Research of Ovum Capture Inhibitor (OCI) in Endometriosis ; a Study of Anti-OCI Production, Exploitation of Immunological OCI Measurement, Localization and Production of OCI.

我们发现子宫内膜异位症患者腹腔液中含有丰富的卵子捕获抑制剂（ovum capture inhibitor，OCI）。OCI在金黄地鼠菌毛处形成膜（OCIRM：OCI相关膜），其在体外抑制卵母细胞-卵丘拾取。OCIRM由纤维状和碎屑状部分组成。我们设计了这些研究来找出OCI的表征。1）为了评估OCI分子量，研究了PF（Sephacryl S-300）的凝胶过滤。2）用抗纤维蛋白原（Fg）和抗PF（PF免疫兔血清）对OCIRM进行免疫染色<PF was filtrated over 1OOK Daltons with mi11ipore fi1ter>，并研究两种试剂的抗OCI活性。纤维部分被两者染色，但不像债务部分。3）用3种凝集素（Con-Al、WGA、SBA）进行免疫染色。用Con-A和WGA对OCIRM进行染色。4）研究了PF的OCI活性与总蛋白和纤维蛋白原浓度、PF与凝集素（Con-A、WGA、Lentil）的沉淀量（N=13）的关系。OCI活性与总蛋白和纤维蛋白原浓度，沉淀物量与除小扁豆外的凝集素之间没有关系。结论：OCI由200- 600 K道尔顿的大分子组成。OCIRM的纤维部分疑似纤维蛋白，但碎片样部分疑似凝集素结合糖缀合物。OCI活性与纤维蛋白原和凝集素结合物质一起出现，但纤维蛋白原不是必需的，主要因素是凝集素结合物质。

We have found out the OCI (ovum capture inhibitor) abundant in endometriosis peritoneal fluid (PF). OCI makes membranes (OCIRM : OCI related membranes) at Golden hamster fimbria which inhibit oocyte-cumulus pick up in vitro. OCIRM constitutes of fibrous and debris like part. We designed these studies to find out the characterization of OCI.1) To evaluate the OCI molecular weight gel filtration of PF (Sephacryl S-300) was studied. OCI activity was found at 200- 600K Daltons filtrates gatherings.2) Immuno-stain of OCIRM by anti-fibrinogen and anti-PF (immunized rabbit serum by PF<PF was filtrated over 1OOK Daltons with mi11ipore fi1ter>), and anti -OCI activity with both agents were studied. Fibrous part was stained by both but not debtis like part. OCI activity was not suppressed by both agent, but debtis like part was only made.3) With 3 Lectins (Con-Al WGA, SBA) immuno-stainings were studied. OCIRM were stained by Con-A and WGA.4) OCI activity of PF versus total protein and fibrinogen concentration, sediment amount of PF (PF with lectin makes sediments) with lectins (Con-A, WGA, Lentil) were studied (N=13). No relationships were noted between OCI activity and total protein and fibrinogen concentrations, sediment amount with lectins other than Lentil. Conclusions : OCI is constituted of plural large molecules (200- 600K Daltons). Fibrous part of OCIRM was fibrin suspected, but debris like part was lectin binding glycoconjugates suspect. OCI activity appears with fibrinogen and lectin binding substances, but fibrinogen is not necessary and major factor is lectin binding substances.