Studies on pathogenesis of IgA nephropathy
IgA肾病发病机制的研究
基本信息
- 批准号:02807098
- 负责人:
- 金额:$ 0.9万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1991
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) Affinitiy to goat IgG of serum IgA from patients with IgA nephritisELISA showed that serum IgA from patients with IgA nephritis bound to goat IgG. Mean absorbance of ELISA in 32 IgA nephritis patients was segnificantly highir than that in normal human sera, but not difficant from that in other types of glomerulonephritis. IgA rheumaloid factor in plasma is not specific to IgA nephritis.2) Tissue-type plasminogen activator and its inhibitor in human glomerulonephrtis 1)We carried out an immunohistochemical study of tissue-type plasminogen activator (PA) and urokinase-type PA, and their inhibitors, PA inhibitor-1 and PA inhibitor-2, using renal biopsy specimens obtained from 86 patients with various forms of glomerulonephritis. The control were four normal renal tissue specimens.On immunofluorescent observation, granular staining for tissue-type PA was found to be distributed along the glomerular capillary walls. The fluorescence was weak in the normal renal tissue and occasionally intense in the tissues of patients with IgA nephritis, minimal change nephrotic syndrome, and lupus nephritis. PA inhibitor-1 was abundant in the glomerular epithelial cells and scarce in the mesangial area and glomerular capillary lumens of the normal renal tissues. This was confirmed by immunoelectron microscopy using gold staining.3) Transforming growth factor-beta protein and mRNA in glomeruli in normal and diseased human kidneys 2)Expression of transforming growth factor-beta1 (TGF-beta1) in normal and diseased human kidneys was examined by immunohistochemical staining with two antibodies, and by in situ hybridization with an oligonucleotide probe.The results indicate that mature TGF-beta and TGF-beta-LAP are localized in association with the matrix components of GBM or mesangium, and with immune deposit in human glomeruli. Expression of TGF-beta, mainly synthesized by mesangial cells, is enhanced in human glomerular diseases, and may contribute to the mesangial matrix increase.
1)伊加肾炎患者血清伊加与山羊IgG有亲和力,ELISA结果显示伊加肾炎患者血清伊加与山羊IgG结合。32例伊加肾炎患者的ELISA平均吸光度明显高于正常人血清,但与其他类型肾小球肾炎无显著差异。2)人肾小球肾炎组织型纤溶酶原激活物及其抑制物的研究1)对86例不同类型肾小球肾炎患者肾活检组织中的组织型纤溶酶原激活物(PA)、尿激酶型PA及其抑制物PA抑制物-1和PA抑制物-2进行了免疫组化研究。免疫荧光染色显示组织型PA颗粒分布于沿着肾小球毛细血管壁。正常肾组织荧光较弱,伊加肾炎、微小病变型肾病综合征和狼疮性肾炎患者肾组织中偶见强荧光。PA抑制剂-1在正常肾组织的肾小球上皮细胞中表达丰富,在系膜区和肾小球毛细血管腔内表达较少。这通过使用金染色的免疫电子显微镜证实。3)正常和患病的人肾的肾小球中的转化生长因子-β蛋白和mRNA 2)转化生长因子-β 1的表达用两种抗体通过免疫组织化学染色检测正常和患病人肾脏中的TGF-β 1,结果表明,成熟的TGF-β和TGF-β-TGF-β受体与GBM或系膜的基质成分相关,并在人肾小球内有免疫存款。在人类肾小球疾病中,主要由系膜细胞合成的TGF-β的表达增强,并且可能有助于系膜基质的增加。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aya N, Yoshioka K, Murakami K, Hino S, Okada M, Matsuo O, Maki S: "tissue-type plasminogen activator and its unhibitor in human glomerulonephritis." J Phthol.
Aya N、Yoshioka K、Murakami K、Hino S、Okada M、Matsuo O、Maki S:“人类肾小球肾炎中的组织型纤溶酶原激活剂及其非抑制剂。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
K Yoshioka,M Tohda,T Takemura,N Akano,K Matsubara,A Ooshima,S Maki: "Distribution of type I collagen in human kidney diseases in comparison with type III coIIagen." Jounal of Pathology. 162. 141-148 (1990)
K Yoshioka、M Tohda、T Takemura、N Akano、K Matsubara、A Ooshima、S Maki:“与 III 型胶原蛋白相比,I 型胶原蛋白在人类肾脏疾病中的分布。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshioka K, Takemura T, Murakami K, Aya N, Hino S, Miyazato H, Akano N, Maki S: "Transforming growth factor-bin normal and diseased human kidneys." Lab Invest.
Yoshioka K、Takemura T、Murakami K、Aya N、Hino S、Miyazato H、Akano N、Maki S:“转化生长因子箱正常和患病的人类肾脏。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshioka K,Akano N,Takemura T,Okada M,Maki S,Inai S,Akita H,Koitabashi Y,Takekoshi Y: "IgA nephropathy in patients with congenital C9 deficiency:Evidence for the role of membrane attack in human glomerular injury." Kidney International.
Yoshioka K、Akano N、Takemura T、Okada M、Maki S、Inai S、Akita H、Koitabashi Y、Takekoshi Y:“先天性 C9 缺乏症患者的 IgA 肾病:膜攻击在人类肾小球损伤中作用的证据。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Yoshioka K,Akano N,Takemura T,Okada M,Maki S,Inai S,Akita H,Koitabashi Y,Takekoshi Y: "IgA nephropathy in patients with congenital C9 deficiency:Evidence for the role of membrane attack complex in human glomerular injury." Kidney International.
Yoshioka K、Akano N、Takemura T、Okada M、Maki S、Inai S、Akita H、Koitabashi Y、Takekoshi Y:“先天性 C9 缺乏症患者的 IgA 肾病:膜攻击复合物在人类肾小球损伤中作用的证据。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
YOSHIOKA Kazuo其他文献
YOSHIOKA Kazuo的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('YOSHIOKA Kazuo', 18)}}的其他基金
Development of the high-resolution EUV detector for space plasma observation from spacecraft.
开发用于航天器空间等离子体观测的高分辨率 EUV 探测器。
- 批准号:
25871211 - 财政年份:2013
- 资助金额:
$ 0.9万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Molecular cloning,expression,and chromosomal localization of a human tubulointerstitial nephritis antigen
人肾小管间质性肾炎抗原的分子克隆、表达及染色体定位
- 批准号:
11470177 - 财政年份:1999
- 资助金额:
$ 0.9万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Hereditary nephritis (Alport syndorome) : abnormalities in type IV collagen alpha5 chain and skin diagnosis
遗传性肾炎(Alport 综合征):IV 型胶原 α5 链异常和皮肤诊断
- 批准号:
07670920 - 财政年份:1995
- 资助金额:
$ 0.9万 - 项目类别:
Grant-in-Aid for Scientific Research (C)