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Characterization of Envelopment Process of viral Nucleocapsids in Herpes Simplex Virus-Infected Cells

单纯疱疹病毒感染细胞中病毒核衣壳包封过程的表征

基本信息

批准号：
01570255
负责人：
KOYAMA Hajime
金额：
$ 1.28万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570255/
关键词：
Maturation Herpes simplex virus Brefeldin A The Golgi complex Formation of virion HSV-1 Envelopment Ammonium chloride 単純ヘルペスウィルス細胞内酸性小胞クロロキンモネンシン HSV-1

项目摘要

We have quantitatively studied an intracellular multiplication of herpes simplex virus type 1 (HSV-1) in Vero cells. Characterization of the inhibition of the multiplication of HSV-1 by ammonium chloride revealed that the formation of the virus particles (i, e., the envelopment of viral nucleocapsids) takes place in the intracellular acidic compartments and that the acidity in the compartments is essential for the envelopment. To examine the effect of the agent on the synthesis and transport of spike proteins of virus, the appearance of ability to adsorb the sensitized erythrocytes on the HSV-1-infected cell surface was characterized. The kinetics of appearance indicated that both synthesis and transport of spike proteins are not affected by the agent.Since the most acidic and the most agent-sensitive compartment among intracellular organelles is known to be the Golgi complex, we examined the role of the Golgi complex in the envelopment as well as in the formation of infectious progeny virus in the HSV-1-infected cells. Since Orefeldin A (BFA) is recently reported to cause disassembly of the Golgi complex, we characterized the effect of BFA on the HSV-1 multiplication, showing that (1) there are two different (i. e., agent-sensitive and -insensitive) pathways for the formation of virus particles in the HSV-1-infected cells and that (2) the infectious progeny virus arises exclusively from the agent-sensitive pathway.These results indicated the crucial role of the Golgi complex in the envelopment of HSV-1 that lead to the formation of the infectious progeny virus.

我们在Vero细胞中定量研究了1型单纯疱疹病毒（HSV-1）的细胞内增殖。氯化铵抑制HSV-1增殖的特性表明，病毒颗粒的形成（即病毒核衣壳的包膜）发生在细胞内的酸性区室中，而区室中的酸度对包膜的形成至关重要。为了研究该制剂对病毒刺突蛋白合成和转运的影响，对致敏红细胞在hsv -1感染细胞表面的吸附能力进行了表征。外观动力学表明，刺突蛋白的合成和运输不受药剂的影响。由于已知细胞内细胞器中酸性最强、对药物最敏感的是高尔基复合体，因此我们研究了高尔基复合体在hsv -1感染细胞的包膜以及感染性子代病毒形成中的作用。由于最近有报道称Orefeldin A （BFA）可导致高尔基复合体的分解，我们对BFA对HSV-1增殖的影响进行了表征，结果表明：(1)HSV-1感染细胞中病毒颗粒的形成有两种不同的途径（即药物敏感途径和药物不敏感途径）；(2)感染性子代病毒完全由药物敏感途径产生。这些结果表明高尔基复合体在HSV-1包膜中起关键作用，导致传染性子代病毒的形成。