Natural Life Cycle of Gnathostoma Doloresi in Miyazaki Prefecture, with Emphasis on the Route of Infection to the Patients.

1988年，我们在宫崎县发现了首例人间口吸虫感染确诊病例。此外，通过对7例疑似病例的石蜡包埋皮肤进行回顾性调查，又发现2例确诊病例。截至目前，宫崎县中部共发现人间口吸虫病病例14例，其中确诊病例4例。在这14例病例中，13例是垂钓者或其妻子有共同的既往进食生淡水鱼切片肉的病史，主要是Oncorhynchus Masou。有一例患者过去有吃过蛇的生肉片的病史。根据患者的病史，我们进行了一项调查，以阐明多洛雷斯氏菌的自然生活史，并确定患者的感染源。在宫崎县山区捕获的野猪Sus scrofa leucomystax。感染多洛莱斯革兰氏菌，发病率为100%。宫内卵子…人工孵化L 1号在第一中间宿主中，更多的成虫雌虫可长成L_3。冷水独眼巨人。作为第二中间宿主和/或副宿主，毒蛇是在宫崎县山区捕获的。感染L 3号革兰氏菌，发病率为100%。从弓形虫中回收的L_3经实验感染猪可发育成成熟成虫。此外，无毒蛇Elaphe furivergata也含有多洛雷斯氏原虫L_3。这些结果表明，蛇在该寄生虫的自然生活史中起着重要作用。从患者的情况来看，淡水鱼最有可能是患者的感染源。因此，我们对两种淡水鱼进行了检测，日语中常见的名称为“Yamame”的Oncorhynchus Masou和常见的名称为“蓝鳃”的Lepomis macchirus，这两种鱼都是在Hitotsuse河捕获的。虽然每条鱼都检查了200多条，但还没有从这些鱼中发现多洛雷西螺幼虫。有必要进行进一步的广泛调查，以查明感染源。较少

In 1988, we found the first confirmed human case of Gnathostoma doloresi infection in Miyazaki Prefecture. In addition, two more confirmed cases were found by the retrospective survey of paraffin-embedded biopsied skin of 7 suspected cases. Up to now, a total of 14 human gnathostomiasis doloresi cases including 4 confirmed cases were found in the central part of Miyazaki Prefecture. Among these 14 cases, 13 cases were anglers or their wife having a common past history of eating raw slice meat of freshwater fishes, mainly Oncorhynchus masou. One case had a past history of eating raw slice meat meat of a snake, Agkistrodon halys. Based on the history of the patients, we conducted a survey to elucidate the natural life cycle of G. doloresi and to identify the source of infection to the patients. The wild boars, Sus scrofa leucomystax, captured in the mountainous areas of Miyazaki Pref. Were infected with G. doloresi with 100% incidence. Artificially hatched L_1 from the intrauterine eggs … More from the adult female worms could grew up into L_3 in the first intermediate host. cold water cyclops. As the second intermediate host and/or the paratenic host, the poisonous snakes, Agkistrodon halys, captured in the mountainous areas of Miyazaki Pref. were infected with G. doloresi L_3 with 100% incidence. The L_3 recovered from A. halys could develop into mature adult worms by an experimental infection in a pig. In addition, a nonpoisonous snake, Elaphe quadrivergata, also harbored G. doloresi L_3. These results suggest that snakes are important in the natural life cycle of this parasite. From the anamnes is of the patients, freshwater fishes are most likely source of infection to the patients. Therefore, we examined two kinds of freshwater fishes, common Japanese name "Yamame", Oncorhynchus masou, and common name "Blue gill" , Lepomis macrochirus, both captured at Hitotsuse River. Although more than 200 each were examined, G. doloresi larvae were not yet found from these fishes. Further extensive survey is necessary for the identification of the source of infection. Less