权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Serological Diagnosis and Seroepidemiological Study of Q Fever.

Q热的血清学诊断和血清流行病学研究。

基本信息

批准号：
01570240
负责人：
ODA Hiroshi
金额：
$ 0.32万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

1. The growth of Q fever agent (Coxiella burnetii) in vivo and in vitro : Treatment of mice with cyclophosphamide facilitated the growth of Coxiella burnetii so effectively that isolation of the organism became easier. The growth of this rickettsia in cultured cells differed markedly according to the type of cells and the species of animals from which the cells derived.2. Preparation of antigens for serological diagnosis of Q fever : Coxiella burnetii, strain TK-1, in phase I propagated well in yolk sacs of embryonated chicken eggs, and we could obtain enough antigen for serological tests. Phase II antigen of Coxiella burnetii was tried to make by serial passage in yolk sac, and we attained to the 20th passage. On the other hand, trichloroacetic acid-treated phase I antigen reacted strongly with anti-phase II antibody. Therefore this chemically modified antigen must be useful for serological diagnosis.3. Serological diagnosis of Q fever : Immunofluorescence technique was adequate for laboratory examination of Q fever from the viewpoints of rapidity, sensitivity and practicality. Western immunoblotting method was also adequate to check the specificity of the antibody against Coxiella burnetii in reference to 60 kilodalton common antigen.4. Seroepidemiological study of Q fever : Serological examination of bovine and human sera for antibodies against Coxiella burnetii was carried out by the immunofluorescence technique. About 30% of the cows examined were antibody-positive. Sera from two veterinarians also had antibody against C. burnetii. These results suggest that the Q fever agent has spread widely in Japan. Therefore Q fever must be added to the list of differential diagnosis of unknown fever.

1.Q热剂(贝氏柯克斯体内外)的生长：用环磷酰胺处理小鼠，能有效地促进贝氏柯克斯体的生长，使其更容易分离。这种立克次体在培养细胞中的生长因细胞类型和细胞来源的动物种类的不同而明显不同。Q热血清学诊断抗原的制备：伯氏柯克斯体TK-1株，I期在鸡胚卵黄囊内繁殖良好，可获得足够的抗原用于血清学检测。尝试在卵黄囊内连续传代制备伯氏柯克斯体II相抗原，传代至第20代。另一方面，经三氯乙酸处理的I相抗原与抗II相抗体反应强烈。因此，这种化学修饰的抗原对血清学诊断一定是有用的。Q热的血清学诊断：从快速、灵敏、实用的角度来看，免疫荧光技术适合于Q热的实验室检测。以60kodalton共同抗原为参照物，免疫印迹法也能较好地检测该抗体的特异性。Q热病血清流行病学研究：用免疫荧光技术对牛和人血清进行贝氏柯克斯体抗体检测。接受检查的奶牛中约有30%的抗体呈阳性。两名兽医的血清中也有抗伯氏梭菌的抗体。这些结果表明Q热剂已在日本广泛传播。因此，Q热必须添加到不明原因发热的鉴别诊断名单中。