Control and epidemiology of animal agent diseases and their biology in Southeast Aisia Epidemiology phylogenic analysis related to pathogenicity in human, and control of diseases caused by animal agent in South east Asia.

东南亚动物病原体疾病及其生物学的控制和流行病学与人类致病性相关的流行病学系统发育分析，以及东南亚动物病原体引起的疾病的控制。

• 批准号: 02044142
• 负责人: TAKAI Kenji
• 金额: $ 1.28万
• 依托单位: Department of Medical Zoology, st Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02044142/
• 关键词: Vector control; lasar light; sterilization; chemical; attractant; wing beat sound; phylogeny; Opistorchis; 09Clonorchis; 10Paragonimus; 11Angiostrongylus; 12electromorph; 13mitochondria DNA; 14discriminate

-1 Medical EntomologyThe studies were performed in order to develop control method by analyzing the biological characters of the vectors of diseases in Southeast Asia from the point of their physiology and ecology, emphasizing physical and chemical stimulation without insecticide application in their control. Irradiation of lasar light on mosquitoes : After the irradiation was introduced to Culex pipiens " molestus", using 5mW/m of HeーNe lasar light for 10 second the mosquitoes irradiated were paired with non treated ones, kept and maintained for several generations. The efficacy of the sterilization was proven when the group of 1-2 days after immersion showed highest sterility effect and their sterility lasted to 4th generations, being 50 % of larval mortality of their offspring. Attractancy for bloodfeeding of Culex quinquefastiatus : The attractancy was tested on Culex quinquefastiatus. Animal and chemical stimulation experiments were performed. The insemination of mosquito induces … More the active biting behavior of female. The chemicals which attracted effectively in high number of mosquitoes were octanol, n-butyric acid, 1-lacrtic acid, 1-nonanol, (s)(+)-2-nonaol and 5-methyl-3-heptanol. A trap which emits and evaporate modified wing beat sound, carbondioxide and animal scent for mosquito vectors was designated and applied for patent due to excellent success of field application in tropical areas. Integrated uses of the trap and an insect growth regulator, pyriproxyfen have proven their utilily fily for control of vectors of Japanese encephlitis, filariasis, dengue and dengue hemorrhagic feyer and malaria, and monitoring of the various vectors in tropical field as well as in seasonal pervarence of Culex tritaeniorhynchus in Japan.-2 Studies on Helminthic DiseasesStudies on the relation between phylogeny and pathogenicity of the agent organisms :Incidental parasites Paragonimus in Thailand and Japan were compared among sympatric and allopatric species. The species with similar pathogenicity, P. miyazakii and P. heterotremus had closer genetic relationships than the other species of sympatic one.Liver fluke in Thai, Opistorchis vivelini and Clonorchis sinensis in Japan and Korea are being clinicopathologically and epidemiolo-parasitologically investigated. Though the studies are still on going, Phylogenic investigation suggested a number of morphological variations were proved in the northeast area of Thailand.Phylogenic studies on Angiostrongylus proven from the point of electromorph and mitochondrial DNA discriminate are still on going since last 3years. Intraspecific variations and distribution are in under investigation. Less

医学昆虫学从生理生态学的角度分析东南亚地区病媒生物的生物学特性，强调物理和化学刺激，而不是使用杀虫剂进行控制，以制定防治方法。激光照射蚊子：用5mW/m的He / Ne激光照射10秒后，照射后的蚊子与未照射的蚊子配对，保持数代。灭菌效果在浸泡1 ~ 2天时达到最高，灭菌持续至第4代，为其后代幼虫死亡率的50%。致倦库蚊吸血引诱：对致倦库蚊进行引诱试验。进行动物和化学刺激实验。蚊子的授精更能诱导雌蚊的主动叮咬行为。辛醇、正丁酸、1-乳酸、1-壬醇、(s)(+)-2-壬醇和5-甲基-3-庚醇对蚊虫的引诱效果较好。研制了一种散发和蒸发改性翅拍声、二氧化碳和动物气味的蚊媒诱捕器，在热带地区的现场应用取得了优异的效果，并申请了专利。该诱捕器与昆虫生长调节剂吡丙醚的综合使用已被证明在控制日本脑炎、丝虫病、登革热、登革出血热和疟疾病媒以及监测日本热带地区各种病媒和三带喙库蚊的季节性传播方面具有实用性。-2寄生虫病的研究病原生物系统发育与致病性关系的研究：比较了泰国和日本同域种和异域种的偶发寄生虫并殖吸虫。致病性相似的两种，宫崎假单胞菌和异tremus假单胞菌的亲缘关系比同感型假单胞菌的亲缘关系更密切。对泰国的肝吸虫、日本和韩国的华支睾吸虫进行了临床病理学和流行病学调查。虽然研究仍在进行中，但系统发育调查表明，在泰国东北部地区已经证实了一些形态变异。近3年来，从电形态和线粒体DNA区分的角度对管圆线虫进行了系统发育研究。种内变异和分布正在调查中。少

项目成果

I.Sawabe and T.Kanda: "Studies on the effective attractants of anopheline mosquitoes 1.Bloodーfeeding,mating and flight activity of female Anopheles balabacensis(Diptera:Culicidae)." Appl.Ent.Zool.25. 231-238 (1990)

I.Sawabe 和 T.Kanda：“按蚊有效引诱剂的研究 1.雌性按蚊（双翅目：蚊科）的吸血、交配和飞行活动。”231-238（ 1990)

S.Leemingsawat,T.Kanda et al.: "Responce and monitoring of the vectors of Japanese encephalitis by sound trap after application of two control methods." Jpn.J.Trop.Med.& Hyg.18. 75-76 (1990)

S.Leemingsawat、T.Kanda 等人：“应用两种控制方法后，通过声音陷阱对日本脑炎媒介进行响应和监测。”

Supatra T.: "Atrial for control of both senes of Cnlex tritaeniorhynuchus by sound trapping at the breeding size" Mosq.Bome.Dis.Bull.7. 89-94 (1990)

Supatra T.：“通过在繁殖规模上进行声音捕获来控制 Cnlex tritaeniorhynuchus 两个系的心房”Mosq.Bome.Dis.Bull.7。

Chalit Komalamisra: "Phylogenetic analysis based an isozyme analysis of three spocies of Parahominus,human incidental parasites in Thailand and Japan" St.Marianna Med.Journ.18. 189-198 (1990)

Chalit Komalamisra：“基于对泰国和日本人类偶然寄生虫三种副人菌同工酶分析的系统发育分析”St.Marianna Med.Journ.18。

T.Ikeshoji: "Induction of inherited sterility and arval mortality to mosquitoes by laser radiation." Jpn.J.Sanit.Zool.(in press). (1991)

T.Ikeshoji：“通过激光辐射诱导蚊子遗传性不育和虫害死亡率。”