Synthesis of Mucus Glycoprotein in Ribosome of Rat Gastric Mucous Cell.

大鼠胃粘液细胞核糖体中粘液糖蛋白的合成。

• 批准号: 02807073
• 负责人: TSUKADA Hideaki
• 金额: $ 1.02万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807073/
• 关键词: Glycoprotein; Prostglandin; Glycosylation; Water-immersion Stress; Peptidyl-tRNA; PeptidylーtRNA; glycosylation; acylation; prostaglandin; ethanol; palmitic acid; galactosamine

Using gastric mucous cells which are involved exclusively in the synthesis of secretory O-glycosidic glycoprotein (mucin), the relationships between protein core synthesis and its acylation with fatty acids, O-glycosylation with N-acetylgalactosamine were investigated Labeling of the cells with[^3H]-proline, [^3H]-palmitic acid, [^<14>C]-galactosamine followed by isolation of peptidy -tRNA and release of nascent peptides indicated that the nascent peptide were already glycosylated and acylated in the ribosome of the rat gastric mucous cell.1. Prevention of alcohol-induced gastric mucosal injury by prostaglandin E-1 Effects of ethanol and prostaglandin on the glycoprotein synthesis in the gastric mucus cells have been evaluated. The rat gastric mucosal cells were subjected to a short-term tissue culture in the presence of ehtanol and prostaglandin El using[^3H]-proline, [^3H]-Palmitic acid, [^<14>C]-galactosamine. Low concentration of ehtanol (0.05-0.1M) stimulated the glycoprotein synt … More hesis, but higher concentration of ehtanol (0.5-1.5M) caused a marked reduction in the glycoprotein synthesis. Addition of prostaglandin resulted in the stabilization of the synthesis and secretion of the mucucs glycoprotein in the presence of ethanol.2. lnfluence of water-immersion stress on synthesis of mucus glycoprotein in rat gastric mucosa.Gastric raucous cells of rats subjected to water immersion stress were incubated with[^3H]-palmitic acid and[^<14>C]-N-acetylgalactosamine. The peptdyl-tRNA released by gastric polysomes was precipitated with cold ethanol and then the content was determined. A 70% reduction in the peptidyltRNA isolated was observed in the stress rats, as compared with in control rat. The peptide recoverd from the peptidyl-tRNA showed 30-50% less[3H]-Palmitic acid and[^<14>C]- N-acetylgalactosamine incorporation in the stressed rats than in normal control. It was thus suggested that translation, acylation and glycosylation of the peptides in the ribosomes of the gastric mucosa were remarkably affected by the stress. Less

使用专门参与分泌性O-糖苷糖蛋白（粘蛋白）合成的胃粘液细胞，研究了蛋白质核心合成与其脂肪酸酰化、N-乙酰半乳糖胺O-糖基化之间的关系。用[^3H]-脯氨酸、[^3H]-棕榈酸、[^3H]-棕榈酸、[^3H]-脯氨酸、[^3H]-棕榈酸、 [14C]-半乳糖胺随后分离肽-tRNA并释放新生肽表明新生肽已经在大鼠胃粘液细胞的核糖体中糖基化和酰化。1．前列腺素 E-1 预防酒精引起的胃粘膜损伤 已经评估了乙醇和前列腺素对胃粘液细胞中糖蛋白合成的影响。在乙醇和前列腺素E1存在下，使用[ 3 H]-脯氨酸、[ 3 H]-棕榈酸、[ 14 C]-半乳糖胺对大鼠胃粘膜细胞进行短期组织培养。低浓度的乙醇（0.05-0.1M）刺激糖蛋白合成，但较高浓度的乙醇（0.5-1.5M）导致糖蛋白合成显着减少。添加前列腺素可在乙醇存在下稳定粘液糖蛋白的合成和分泌。 2．水浸应激对大鼠胃粘膜粘液糖蛋白合成的影响。将经受水浸应激的大鼠胃沙哑细胞与[^ 3 H]-棕榈酸和[^ 14 C]-N-乙酰半乳糖胺一起孵育。胃多核糖体释放的肽基-tRNA用冷乙醇沉淀，然后测定含量。与对照大鼠相比，应激大鼠中分离出的肽基 tRNA 减少了 70%。从肽基-tRNA回收的肽显示，应激大鼠中的[ 3 H]-棕榈酸和[ 14 C]-N-乙酰半乳糖胺掺入比正常对照少30-50％。因此表明，胃粘膜核糖体中肽的翻译、酰化和糖基化受到应激的显着影响。较少的

项目成果

Tsukada H et al: "Prevention of Alcohol-induced Gastric Mucosal Injury by Porstaglandin E-1" Jap J Gastroenterolgy87. 10. 2342-2351 (1990)

Tsukada H 等人：“前列腺素 E-1 预防酒精引起的胃粘膜损伤”Jap J Gastroenterolgy87。

塚田 英昭・他: "Prostaglandin Eー1のエタノ-ル胃粘液合成傷害に及ぼす影響" 日本消化器病学会雑誌. 87(10). 2342-2351 (1990)

Hideaki Tsukada 等：“前列腺素 E-1 对乙醇胃粘液合成损伤的影响”日本胃肠病学会杂志 87(10) 2342-2351 (1990)。

塚田 英昭・他: "ProstaglandinEー1の消化管粘膜に与える影響" 日本実験潰瘍研究会誌. 18. 80-84 (1991)

Hideaki Tsukada 等：“前列腺素 E-1 对胃肠道粘膜的影响”日本实验溃疡研究会杂志 18. 80-84 (1991)。