Studies on glucose transporter and glucose transport system in human polymorphonuclear leukocyte

人多形核白细胞葡萄糖转运蛋白及葡萄糖转运系统的研究

• 批准号: 01570652
• 负责人: OKUNO Yasuhisa
• 金额: $ 1.22万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570652/
• 关键词: Glucose transport; Glucose transporter; Polymorphonuclear leukocytes; Diabetes mellitus; Glucocorticoids; 1, 5-anhydro-D-glucitol; 1.5ーアンヒドログリシト-ル; ステロイド; クッシング症候群

1. Clinical application of measurement of glucose transport in human polymorphonuclear leukocytes (PMNLs).Transport of 3-0-methyl-D-glucose (30MG) was measured at 37 ﾟC, pH 7. . 4 in PMNLs., The coefficient of variation of values, assayed on different days was about 7%. The transport rate did not differ between the two sexes or between subjects of different ages.The mean transport rate was significantly elevated in non-insulin-dependent diabetic patients compared with healthy controls (13.3 vs 10.4 fl/cell-sec, P<0.01). In the diabetics, transport rates were positively correlated with HbAlc levels but had no relations with ambient plasma glucose concentrations.The mean transport rate was prominently reduced in 5 patients with Cushing's syndrome compared with healthy controls. A significant negative correlation was found between transport rates and prednisolone doses given per day. Dexamethasone directly inhibited glucose transport rate in those cells time- and dose- dependent manners.2. Transport of 1, 5-anhydro-D-glucitol (AG) into PMNL.The uptake rate of AG into PMNL was much slower than that of 30MG. AG influx into PMNL is mediated not only by the glucose transport system but also via another facilitated transport system.3. Identification of glucose transporter isoform of PMNL.Norther blotting showed the presence of GLUT 1 mRNA. We are now studying whether other types of GLUTs also exist in those cells

1. 葡萄糖在人多形核白细胞（PMNLs）中转运测定的临床应用。3-0-甲基- d -葡萄糖（30MG）在37°C， pH = 7的条件下转运。PMNLs为4。不同日测定值的变异系数约为7%。转运率在两性和不同年龄的被试之间没有差异。与健康对照组相比，非胰岛素依赖型糖尿病患者的平均转运率显著升高（13.3 vs 10.4 fl/ cells -sec， P<0.01）。在糖尿病患者中，转运率与HbAlc水平呈正相关，但与周围血浆葡萄糖浓度无关。与健康对照组相比，5例库欣综合征患者的平均转运率显著降低。转运率与每天给药的强的松龙剂量之间存在显著的负相关。地塞米松直接抑制这些细胞的葡萄糖转运率，其方式具有时间和剂量依赖性。1,5 -无水- d -葡萄糖醇（AG）在PMNL中的转运。AG在PMNL中的吸收速率明显低于30MG。AG内流进入PMNL不仅由葡萄糖运输系统介导，还通过另一个便利的运输系统介导。PMNL葡萄糖转运蛋白异构体的鉴定。northern印迹显示GLUT - 1 mRNA的存在。我们现在正在研究其他类型的glut是否也存在于这些细胞中

项目成果

Yasuhisa Okuno: "Clinical application of measuremnt of glucose transport in human polymorphonuclear leukicytis" Diabetes Research and Clinical Practice. 7. S5-S9 (1989)

Yasuhisa Okuno：“人类多形核白细胞中葡萄糖转运测量的临床应用”糖尿病研究和临床实践。

Y.Okuno: "Inceased insulin-insensitive glucose transport in polymorphomuclear leuhocytes from non-prsalin-dependent diabitic patiens" Hormone and Metabolic Research. 23. 387-391 (1991)

Y.Okuno：“来自非普沙林依赖性糖尿病患者的多形核白细胞中胰岛素不敏感的葡萄糖转运增加”激素和代谢研究。

Y.Okuno: "Transport of 1,5-anhydro-D-glucitol into human polymorphosmuclear leukocytes" Journal of Biochemistry. 111. 99-102 (1992)

Y.Okuno：“将 1,5-脱水-D-葡萄糖醇转运到人多形核白细胞中”《生物化学杂志》。

Y. Okuno et al.: "Clinical application of measurement of glucose transport in human polymorphonuclear leukocytes" Diabetes Research and Clinical Practice. 7. S5-S9 (1989)

Y. Okuno 等人：“人类多形核白细胞中葡萄糖转运测量的临床应用”糖尿病研究和临床实践。

Y.Okuno: "Clinicel application of measmement glucose transport in human polyproyshonucleer leuhocytes" Diabites Research and Clinicel Practice. 7. S5-S9 (1989)

Y.Okuno：“测量葡萄糖转运在人多原核白细胞中的临床应用”糖尿病研究和临床实践。