Attempt to develop a simple and rapid method for diagnosis of cat scratch disease (Bartonella infection).

1.对汉赛巴尔通体全菌进行SDS-PAGE分析，发现其蛋白质条带超过35条。主要蛋白质的分子量为90、62、45（43）、40、35、31和25 kDa。2.间接免疫荧光法（IFA）检测108例淋巴结肿大疑似CSD患者中32例抗汉赛B IgG效价> 256 ×。3.汉赛B全菌蛋白免疫印迹分析显示8条带（90、65、43、40、35、33、30和28 kDa）。阳性条带的分布方式在不同病例中有差异，但65、43和35 kDa蛋白具有较强的抗原性。汉赛B.henselae与vero细胞共培养可提高IFA的反应性。这种反应性的增加在35 kDa条带中是显著的，然而，一些血清不与该条带反应。因此，我们不能找到唯一的抗原蛋白用于血清学诊断。因此，应考虑利用65、43和35 kDa的复合蛋白作为CSD的实验室诊断新方法。4.汉赛布氏杆菌能刺激淋巴管内皮细胞和血管内皮细胞的生长。汉赛B.henselae诱导外周中性粒细胞表面的CCR 7。该细菌还刺激淋巴管内皮细胞产生CCL 21。然后，我们研究了CCL 21在CSD患者中的诊断重要性。结果表明，CSD患者血清CCL 21水平与其他淋巴结疾病患者无显著性差异。5.建立了一种新的汉赛B.henselae的培养方法，并利用该方法从流浪猫中分离到16株汉赛B.

1.Analysis by SDS-PAGE of whole bacterial cells of Bartonella henselae revealed more than 35 protein bands. The molecular weight of major proteins were 90, 62, 45 (43), 40, 35, 31, and 25kDa. Molecular weight of the major outer membrane proteins were estimated as 62, 43 and 31kDa.2.Thirty-two of 108 patients who had swelling of lymph nodes and CSD was suspected showed more than 256x IgG titer against B.henselae by indirect immunofluorescence assay (IFA). These antibody positive sera were used for the following study.3.Western blotting analysis of whole bacterial cells of B henselae using patient sera revealed 8 bands (90, 65, 43, 40, 35, 33, 30, and 28kDa). Distribution pattern of positive bands were diverse in case to case, however, 65, 43, and 35kDa proteins showed strong antigenicity. The reactivity of IFA increased by co-cultivation of B.henselae with vero cells. This increase of reactivity were striking in 35kDa band, however, some sera did not reacted with this band. Consequently we could not find sole antigen protein useful for sero-diagnosis. Compound proteins, including 65, 43, and 35kDa protein, should be considered to develop new method for the laboratory diagnosis of CSD.4.B.henselae stimulated the growth of lymphatic endothelial cells as well as vascular endothelial cells. B.henselae induced CCR7 on the surface of peripheral neutrophils. The bacteria also stimulated the production of CCL21 from lymphatic endothelial cells. Then we examined the diagnostic importance of CCL21 in patients with CSD. The result showed no significant difference of serum CCL21 level between CSD and other patients with various lymph node diseases.5.We developed a new method to cultivate B.henselae, and isolated 16 strains of B.henselae from stray cats by using the method.