Studies on glucose transporter and glucose transport system in human polymorphonuclear leukocyte

1.人多形核白细胞（PMNLs）葡萄糖转运测定的临床应用在37 ℃、pH 7条件下测定3-0-甲基-D-葡萄糖（30 MG）的转运。. PMNL中4例，在不同日期测定的值的变异系数约为7%。非胰岛素依赖型糖尿病患者的平均转运率显著高于健康对照组（13.3 vs 10.4 fl/cell-sec，P<0.01）。糖尿病患者的转运率与HbAlc水平呈正相关，与周围血糖浓度无关，5例库欣综合征患者的平均转运率明显低于健康对照组。一个显着的负相关性之间的运输率和每天给予泼尼松龙剂量。地塞米松直接抑制细胞葡萄糖转运率，并呈时间和剂量依赖性. PMNL对1，5-脱水-D-葡萄糖醇（AG）的摄取速率明显慢于30 MG。AG流入PMNL不仅通过葡萄糖转运系统介导，还通过另一易化转运系统介导.结果表明，PMNL细胞中存在葡萄糖转运蛋白1（GLUT 1）mRNA.我们现在正在研究这些细胞中是否也存在其他类型的GLUT

1. Clinical application of measurement of glucose transport in human polymorphonuclear leukocytes (PMNLs).Transport of 3-0-methyl-D-glucose (30MG) was measured at 37 ﾟC, pH 7. . 4 in PMNLs., The coefficient of variation of values, assayed on different days was about 7%. The transport rate did not differ between the two sexes or between subjects of different ages.The mean transport rate was significantly elevated in non-insulin-dependent diabetic patients compared with healthy controls (13.3 vs 10.4 fl/cell-sec, P<0.01). In the diabetics, transport rates were positively correlated with HbAlc levels but had no relations with ambient plasma glucose concentrations.The mean transport rate was prominently reduced in 5 patients with Cushing's syndrome compared with healthy controls. A significant negative correlation was found between transport rates and prednisolone doses given per day. Dexamethasone directly inhibited glucose transport rate in those cells time- and dose- dependent manners.2. Transport of 1, 5-anhydro-D-glucitol (AG) into PMNL.The uptake rate of AG into PMNL was much slower than that of 30MG. AG influx into PMNL is mediated not only by the glucose transport system but also via another facilitated transport system.3. Identification of glucose transporter isoform of PMNL.Norther blotting showed the presence of GLUT 1 mRNA. We are now studying whether other types of GLUTs also exist in those cells