Host-pathogen specificity and a control of the expression of active resistant genes

研究了豌豆病原菌Mycosphaerelia pinodes产生的激发子和抑制子对PAL和CHS基因在豌豆主动防御反应中的信号转导和调控作用.对质膜（PM）ATP酶的作用方式。抑制子不仅抑制豌豆的PM-ATPase，而且在体外试验中也抑制其他植物的PM-ATPase，但细胞学研究表明它只特异性地抑制豌豆的PM-ATPase。此外，PMATPase的非特异性抑制剂原钒酸盐在延迟PAL和CHS表达诱导方面的作用与真菌抑制剂非常相似.对PM磷脂酰肌醇（PI）代谢的影响。诱导子可促进PIP和PIP 2的磷酸化，而抑制子则明显抑制PIP和PIP 2的磷酸化. PAL基因的结构及其表达调控。PAL基因的两个成员（PSPALI）的结构和基因组织 关于我们 和PSPAL 2），并利用序列特异性探针检测各成员转录本在组织中的积累。在根和茎的下部观察到两种转录物的高水平积累，但在其他器官中很少，这表明PSPAL 1和PSPAL 2可能含有负责组织特异性表达的顺式元件，特别是在根中。此外，顺序删除的启动子片段的PSPALI连接到植物表达载体含有报告基因被引入豌豆原生质体通过电穿孔，诱导诱导和抑制原钒酸盐进行了检查。PSPALI.4的-340100之间存在负责这些规定的顺式元件。CHS基因的结构和组织。豌豆CHS基因（PSCHS 1、PSCHS 2）形成一个具有间隔区的串联重复簇，并且含有盒I的共有基序的相同的31 bp序列位于两个CHS基因的推定TATA盒的5 '上游。少

Effects of elicitor and suppressor produced by a pea pathogen, Mycosphaerelia pinodes, were examined on the signal transduction and the regulation of the genes encoding PAL and CHS in pea active defense responses.1. Mode of action on plasma membrane (PM) ATPase. Suppressor inhibits PM-ATPase not only from pea but also from other plant species tested in in vitro assay, but cytological studies showed that it specifically inhabits only pea PM-ATPase. Moreover, orthovanadate, a non-specific inhibitor for PMATPase, functions in delaying the induction of the expression of PAL and CHS in a manner very similar to a fungal suppressor.2. Effects on PM phosphoinisitides (PI) metabolisms. The phosphorylation of PIP and PIP2 by lipid kinase was stimulated by the treatment with elicitor, but it was distinctively suppressed in the concomitant presence of suppressor.3. Structure of PAL genes and the control of the gene expression. Structure and gene organization of the two members of PAL gene (PSPALI … More and PSPAL2) in a small multigene family were determined, and the accumulation of the transcripts of each member in the tissues was examined using sequence specific probe. High level of the accumulation of both transcripts'was observed in roots and the lower portion of the stems, but very little in other organs, which suggests that PSPALI and PSPAL2 may contain ciselement responsible for tissue specific expression particularly in roots. Furthermore, the sequentially deleted promoter fragment of PSPALI connected to a plant expression vector containing the reporter gene was introduced into pea protoplasts by electroporation, and induction by elicitor and suppression by orthovanadate was examined. Cis- elei-nent responsible for these regulations exists between -340100 of PSPALI.4. Structure and organization of CHS gene. Pea CHS genes (PSCHSI, PSCHS2) form a cluster in a tandem repeat with a spacer, and an identical 31 bp sequence containing, the consensus motif of box I locates 5'-upstream from the putative TATA box of both CHS genes. Less