast microscope, three types of granulocytes could be identified. Using patch-electrodes, it was possible to investigate the whole-cell membrane currents in these cells. In the neutrophils (diameter, 19-23 mum), identified by characteristic features of lobulated nucleus, inward rectifying K^+ currents were evoked with hyperpolarization from the holding potential of -83 mV. In the presence of Cs^+ (1 mM) or Ba^<2+> (1 mM) in the external saline, the amplitudes of inward currents were reduced with time- and voltage- dependent manner. When the external concentration of K^+ was increased from 4 mM through 20 mM to 100 mM replacing isomolar choline in the perfusing salines, the threshold of the K^+ currents shifted from -83 mV through -43 mV to -3 mV.The amplitude of the evoked K^+ currents also increased as the chord conductance in each of these salines was nearly proportional to the square root of the external K^+ concentration.In basophils and eosinophils, there evoked no major membrane currents under voltage-clamp conditions. Their I-V relations were almost linear, which suggested almost electrically non-excitable properties in these cells. These electrophysiological properties of granulocytes were compared with those of endocrine cells.

从蝾螈（Triturus pyrrhogaster）心房获得的血液含有几种类型的白细胞。在相差显微镜下，可以识别出三种类型的粒细胞。使用贴片电极，这是可能的，调查在这些细胞的全细胞膜电流。在中性粒细胞（直径19-23 μ m）中，通过分叶核的特征识别，从-83 mV的保持电位超极化诱发内向整流K^+电流。在外加Cs^+（1 mM）或Ba^<2+>（1 mM）的盐水溶液中，内向电流的幅度呈时间和电压依赖性降低。当K^+的外部浓度从4 mM增加到20 mM，再增加到100 mM时，在灌注盐水中替换等摩尔的胆碱，K^+电流的阈值从-83 mV通过-43 mV移动到-3 mV。诱发的K^+电流的幅度也随着这些盐水中的腱电导几乎与外部K^+的平方根成正比而增加在嗜碱性粒细胞和嗜酸性粒细胞中，在电压钳条件下没有引起主要的膜电流。它们的I-V关系几乎是线性的，这表明在这些细胞中几乎是电不可兴奋的性质。将粒细胞的这些电生理特性与内分泌细胞的电生理特性进行了比较。