Role of Endocrine Systems on Changes of Kidney Function during Aging

衰老过程中内分泌系统对肾功能变化的作用

• 批准号: 02640581
• 负责人: KOBAYASHI Yuta
• 金额: $ 1.22万
• 依托单位: Shimane Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02640581/
• 关键词: Aging; Water-electrolyte metabolism; Kidney; Calcium Metabolism; Osteoporosis; Atrial Natriuretic peptide; Wister; Tw rats; NOD mice; アルドステロン; 血漿レニン活性; 大腿骨; カルシトニン; 性ホルモン; 水・電解質代謝; カルシウム; Wister; Tw; パラトルモン; 糖尿病マウス

Wistar/Tw strain male rats show polydipsia and polyuria after 16 months of age, while after 20 months of age in female rats. Current studies have suggested that this strain is a model for accelerated aging in water and electrolyte metabolism and kidney function, which are important for the control of homeostasis of animals. In the present study, age-related changes of kidney function and role of endocrine systems on the changes were investigated using this substrain rats.1. Kidney function during aging was studied and age-related increases of serum phospholipids and triglyceride were observed in female rats. These changes are considered to agree with the changes of serum parameters with dysfunction of kidney in human. 2. No significant change on serum atrial natriuretic peptide(ANP)concentration was observed during aging of this strain. Changes on ANP with the occurrence of diabetes mellitus were studied using non-obese diabetic(NOD)mice, as the diabetes mellitus animals also show poly … More dipsia. Significant reduction of serum ANP concentration and significant enhancement of atrial ANP content were observed. 3. Reduction of serum aldosterone concentration and plasma renin activity(1 to 10 fold)during aging of Wistar/Tw strain was observed. These results may be explained by the reduction of release of renin with the pathological changes of kidney. Increase of serum vasopressin concentration during aging has reported. Thus, changes of serum hormones which related to water and electrolyte metabolism have clarified in this substrain. 4. Furthermore, marked increase(10 fold)of urinary excretion of calcium in 24 hours with aging of female rats was found. 5. Reduction of bone materials in X-ray photograph, significant reductions of dry weight and calcium content in the femur, significant reductions of dry weight and mechanical strength of tail vertebrae were observed. These changes suggest that this female rats may be a model rat for spontaneous osteoporosis. 6. Serum calcitonin concentration showed no significant change.Further studies are necessary to clarify the mechanism of such changes in calcium metabolism during aging. Less

Wistar/Tw品系雄性大鼠在16月龄后出现多饮和多尿，而雌性大鼠在20月龄后出现多饮和多尿。目前的研究表明，该菌株是水电解质代谢和肾功能加速衰老的模型，这对于控制动物的体内平衡很重要。本研究以该亚系大鼠为研究对象，探讨了其与年龄相关的肾功能变化以及内分泌系统对其变化的影响。 1．研究了衰老过程中的肾功能，并观察到雌性大鼠血清磷脂和甘油三酯与年龄相关的增加。这些变化被认为与人体肾功能障碍时血清参数的变化一致。 2.该菌株老化过程中血清心房钠肽(ANP)浓度未见明显变化。使用非肥胖糖尿病 (NOD) 小鼠研究了 ANP 随糖尿病发生的变化，因为糖尿病动物也表现出多尿。观察到血清 ANP 浓度显着降低，心房 ANP 含量显着增加。 3.观察到Wistar/Tw品系衰老过程中血清醛固酮浓度和血浆肾素活性降低(1～10倍)。这些结果可能是由于肾脏病理变化导致肾素释放减少所致。据报道，衰老过程中血清加压素浓度增加。因此，在该亚种中与水和电解质代谢相关的血清激素的变化已得到阐明。 4.此外，随着雌性大鼠年龄的增长，24小时尿钙排泄量显着增加（10倍）。 5、X线照片骨材料减少，股骨干重和钙含量显着降低，尾椎干重和机械强度显着降低。这些变化表明这只雌性大鼠可能是自发性骨质疏松症的模型大鼠。 6.血清降钙素浓度无明显变化。衰老过程中钙代谢变化的机制需要进一步研究。较少的

项目成果

Shuichi Yano: "Atrial natriuretic peptide (ANP) levels in plasma and atrial auricles of the nonーobese diabetic (NOD) mouse In: Diabetic Heart" Kluwer Academic Publishers eds by M.Nagano and S.Mochizuki, 17

Shuichi Yano：“非肥胖糖尿病 (NOD) 小鼠血浆和心房心房中的心钠素 (ANP) 水平：糖尿病心脏”，M.Nagano 和 S.Mochizuki 编着的 Kluwer 学术出版社，17

Yano, Shuichi et al.: "Effect of diabetes mellitus on level of atrial natriuretic hormone in plasma and the right atrium in the non-obese diabetic mouse." Acta Endocrinol. (Copenh). 124. 595-601 (1991)

Yano、Shuichi 等人：“糖尿病对非肥胖糖尿病小鼠血浆和右心房心钠素水平的影响。”

Mifune, Hiroharu et al.: "Atrial natriuretic peptide (ANP) : a study of ANP and its mRNA in cardiocytes, and of plasma ANP levels in non-obese diabetic mice." Cell Tissue Res. 267. 267-272 (1992)

Mifune、Hiroharu 等人：“心房钠尿肽 (ANP)：对心肌细胞中 ANP 及其 mRNA 以及非肥胖糖尿病小鼠血浆 ANP 水平的研究。”

Shuichi Yano: "Effects of diabetes mellitus on levels of atrial natriuretic hormon (ANP) in plasma and the right atrium in the nonーobese diabetic (NOD) mouse" Acta Endocrinologica.

Shuichi Yano：“糖尿病对非肥胖糖尿病 (NOD) 小鼠血浆和右心房心钠素 (ANP) 水平的影响”《内分泌学报》。

Mifune,Hiroharu: "Atrial natriuretic peptide (ANP):a study of ANP and its mRNA in cardiocytes,and of plasma ANP levels in non-obese diabetic mice." Cell Tissue Res.267. 267-272 (1992)

Mifune、Hiroharu：“心房钠尿肽 (ANP)：对心肌细胞中 ANP 及其 mRNA 以及非肥胖糖尿病小鼠血浆 ANP 水平的研究。”