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Role of trefoil peptide in gastric diseases

三叶肽在胃病中的作用

基本信息

批准号：
09470142
负责人：
TERANO Akira
金额：
$ 8.38万
依托单位：
DOKKYO UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470142/
关键词：
Trefoil peptide pS2 gene rITF stomach colon peptic ulcer growth migration repair trefoil peptide 胃粘膜 mRNA 損傷修復

项目摘要

Trefoil peptides(TP) are reported to play very important roles in various gastrointestinal diseases like colon polyp, colon cancer, inflammatory bowel diseases. This peptides include pS2 gene, rITF and other peptide.s. TPs were located in the mucosa of GI tracts including stomach, small intestine as well as colon. In this project, we attempted to investigate the role of these peptides in the healing process of gastric mucosal damage and colonic damage. Several growth factors like EGF, TGFa, FGF and HGF are reported to promote the healing of GI tract mucosal injuries like peptic ulcers or Crohn's disease, UC.We tested the effects of these peptides on the healing of gastric ulcer induced by NSAIDs in rat stomach. Firstly, the localization of rITF in rat stomach or intestine was investigated. rITF was located in the mucosa of rat stomach as well as intestine or colon. Further, this peptide was strongly expressed at the edge of the gastric ulcer on the healing stage. For this study, the immunostaining procedures were employed by using anti-rITF antibody. Moreover, we investigated the effects of TP on the proliferation and migration of epithelial cells by using the monolayer culture of gastric mucosal cells. As a result, TP strongly promoted the proliferartion and migration of gastric mucosal cells in culture. We are planning to study the molecular biological study of these peptides to elucidate the mechanisms of the action of repair process.

据报道，三叶肽在结肠息肉、结肠癌、炎症性肠病等多种胃肠道疾病中发挥着重要作用。这些多肽包括PS2基因、RITF等多肽。TPS分布于胃、小肠、结肠等胃肠道粘膜。在本项目中，我们试图研究这些多肽在胃粘膜损伤和结肠损伤修复过程中的作用。几种生长因子如EGF、TGFa、FGF和HGF被报道促进消化性溃疡或克罗恩病等胃肠道黏膜损伤的愈合。我们测试了这些多肽对非甾体抗炎药所致大鼠胃溃疡愈合的影响。首先，研究了RITF在大鼠胃和肠中的定位。RITF定位于大鼠胃、肠、结肠粘膜。此外，该肽在胃溃疡愈合阶段的边缘有强烈的表达。在本研究中，采用了抗RITF抗体的免疫染色程序。此外，我们还采用胃粘膜细胞单层培养的方法，研究了茶多酚对胃粘膜上皮细胞增殖和迁移的影响。结果表明，茶多酚能显著促进体外培养的胃粘膜细胞的增殖和迁移。我们计划对这些多肽进行分子生物学研究，以阐明修复过程的作用机制。