Effect of Centrifugal Force on Chondrocytes from Craniofacial Complex

为探讨生物力学对颅面复合体软骨细胞的影响，将鼻中隔和下颌骨软骨细胞作为致密块保存在离心管中。细胞在CO_2培养箱中用离心机离心。在0.3%血清条件下，27 g离心的鼻中隔软骨细胞引起[^3S<35>]硫酸盐掺入GAG和[^3H]胸苷掺入DNA的2倍增加。另一方面，下颌骨软骨细胞以27 g离心24小时导致[^<35>S]硫酸盐掺入GAG增加2倍。然而，下颌骨软骨细胞在27 g离心24 h后，[^3H]胸苷掺入DNA的作用降低了50%。鼻中隔软骨细胞在沉淀培养物中表达低的ALP13活性。然而，在10%血清下以27 g离心鼻中隔软骨细胞23天，导致ALP4活性增加10倍。下颌骨软骨细胞在颗粒状培养物中表达高的ALP13活性。下颌骨软骨细胞在10%血清下以27 g离心7天，导致ALP12活性增加两倍。这些结果表明，生物力学力对鼻中隔和下颌骨软骨细胞的生长有不同的影响，并刺激软骨细胞在培养中的分化和钙化。

To investigate the effect of biomechanical forces on chondrocytes form craniofacial complex, nasal septal and mandibular chondrocytes were maintained in centrifuge tubes as Packed mass. Cells were centrifuged with a centrifuge in a CO_2 incubator. The centrifugation of nasal septal chondrocytes at 27 g caused a two-fold increase in both [^<35>S]sulfate incorporation into GAG and [^3H]thymidine incorporation into DNA under 0.3% serum. On the other hand, the centrifugation of mandibular chondrocytes at 27 g for 24 hours caused a 2-fold increase in [^<35>S]sulfate incorporation into GAG. However, the centrifugation of mandibular chondrocytes at 27 g for 24 hours caused 50% decrease effect on [^3H]thymidine incorporation into DNA. Nasal septal chondrocytes express low ALPase activity in pelleted cultures. However, the centrifugation of nasal septal chondrocytes at 27 g for 23 days under 10% serum caused a ten-fold increase in ALPase activity. Mandibular chondrocytes express high ALPase activity in pelleted cultures. The centrifugation of mandibular chondrocytes at 27 g for 7 days under 10% serum caused a two-fold increase in ALPase activity. These results suggested that biomechanical forces have differential effects on growth in nasal septal and mandibular chondrocytes, and stimulate the differentiation and calcification of chondrocytes in culture.