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疎水性高分子膜内へのタンパク質の配向分散と膜の生体膜モデルとしての利用

蛋白质在疏水聚合物膜中的定向分散以及使用膜作为生物膜模型

基本信息

批准号：
02805110
负责人：
HORI Teruo
金额：
$ 1.02万
依托单位：
Fukui University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02805110/
关键词：
Biomimetic membrane protein composite membrane electric field method cast membrane electrone transfer function membrane potential selective permeability conjugated redox reaction ポリペプチド複合膜共役酸化還元機能膜電子伝達機能膜 αーヘリックス構造溶質透過

项目摘要

1. Gelatin(GEL)and poly(ethylene terephthalate)(PET)were dissolved in their common solvent, hexafluoro-2-propanol(HFIP)to make homogeneous solution. Using this solution, GEL/PET Composite membranes were prepared in the absence and in the presence of an electric field.2. The structure and some properties of the GEL/PET(2/8)composite membranes were investigated by means of microscopy, differential scanning calorimetry, measuring of the membrane potential. It was found that the GEL parts in the membrane elongate parpendicular to the membrane surface and therefore those parts are passed through the membrane from one surface to the opposite surface. It was also pointed out that the structure was quite similar to that of cell membranes.3. High permeation selectivity was found for the permeation of glucose and phenol through the GEL/PET membranes. In addition, the pH dependence of the permeability was quite different among the solute.4. Across the Cu^<2+>-doped composite membranes a conjugated redox reaction, thioglicolic acid / methylene blue, could be drived. From this it was shown that the membrane acts as a electrone transfer membrane.5. Same oriented inner structure was found in the membranes prepared from polypeptide and polystyrene.6. The permeation of some electrilytes through the GEL/PET and PBG-PGA/PS membranes were measured ; the order of the permeability could be explained either using Stkes radius or considering the aggregates from alpha-helix structure.7. In the case of driving of conjugated redox reaction across the Sericin/PET membrane, on which enzymes were immobilized, the reaction was accelerated, though the enzyme activity was very low. This could be explained by the fact that the electrone transfer on the membrane surface is very efficient.8. From the fact described above, it could be concluded that the membranes prepared by casting method in the presence of an electric field can be regarded as a good cell membrane model.

1.将明胶（GEL）和聚（对苯二甲酸乙二醇酯）（PET）溶解在它们的共同溶剂六氟-2-丙醇（HFIP）中以制备均匀溶液。使用该溶液，在不存在和存在电场的情况下制备了GEL/PET复合膜.用显微镜、差示扫描量热法、膜电位测定等方法研究了GEL/PET（2/8）复合膜的结构和性能。结果发现，膜中的凝胶部分伸长到膜表面，因此这些部分从一个表面穿过膜到达相对表面。并指出其结构与细胞膜的结构十分相似.结果表明，该膜对葡萄糖和苯酚具有较高的渗透选择性。此外，不同溶质对渗透性的pH依赖性也有很大差异.在Cu^2+掺杂的复合膜上，可以驱动共轭氧化还原反应，硫代乙醇酸/亚甲基蓝。由此可见，该膜起着电子传递膜的作用.由多肽和聚苯乙烯制备的膜具有相同的取向内部结构.测量了一些电解质通过GEL/PET和PBG-PGA/PS膜的渗透性;可以使用Stkes半径或考虑来自α-螺旋结构的聚集体来解释渗透性的顺序。在驱动共轭氧化还原反应穿过其上固定有酶的丝胶蛋白/PET膜的情况下，反应被加速，尽管酶活性非常低。这可以通过膜表面上的电子传递非常有效来解释。8.从上述事实可以得出结论，在电场存在下通过浇铸法制备的膜可以被认为是良好的细胞膜模型。