Study on karyotype and genome size of entomopathogenic microsporidians

昆虫病原小孢子虫核型和基因组大小研究

基本信息

批准号：
11460027
负责人：
KAWARABATA Takeshi
金额：
$ 7.74万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The identification of microsporidian parasites has been based on morphological characters observed with light and electron microscopy. Pulsed-field gel electrophoresis has recently provided an alternative for not only the investigation of chromosomes in small single-celled organisms but also the identification of microsporidians. On the other hand, microsporidians used in those studies were not cloned ones. Mixed infection of microsporidians has been known from the diseased silkworm larvae. In this study, the interaction of the parasites in insect hosts and insect cell cultures under mixed infection was investigated first and estimate of the karyotype of the cloned microsporidians was made by light microscopy. Then, we presented data from pulsed-field gel electrophoresis of cloned microsporidians and the intra- and inter-specific variations of entomophathogenic microsporidians was discussed.Both in the silkworm and in the Spodoptera frugiperda SF21AEII cell culture, Nosema bombycis NIS … More 001 dominated on the other microsporidians, Nosema sp. NIS M11 and Vairimorha sp. NIS M12, when it was involved in the mixed infections, However, the dominant did not eliminate the minor ones by competition and microsporidians could co-exist in the same host. It was, therefore, strongly suggested that cloning of microsporidians would be important when the characterization and identification of the parasites were discussed.Several clones of those microsporidians in insect cell cultures were established by a limiting dilution method and attempt to estimate the number of chromosomes for the vegetative cells of cloned microsporidians was made by an Axioplan 2 universal microscope. Thirteen chromosomes were visualizied for the microsporidian, Vairimorpha sp. NIS M12. In genomic DNA analysis of microsporidia, it was clarified that alkali EDTA solution treatment of spore was needed for genome extraction from Brazilian isolates. The banding patterns were different among the species of microsporidia in pulse-field gel electrophoresis, suggesting that this method useful tool for identification of microsporidia. Established protocol in this report could apply for all isolates used, and this restult demnonstrated the importance of cloning. Less

微孢子寄生虫的鉴定是基于使用光和电子显微镜观察到的形态特征。最近，脉冲凝胶电泳不仅为小型单细胞生物中的染色体进行了研究提供了替代方案，而且还为微孢子虫的鉴定提供了替代方案。另一方面，这些研究中使用的微孢子虫不是克隆的。从解剖的蚕幼虫中知道了微孢子虫的混合感染。在这项研究中，首先研究了昆虫宿主和昆虫细胞培养物中寄生虫在混合感染下的相互作用，并通过光学显微镜进行了克隆的微孢子虫的核型估计。然后，我们介绍了克隆微孢子虫的脉冲场凝胶电泳以及昆虫病原体微孢子虫的特异性变化的数据。 NIS M11和Vairimorha sp。然而，当NIS M12参与混合感染时，主导者并没有通过竞争来消除次要感染，而微孢子虫人可以共存在同一宿主中。因此，强烈建议在讨论寄生虫的表征和识别时，微孢子虫的克隆将很重要。这些微孢子虫在昆虫细胞培养物中的几个克隆是通过极限稀释方法建立的，并试图估计由小孢子型植物性植物性植物型偶像型轴体估算染色体的数量。为Microsporidian Vairimorpha sp。可视化了13个染色体。 NIS M12。在微孢子虫的基因组DNA分析中，可以澄清说，从巴西分离株中提取基因组需要对孢子的碱性溶液处理。在脉冲场凝胶电泳中，微孢子虫物种之间的带模式不同，这表明该方法可用于鉴定微孢子虫。本报告中建立的协议可以适用于所有使用的分离株，这恢复了克隆的重要性。较少的

项目成果

期刊论文数量（54）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

寺元理恵ら: "ブラジルの養蚕農家で発生した微胞子虫病と病原(3)未同定微胞子虫について"九州蚕糸. 30. 23 (1999)

Rie Teramoto等：“巴西养蚕农民中发生的微孢子虫疾病和病原体（3）关于不明小孢子虫”九州芹彦（Kyushu Serihiko）。

DOI：
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期刊：
影响因子：
0
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通讯作者：

寺元理恵,青木智佐,橋口美保子,河原畑勇,富丸英博: "ブラジルの養蚕農家で発生した微胞子虫病と病原(4)2種類の胞子を産生する微胞子虫について."第56回日本蚕糸学会九州支部研究発表会並びに学術講演会. 1. 21 (2000)

Rie Teramoto、Chisa Aoki、Mihoko Hashiguchi、Isamu Kawarabata、Hidehiro Tomimaru：“巴西养蚕农民中发生的微孢子虫疾病和发病机制（4）关于产生两种类型孢子的小孢子虫第 56 号日本丝绸学会九州分会研究报告和”学术讲座。 1. 21 (2000)