Development and Application of Insect Cell Culture Techniques

昆虫细胞培养技术的开发与应用

基本信息

  • 批准号:
    01860008
  • 负责人:
  • 金额:
    $ 7.49万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B).
  • 财政年份:
    1989
  • 资助国家:
    日本
  • 起止时间:
    1989 至 1990
  • 项目状态:
    已结题

项目摘要

Infection and development of microsporidians (Nosema bombycis NIS 001, N. sp. NIS M11, N. sp. F-30, N. mesnili AH, Thelohania sp. K-25) pathogenic to lepidopterous insects were studied in a cell line of Antheraea eucalypti. Microsporidian spores primed with 0.1 N potassium hydroxide solution were mixed with the A. eucalypti cell cultures and infected cultures were maintained for more than 18 months.Low cost insect cell culture media (MM) and its application to spinner culture of insect cells were investigated. Growth of two lepidopteran and one dipteran cell lines was compared under still and spinner culture conditions. Apparently, the insect cells cultured in plastic culture flasks showed higher growth rates when smaller culture vessels were employed.Also, a general purpose culture medium (MGM-450) for insect (Lepidopera, Diptera, Hemiptera and Coleoptera) cells was newly formulated.Cloning of insect cells using Bombyx mori cell line (SES-BoMo-15AII) was studied and establishment of the B. mori cell line resulted from a single cell was unsuccessful. It was suggested that a colonial coloning was practical method for cloning of insect cells at the present time.Spodoptera furgiperda Sf21AEII cells were highly sensitive to heat labile cytopathic factor (s) produced by several serovars of Bacillus thuringiensis. However, in vitro assay of the heat stable exotoxin (fly toxin) of B. thuringiensis using the similar system showed negative results.Addition of insect hemolymph factor (s) to Bombyx mori BM-N cells infected with a nuclear polyhedrosis virus, markedly enhanced the production of viral structural and inclusion proteins in vitro.Specific and common cell surface antigens of cultured lepidopteran cells were demonstrated and serological identification of insect cell lines was applicable for several insect cell lines.
家蚕微孢子虫Nosema bombycis NIS 001、N. nisM 11,新脉轮藻N. sp. F-30,N. mesnili AH,Thelohania sp. K-25)对鳞翅目昆虫的致病性。将用0.1N氢氧化钾溶液引发的微孢子虫孢子与A.本实验研究了低成本昆虫细胞培养基(MM)及其在昆虫细胞转瓶培养中的应用。在静止和旋转培养条件下比较了两种鳞翅目和一种双翅目细胞系的生长。本研究还配制了一种昆虫细胞通用培养基(MGM-450),并利用家蚕细胞系(SES-BoMo-15 AII)进行了昆虫细胞克隆的研究,建立了昆虫细胞克隆的B.从单个细胞产生的Mori细胞系是不成功的。结果表明,集落克隆法是目前昆虫细胞克隆的一种实用方法,且对苏云金芽孢杆菌(Bacillus thuringiensis)的几个血清型产生的不耐热细胞病变因子高度敏感。然而,在体外测定B的热稳定外毒素(蝇毒素)。在家蚕BM-N细胞中加入昆虫血淋巴因子,能显著提高病毒结构蛋白和包涵体蛋白的产量,证明了鳞翅目昆虫细胞表面的特异性和共同性抗原,并证明了昆虫细胞系的血清学鉴定适用于几种昆虫细胞系。

项目成果

期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Takeshi Kawarabata: "Infection and development of Thelohania sp. K-25 (Microsporida : Thelohaniidae) in a lepidopteran cell line." Applied Entomology and Zoology. 26. (1991)
Takeshi Kawarabata:“Thelohania sp. K-25(微孢子虫:Thelohaniidae)在鳞翅目细胞系中的感染和发育。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takeshi Kawarabata: "Infection and development of <Thelohania>___ー sp.Kー25 (Microsporida:Thelohaniidae) in lepidopteran cell lines." Applied Entomology and Zoology. 26. (1991)
Takeshi Kawarabata:“鳞翅目细胞系中 <Thelohania>___ sp.K-25(微孢子虫:Thelohaniidae)的感染和发育。”应用昆虫学和动物学 26。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
永田 昌男: "カイコ由來培養細胞表面抗原の酵素免疫測定法による解析" 日本蚕糸学雑誌. 60. (1991)
Masao Nagata:“酶免疫分析培养蚕细胞表面抗原”,日本血清学杂志 60。(1991)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Jyun Kobayashi, Hajime Inoue, and Sigeo Imanishi: "Establishment and viral susceptibility of a Bombyx mori cell line from ovarian tissues of Cambodge strain. (In Japanese)" Journal of Sericultural Science of Japan. 60. (1991)
Jyun Kobayashi、Hajime Inoue 和 Sigeo Imanishi:“来自 Cambodge 品系卵巢组织的家蚕细胞系的建立和病毒敏感性。(日语)”日本蚕业科学杂志。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Michihiro Kobayashi: "Protein synthesis in BMーN cells infected with <Bombyx>___ー <mori>___ー nuclear polyhedrosis virus." Journal of Invertebrate Pathology. 57. (1991)
Michihiro Kobayashi:“感染 <Bombyx>___- <mori>___- 核多角体病毒的 BM-N 细胞中的蛋白质合成。无脊椎动物病理学杂志 57。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

KAWARABATA Takeshi其他文献

KAWARABATA Takeshi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('KAWARABATA Takeshi', 18)}}的其他基金

Study on karyotype and genome size of entomopathogenic microsporidians
昆虫病原小孢子虫核型和基因组大小研究
  • 批准号:
    11460027
  • 财政年份:
    1999
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
BIOLOGICAL STUDIES ON ANCESTORAL INSECT SPECIES OF THE SILKWORM,BOMBYXMORI
家蚕昆虫祖先种的生物学研究
  • 批准号:
    07406004
  • 财政年份:
    1995
  • 资助金额:
    $ 7.49万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了