Role of trefoil peptide in gastric diseases

三叶肽在结肠息肉、结肠癌、炎症性肠病等胃肠道疾病中发挥重要作用。这些肽包括pS2基因、rITF和其它肽。TPs主要分布于胃、小肠和结肠等消化道粘膜。本课题旨在研究这些多肽在胃粘膜损伤和结肠损伤愈合过程中的作用。据报道，几种生长因子如EGF、TGF α、FGF和HGF可促进胃肠道粘膜损伤如消化性溃疡或克罗恩病、UC的愈合。我们测试了这些肽对大鼠胃中NSAID诱导的胃溃疡愈合的影响。首先，研究了rITF在大鼠胃和肠中的定位。rITF定位于大鼠胃、肠和结肠粘膜。此外，该肽在愈合阶段的胃溃疡边缘强烈表达。在本研究中，使用抗rITF抗体进行免疫染色。此外，我们还采用单层培养的胃粘膜细胞，观察TP对胃粘膜上皮细胞增殖和迁移的影响。结果表明，茶多酚对培养的胃粘膜细胞的增殖和迁移有明显的促进作用。我们计划对这些肽进行分子生物学研究，以阐明修复过程的作用机制。

Trefoil peptides(TP) are reported to play very important roles in various gastrointestinal diseases like colon polyp, colon cancer, inflammatory bowel diseases. This peptides include pS2 gene, rITF and other peptide.s. TPs were located in the mucosa of GI tracts including stomach, small intestine as well as colon. In this project, we attempted to investigate the role of these peptides in the healing process of gastric mucosal damage and colonic damage. Several growth factors like EGF, TGFa, FGF and HGF are reported to promote the healing of GI tract mucosal injuries like peptic ulcers or Crohn's disease, UC.We tested the effects of these peptides on the healing of gastric ulcer induced by NSAIDs in rat stomach. Firstly, the localization of rITF in rat stomach or intestine was investigated. rITF was located in the mucosa of rat stomach as well as intestine or colon. Further, this peptide was strongly expressed at the edge of the gastric ulcer on the healing stage. For this study, the immunostaining procedures were employed by using anti-rITF antibody. Moreover, we investigated the effects of TP on the proliferation and migration of epithelial cells by using the monolayer culture of gastric mucosal cells. As a result, TP strongly promoted the proliferartion and migration of gastric mucosal cells in culture. We are planning to study the molecular biological study of these peptides to elucidate the mechanisms of the action of repair process.