Locomotor Changes of Cat Forelimb and CNS-Metabolic Changes using Cytochrome oxidase Histochemistry and 2-DG After Destrction of Medullary Pyramis or Cervical Dorsal Roots

运动恢复尚不完全清楚，因此我们用细胞色素氧化酶组织化学(Wong-Riley，1 976)和~(14)C-脱氧葡萄糖放射自显影(Sokoloff et a]，1077)研究了延髓锥体切除猫的运动障碍和代谢变化。运动功能检查包括进食运动(Gorska等人，1980)和体格检查。对术后1周的4只猫和术后7周的2只猫进行细胞色素氧化酶组织化学染色。所有的猫都有正常的休息姿势和行走能力，但不能从圆柱体中取出食物。所有猫均表现为对侧屈肌促进和伸肌抑制。组织化学显示所有猫均无偏侧性。4只猫手术后1wk的C脱氧葡萄糖放射自显影显示，同侧丘脑VPL核局部脑葡萄糖代谢率降低。然后用WGA-HRP法研究了经颈前根与一肌有关的中枢神经系统。切断C4~C8背根，将WGA-HRP植入周围神经。手术后两天，我工作，猫被作为组织学的牺牲品。双侧前角细胞、后角细胞、背柱核、下橄榄核、小脑核、梯形体核、红核、黑质、丘脑核团和内囊均有反应。如此多的地点显示了一种与运动恢复有关的可能性。

Motor recovery is not fully understood, so we studied motor disturbance and metabolic changes in medullary pyramidotomized cats using cytochrome oxidase histochemistry(Wong-Riley, 1 976)and 14C-deoxiglucose autoradiography(Sokoloff et a]., 1077). Motor function was examined as food-taking movement(Gorska et al., 1 980)and physical examination. Cytochrome oxidase histochemistry was done to 4 cats I wk after the surgery and to 2 cats 7 wks after the surgery. All cats had normal resting posture and ambulation, but could not retrieve food from within a cylinder. The all cats showed contralateral flexor facilitation and extensor inhibition. The histochemistry showed no laterality on the all cats. 14Cdeoxiglucose autoradiography I wk after the surgery was done for 4 cats, showed that the local cerebral metabolic rate for glucose decreased in the ipsilateral thalamic VPL nucleus. Then we studied CNS concerning to one muscle via cervical ventral roots using WGA-HRP. The dorsal roots C4 to C8 were cut and WGA-HRP was implanted to the peripheral nerve. Two days and I wk after the surgery, cats were sacrificed to histology. Bilateral anterior horn cells, posterior horn cells, dorsal column nuclei, inferior olives, cerebellar nuclei, trapezoid bodies, red nuclei, substantia nigras, thalamic nuclei, and internal capsules were reacted. These so many sites showed one possibility concerning to the motor recovery.