"Study on physical properties of inositol phospholipid in cellular signal transduction"

点击翻译按钮获取中文摘要

Phosphatidic acids play an important role in cellular function ; for example, proliferation of a cell, signal transduction on plasma membranes and mobilization of calcium ions. In the present study, interactions of phosphatidic acid with poly (L-lysine) and calcium ions were investigated by differential scanning calorimetry and X-ray diffraction technique. Results on phosphatidic acid/poly (L-lysine) system indicate that poly (L-lysine) interacts with phosphatidic acid electrostatically without affecting the chain-packing of the lipid bilayer membranes and that the interaction does not obey the one-to-one sto ichiometry. Further analysis of X-ray diffraction data gives a probable model of phosphatidic acid/poly (L-lysine) membranes. Calorimetric study on phosphatidic acid/ (phosphatidylcholine and/or phosphatidylethanolamine) /Ca2+ system elucidates a fine structure of membranes composed of the mixed phospholipids. In these membranes, phosphatidic acid is not completely segregated by Ca2+ but studded in the membranes. The present study is helpful to proceed a research of relationship between membrane structure and function.