Attempt to develop a simple and rapid method for diagnosis of cat scratch disease (Bartonella infection).
尝试开发一种简单快速的方法来诊断猫抓病(巴尔通体感染)。
基本信息
- 批准号:15590393
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1.Analysis by SDS-PAGE of whole bacterial cells of Bartonella henselae revealed more than 35 protein bands. The molecular weight of major proteins were 90, 62, 45 (43), 40, 35, 31, and 25kDa. Molecular weight of the major outer membrane proteins were estimated as 62, 43 and 31kDa.2.Thirty-two of 108 patients who had swelling of lymph nodes and CSD was suspected showed more than 256x IgG titer against B.henselae by indirect immunofluorescence assay (IFA). These antibody positive sera were used for the following study.3.Western blotting analysis of whole bacterial cells of B henselae using patient sera revealed 8 bands (90, 65, 43, 40, 35, 33, 30, and 28kDa). Distribution pattern of positive bands were diverse in case to case, however, 65, 43, and 35kDa proteins showed strong antigenicity. The reactivity of IFA increased by co-cultivation of B.henselae with vero cells. This increase of reactivity were striking in 35kDa band, however, some sera did not reacted with this band. Consequently we could not find sole antigen protein useful for sero-diagnosis. Compound proteins, including 65, 43, and 35kDa protein, should be considered to develop new method for the laboratory diagnosis of CSD.4.B.henselae stimulated the growth of lymphatic endothelial cells as well as vascular endothelial cells. B.henselae induced CCR7 on the surface of peripheral neutrophils. The bacteria also stimulated the production of CCL21 from lymphatic endothelial cells. Then we examined the diagnostic importance of CCL21 in patients with CSD. The result showed no significant difference of serum CCL21 level between CSD and other patients with various lymph node diseases.5.We developed a new method to cultivate B.henselae, and isolated 16 strains of B.henselae from stray cats by using the method.
1.对汉赛巴尔通体全菌进行SDS-PAGE分析,发现其蛋白质条带超过35条。主要蛋白质的分子量为90、62、45(43)、40、35、31和25 kDa。2.间接免疫荧光法(IFA)检测108例淋巴结肿大疑似CSD患者中32例抗汉赛B IgG效价> 256 ×。3.汉赛B全菌蛋白免疫印迹分析显示8条带(90、65、43、40、35、33、30和28 kDa)。阳性条带的分布方式在不同病例中有差异,但65、43和35 kDa蛋白具有较强的抗原性。汉赛B.henselae与vero细胞共培养可提高IFA的反应性。这种反应性的增加在35 kDa条带中是显著的,然而,一些血清不与该条带反应。因此,我们不能找到唯一的抗原蛋白用于血清学诊断。因此,应考虑利用65、43和35 kDa的复合蛋白作为CSD的实验室诊断新方法。4.汉赛布氏杆菌能刺激淋巴管内皮细胞和血管内皮细胞的生长。汉赛B.henselae诱导外周中性粒细胞表面的CCR 7。该细菌还刺激淋巴管内皮细胞产生CCL 21。然后,我们研究了CCL 21在CSD患者中的诊断重要性。结果表明,CSD患者血清CCL 21水平与其他淋巴结疾病患者无显著性差异。5.建立了一种新的汉赛B.henselae的培养方法,并利用该方法从流浪猫中分离到16株汉赛B.
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis.
牙龈卟啉单胞菌的热稳定成分诱导人脐静脉内皮细胞上的细胞间粘附分子-1。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Ken-ichi Ishibashi;Yukari Nakagawa;Naohito Ohno;Toshimi Murai;Yoshiyuki Adachi;Noriko N. Miura;Naohito Ohno;Song Mao
- 通讯作者:Song Mao
Increased interleukin-18 expression in bone marrow of a patient with systemic juvenile idiopathic arthritis and unrecognized macrophage-activation syndrome.
患有全身性幼年特发性关节炎和未识别的巨噬细胞激活综合征的患者骨髓中白细胞介素 18 的表达增加。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:N. Maeno;S. Takei;H. Imanaka;Kimie Yamamoto;K. Kuriwaki;Y. Kawano;H. Oda
- 通讯作者:H. Oda
The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by heat-stable component of Porphyromonas gingivalis
牙龈卟啉单胞菌热稳定成分对人脐静脉内皮细胞细胞间粘附分子1的诱导
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Song Mao;et al.
- 通讯作者:et al.
Antiagalactosyl IgG Antibodies in juvenile idiopathic arthritis, juvenile onset Sjogren's syndrome, and healthy children.
抗半乳糖基 IgG 抗体用于青少年特发性关节炎、青少年发病的干燥综合征和健康儿童。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Nobuaki Maeno;Syuji Takei;Hiroshi Oda;他6名
- 通讯作者:他6名
Mao S (毛 松): "The induction of intercellular adhesion molecule-l on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis."Current Microbiology. 48(2). 108-112 (2004)
Mao S:“牙龈卟啉单胞菌的热稳定成分对人脐静脉内皮细胞的细胞间粘附分子-1的诱导”,《当代微生物学》48(2)108-112(2004)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
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ODA Hiroshi其他文献
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{{ truncateString('ODA Hiroshi', 18)}}的其他基金
Visible and near-infrared multicolor photometry using a small-aperture telescope with wide field of view: Space weathering of near-Earth objects
使用大视场小口径望远镜进行可见光和近红外多色光度测量:近地天体的空间风化
- 批准号:
15K21678 - 财政年份:2015
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
An anthropological study of postcolonial reconciliation: the case study of relations between Germany and its former African colonies
后殖民和解的人类学研究:德国与其前非洲殖民地关系的案例研究
- 批准号:
22520813 - 财政年份:2010
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Isolation and characterization of endothelial cell growth factor from Bartonella henselae
汉赛巴尔通体内皮细胞生长因子的分离和表征
- 批准号:
12670259 - 财政年份:2000
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanism of growth stimulatory effect of vascular endothelial cells by Bartonella henselae.
汉赛巴尔通体刺激血管内皮细胞生长的机制。
- 批准号:
09670290 - 财政年份:1997
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Serological Diagnosis and Seroepidemiological Study of Q Fever.
Q热的血清学诊断和血清流行病学研究。
- 批准号:
01570240 - 财政年份:1989
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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