Investigation of gastric mucosal defense mechanisms using call culture

使用呼叫培养研究胃粘膜防御机制

• 批准号: 01570380
• 负责人: TERANO Akira
• 金额: $ 1.41万
• 依托单位: Faculty of Medicine, University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570380/
• 关键词: Mucosal defense; Stomaca; Cell culture; prostaglandin; free rodical; Cytoprotection; 粘腫防御; 細胞培養; プロスタグランディ-; 胃粘膜; viability; プロスタグラデイン; MTTアッセイ法; 培養細胞; 胃粘膜細胞保護作用; 情報伝達機構; グルタチオン

The treatment of peptic ulcer disease has been changed by the recent use of H2 blocker and proton pump inhibitor. The recurrence rate of peptic ulcer. however, has been still high and of important problem from the medical as well as social view point. For the elucidation of this issue, the mechanisms of gastric mucosal protection would be crucial. With respect to the protective mechanisms of gastroduodenal mucosa, the concept of prostaglandin cytoprotection or the role of free radicals would be of great importance. The most important point of this issue so far, is whether this protection depends on microcirculation of gastric mucosa and whether or not prostaglandins or other cytoprotective agents truly protect gastric mucosal cells per se. In this investigation, we have clarified that gastric mucosal cells were truly protected in invitro model, using gastric monolayer culture system developed by us previously. Furthermore, the mechanisms of endogenous prostaglandin production have been revealed by employing this system from the standpoint of molecular biology.

近年来，H2阻滞剂和质子泵抑制剂的应用改变了消化性溃疡的治疗。消化性溃疡的复发率。然而，从医学和社会的角度来看，仍然存在着严重的问题。为了阐明这一问题，胃粘膜保护的机制将是至关重要的。关于胃十二指肠黏膜的保护机制，前列腺素的细胞保护概念或自由基的作用将是非常重要的。到目前为止，这个问题最重要的一点是，这种保护是否依赖于胃粘膜的微循环，前列腺素或其他细胞保护剂是否真正保护胃粘膜细胞本身。在本研究中，我们利用我们之前开发的胃单层培养系统，明确了胃粘膜细胞在体外模型中确实受到保护。此外，利用该系统从分子生物学的角度揭示了内源性前列腺素产生的机制。

项目成果

H. Mutoh, S. Ota, H. Hiraishi, K. J. lvey, A. Terano, T. Sugimoto: "Reduced Glutathione Protects Cultured Gastric Mucosal Cells from Suckling Rats against Acid." American J. Physiology. 261. G65-G70 (1991)

H. Mutoh、S. Ota、H. Hiraishi、K. J. lvey、A. Terano、T. Sugimoto：“还原型谷胱甘肽可保护培养的胃粘膜细胞免受乳鼠胃酸的侵害。”

H.Mutoh,S.Ota,A.Terano.: "Protective pole of glutathion Against ethanel induced damage in aultured rat macosap cells." Am.T.phpiology.258. 603-609 (1990)

H.Mutoh，S.Ota，A.Terano.：“谷胱甘肽的保护极对抗乙烷诱导的大鼠粘液细胞损伤。”

S. Ota, A Terano, H. Mutoh, H. Hiraish, T. Sugimoto: "A Monolayer Cell Culture of Adult Rabbit Gastric Mucosal Cells." Gastroenterologia Japonica. 25. 1-7 (1990)

S. Ota、A Terano、H. Mutoh、H. Hiraish、T. Sugimoto：“成年兔胃粘膜细胞的单层细胞培养。”

S. Ota, H. Tsukahara, A. Terano, H. Hiraishi, H. Mutoh, Y. Hata, T. Sugimoto: "Protective Effect of Tauroursodeoxycholate against Chenodeoxycholate-induced Damage to Cultured Rabbit Gastric Cells." Digestive Diseases and Sciences. 36. 409-416 (1991)

S. Ota、H. Tsukahara、A. Terano、H. Hiraishi、H. Mutoh、Y. Hata、T. Sugimoto：“牛磺熊去氧胆酸对鹅去氧胆酸诱导的培养兔胃细胞损伤的保护作用。”

H.Hiraishi,A.Terano,S.Ota,H.Mutoh,M.Razandi,T.Sugimoto,K.J.lvey:"Role for Iron in Reactive Oxygen Speciesーmediated Cytotoxicity to Cultured Rat Gastric Mucosal Cells." American Journal of Physiology. 260. G556-G563 (1991)

H. Hiraishi、A. Terano、S. Ota、H. Mutoh、M. Razandi、T. Sugimoto、K. J. lvey：“铁在活性氧介导的培养大鼠胃粘膜细胞细胞毒性中的作用美国生理学杂志”。 .260.G556-G563（1991）