Expression von Vascular Endothelial Growth Factor (VEGF) in retinalem Pigmentepithel bei altersabhängiger Makuladegeneration (Expression of angiogenic factors and their regulation in human retinal pigment epithelium of patients with age-related macular de

在老年性黄斑变性(AMD)中，脉络膜新生血管(CNV)反映了局部血管调节功能障碍。因此，血管调节有望成为AMD治疗干预的关键。正确的脉络膜毛细血管维护需要完整的视网膜色素上皮细胞(RPE)。在这一点上，无论是生理上活跃的局部血管调节机制或它们在AMD中的机会都不是很清楚。在DFG(Ha 2537/2-1)批准的当前项目中，我们从人CNV标本中建立了RPE细胞培养。我们开始用分子生物学的方法比较新生血管生成介质及其受体在CNV来源的培养和对照RPE培养中的表达。不同刺激物对RPE-脉络膜毛细血管信号传导的影响将分别通过RT-PCR、Northern印迹、Western印迹和免疫组织化学方法在转录和翻译水平上进行评估。

In age-related macular degeneration (AMD), choroidal neovascularization (CNV) reflects dysfunctional local angioregulation. Therefore, angioregulation is expected to hold keys to therapeutic interventions in AMD. Proper choriocapillaris maintenance demands intact retinal pigment epithelial cells (RPE). At this point, neither thephysiologically active local mechanisms of angioregulation nor their chances in AMD are sufficiently understood. In a current project granted by DFG (Ha 2537/2-1) we established RPE cell cultures from human CNV speciments. We started to compare the expression of angiogenic mediators and their receptors in CNV-derived cultures with control RPE cultures using tols of molecular biology. The influence of different stimuli with a putative role in RPE-choriocapillaris signalling will be assessed on the transcriptional and translational level by RT-PCR, northern blot, western blot, and immunohistochemistry, respectively.