A Seroepidemiological Study on The Risk of Infection with Hhrpes Simples Virus in Medical Staffs

医务人员单纯疱疹病毒感染风险的血清流行病学研究

• 批准号: 01570321
• 负责人: TOBA Masanori
• 金额: $ 0.45万
• 依托单位: Yokohama City University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570321/
• 关键词: Herpes Simplex Virus,; Risk in Medical Staffs,; Seroepidemiology,; Neutralizing Antibody,; ELISA; Seroprevalence; Seroconversion; 血清疫学研究; 抗体保有率; 医療従事者; 感染リスク

To elucidate the possibility that medical staffs in hospitals may be one of risk groups concerning infection with herpes simplex virus (HSV), seroepidemiological research was carried out using microneutralization test against HSV, and immunoglobulin quantitation by enzyme-linked immunosorbent assay (ELISA).The results obtained are summarized as follows :1. Positive rate of HSV antibodies in medical staffs examined was lower than that of general population in total, however it exceeded only in an age group of 20-24 years. The distribution of antibody titer in every age groups was similar, except that in the same group where titers of positive sera were accumulated at a higher range.2. Positive rate of nurse groups, especially in 20-24 years of age, were higher than that of general population, although that of physicians was significantly lower in each age group.3. Prevalence rate of nurses from 2 different hospitals were similarly higher than of normal population in early twenties, but … More a difference was observed between 2 groups.4. Young nurses working in departments for outpatients, or in surgical departments had higher prevalence rate than those working in wards for inpatients, or in departments of internal medicine, respectively.5. Prospective observation for 2 years revealed that seroconversion occurred mainly in nurses of 20-29 years of age, and overall seroconversion rate was 1.00% per person-years in total nurses. Meanwhile, significant rise of antibody titer was observed in 2.25% per person-years, and those who showed increment distributed evenly among age groups of 20 to 59 years.6. A method to assay immunoglobulin G specific to HSV by means of ELISA was established, although those for IgM, and IgA were in progress because of considerable levels of non-specific reaction. Titration of samples, therefore, is not accomplished.These results suggest that medical staffs in hospitals, especially young nurses, consist a risk group of HSV infection, and place or mode of works may affect the chance of infection. Less

为阐明医院医务人员可能是单纯疱疹病毒（HSV）感染的高危人群之一，采用微量中和试验和酶联免疫吸附试验（ELISA）免疫球蛋白定量方法对医院医务人员进行了血清流行病学研究。医务人员HSV抗体阳性率总体上低于一般人群，但仅在20-24岁年龄组高于一般人群。各年龄组的抗体滴度分布相似，但同一年龄组的抗体滴度累积范围较大.各年龄组护士组阳性率均高于普通人群，尤其是20-24岁年龄组，而医师组各年龄组阳性率均明显低于普通人群. 20岁出头2所医院护士患病率均高于正常人群， ...更多信息 两组之间观察到差异。门诊、外科年轻护士患病率高于住院、内科年轻护士.通过2年的前瞻性观察，发现血清阳转主要发生在20-29岁年龄段，总的血清阳转率为1.00%/人年。同时，抗体滴度以2.25%/人·年的速度显著上升，且20 ~ 59岁年龄段抗体滴度上升的人群分布均匀.建立了一种通过ELISA测定HSV特异性免疫球蛋白G的方法，尽管IgM和伊加的方法由于相当程度的非特异性反应而正在进行中。提示医院医务人员，尤其是年轻护士是HSV感染的高危人群，工作场所和工作方式可能影响HSV感染的机会。少