肉視鏡的生検材料を用いたヒト小腸粘膜グルコース輸送の検討(グルコース吸収抑制物質の検討)

使用肉眼活检材料检查人小肠粘膜葡萄糖转运（葡萄糖吸收抑制剂检查）

• 批准号: 10670468
• 负责人: TSUKADA Hideaki
• 金额: $ 0.64万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670468/
• 关键词: Disaccharidase; Ginseng Radix; SGLT; Glucose transporter; GLUT2; Ussing's method; Triglitazone; Okadaic acid; 糖輸送担体; Triglitazone; グルコース輸送; 内視鏡生検

In this study NaィイD1+ィエD1-dependent glucose transport in human small intestine could be examined using endoscopic biopsy. Comparative study of inhibitory effect on glucose and maltose absorption elucidated that chemicals inhibits disaccharidase such as acarbose or inhibits sodium dependent glucose transporter such as Ginseng Radix (ref,1). Ginseng radix (GR) has been reported to inhibit hyperglycemia. This agent has been mainly used to treat diabetes in clinical practice. It has been reported that the action mechanism of GR involves enhancement of insulin biosynthesis and increased glucose transporter (GLUT-2) expression in the liver. When short-circuit current (Isc) in the human duodenal mucosa was measured before GR administration, Isc was 5.7±3.7uA/cm2 with sugar free Ringer's solution on both the mucosal and serosal sides. Addition of 10mM glucose or maltose to the mucosal side chamber increased Isc 2-10minutes after administration. Subsequently, GR administration inhibited the increase in Isc within 2 minutes in both the glucose-treated group and the maltose-treated group. These results suggests that Ginseng Radix ameliorates hyperglycemia by inhibiting NaィイD1+ィエD1-glucose co-transporter directly. Triglitazone inhibits electrogenic bicarbonate secretion in human small intestine (ref.2). Okadaic acid evokes an increase in water and electrolyte permeability of intestinal epithelium by opening the paracellular pathway (ref.3). Human gastric mucosa obtained by endoscopic biopsy is also useful for electrophysiological study (ref.4).

在这项研究中，可以使用内窥镜活检来检查人小肠中Na + D1 + D1依赖的葡萄糖转运。对葡萄糖和麦芽糖吸收抑制作用的比较研究阐明，化学物质抑制二糖酶（如阿卡波糖）或抑制钠依赖性葡萄糖转运蛋白（如人参）（参考文献，1）。人参根（GR）已被报道抑制高血糖症。在临床实践中，该药主要用于治疗糖尿病。据报道，GR的作用机制涉及促进胰岛素生物合成和增加肝脏中葡萄糖转运蛋白（GLUT-2）的表达。当在GR给药前测量人十二指肠粘膜中的短路电流（Isc）时，粘膜和浆膜侧的无糖林格氏液的Isc为5.7±3.7uA/cm 2。在粘膜侧室中加入10 mM葡萄糖或麦芽糖，在给药后2- 10分钟增加Isc。随后，GR给药在葡萄糖处理组和麦芽糖处理组中均在2分钟内抑制了Isc的增加。提示人参可能通过直接抑制Na + D1+葡萄糖转运蛋白来改善高血糖。Triglitazone抑制人小肠中的产电碳酸氢盐分泌（参考文献2）。冈田酸通过打开细胞旁通路引起肠上皮细胞水和电解质渗透性增加（参考文献3）。通过内窥镜活检获得的人胃粘膜也可用于电生理学研究（参考文献4）。

项目成果

Onomura, M., Tsukada, H., etal: "Effects of ginseng radix sugar absorption in the small."American Journal of Chinese Medicine. 27(Nos.3-4). 347-354 (1999)

Onomura，M.，Tsukada，H.，等人：“人参糖吸收的影响。”美国中医杂志。

Fukuda, K, Tsukada, H.: "Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa invitro."Peptides. 20. 127-132 (1999)

Fukuda, K, Tsukada, H.：“肾上腺髓质素在体外促进大鼠和人胃粘膜上皮的恢复。”肽。

Fukuda,K.,Tsukada,H.: "Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa in vitro"Peptides. 20. 127-132 (1999)

Fukuda，K.，Tsukada，H.：“肾上腺髓质素在体外促进大鼠和人胃粘膜上皮恢复”肽。

Fukuda,K., Tsukada,H.: "Adrenomedullin promotes epithelial restitutim of rat and human qastric mucosa in vitro"Peptides. 20. 127-132 (1999)

Fukuda，K.，Tsukada，H.：“肾上腺髓质素在体外促进大鼠和人胃粘膜上皮恢复”肽。

Fukuda K,et al.: "Effects of Adrenomedullin on Ion Transport and Muscle Contraction in Rat Distal Colon" Peptides. 19(6). 1043-1047 (1998)

Fukuda K 等人：“肾上腺髓质素对大鼠远端结肠离子运输和肌肉收缩的影响”肽。