Studies on the effects of ADP-ribosyltransferase activities of staphylococcal cytolytic toxins on the signal transduction in cell membrane.

葡萄球菌溶细胞毒素ADP-核糖基转移酶活性对细胞膜信号转导影响的研究。

• 批准号: 01570228
• 负责人: NODA Masatoshi
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570228/
• 关键词: Staphylococcal alpha-toxin; Leukocidin S and F comp.; ADP-ribosyltransferase; Phospholipase A_2; Phospholipase C; GTP-binding protein; Protein kinase C; PI代謝; PIキナ-ゼ; PIPキナ-ゼ; ADPリボシルトランスフェラ-ゼ; ホスホリパ-ゼA_2; ホスファチジルイノシト-ル代謝系

1. Staphylococcal alpha-toxin resulted in ADP-ribosylation of the 37 and 41 kDa proteins of a membrane preparation from rabbit erythrocytes. In the presence of 100 muM GTP, the toxin ADP-ribosylated proteins of 54 and 59 kDa and potentiated ADP-ribosylation of the 37 and 41 kDa forms. GTP had no effect on ADP-ribosylation of membrane proteins in the absence of alpha-toxin.2. Incubation of a membrane preparation of rabbit polymorphonuclear leukocytes with the S and F components of staphylococcal leukocidin resulted in ADP-ribosylation of the 37 and 41 kDa proteins, respectively. Furthermore, the 37, 41, 54 and 59 kDa proteins were ADP-ribosylated by leukocidin in the presence of GTP.3. The ADP-ribosylation of these proteins was observed to be dependent on the incubation time and toxin dose and was abolished by prior boiling. Addition of agmatine did not attenuate ADP-ribosylation of these proteins.4. The S component of staphylococcal leukocidin ADP-ribosylated 37 kDa GTP-binding protein involved in the transduction to activate phospholipase A_<2'> and the F component resulted in ADP-ribosylation of 41 kDa GTP-binding protein involved in the transduction to activate phosphoinositide-specific phospholipase C.

1. 葡萄球菌α毒素导致兔红细胞制备膜的37和41 kDa蛋白的adp核糖基化。在100mum GTP存在下，毒素使54和59 kDa的adp核糖基化，并增强了37和41 kDa形式的adp核糖基化。在不含α -毒素的情况下，GTP对膜蛋白adp -核糖基化无影响。用葡萄球菌杀白细胞素的S和F组分培养兔多形核白细胞的膜制剂，分别导致37和41 kDa蛋白的adp核糖基化。此外，在gtp存在的情况下，37、41、54和59 kDa蛋白被杀白细胞素adp核糖化。观察到这些蛋白的adp核糖基化依赖于孵育时间和毒素剂量，并通过事先煮沸消除。添加agmatine并没有减弱这些蛋白的adp核糖基化。葡萄球菌嗜白素的S组分adp -核糖化37 kDa的gtp结合蛋白参与转导激活磷脂酶A_<2'>， F组分导致41 kDa的gtp结合蛋白adp -核糖化参与转导激活磷酸肌醇特异性磷脂酶C。

项目成果

王 夏、野田 公俊,加藤 巌: "黄色ブドウ球菌ロイコシジンのPI kinaseに及ぼす影響" 臨床と微生物. 17. 220-220 (1990)

Xia Wang、Kimitoshi Noda、Iwao Kato：“金黄色葡萄球菌杀白细胞素对 PI 激酶的影响”临床和微生物学 17. 220-220 (1990)。

宇井 理生、高井 義美、堅田 利明 編、野田 公俊、加藤 巌: "羊土社(東京)" GTP結合蛋白質と情報伝達,

Rio Ui、Yoshimi Takai、Toshiaki Katata（编辑）、Kimitoshi Noda、Iwao Kato：“Yodosha（东京）”GTP 结合蛋白和信息传输，

越部 融、加藤 巌,野田 公俊、高橋 英世: "黄色ブドウ球菌α毒素のホスホリパ-ゼA_2およびシクロオキシゲナ-ゼ活性に及ぼす影響" 医学と生物学. 122. (1991)

Toru Koshibe、Iwao Kato、Kimitoshi Noda、Hideyo Takahashi：“金黄色葡萄球菌 α 毒素对磷脂酶 A_2 和环氧合酶活性的影响”医学和生物学 122。（1991）

Kato, I., Noda, M.: "ADP-ribosylation of cell membrane proteins by staphylococcal alpha-toxin and leukocidin in rabbit erythrocytes and polymorphonuclear leukocytes." FEBS Letter. 225. 59-62 (1989)

Kato, I.，Noda, M.：“兔红细胞和多形核白细胞中葡萄球菌 α 毒素和杀白细胞素对细胞膜蛋白的 ADP 核糖基化。”

Noda, M., Kato, I.: "GTP-binding proteins and signal transduction." Experimental Medicine. vol. 8. 260-264 (1990)

Noda, M.，Kato, I.：“GTP 结合蛋白和信号转导。”