Physiological role of atrial natriuretic polypeptides on lactation

心房钠尿多肽对泌乳的生理作用

• 批准号: 15570049
• 负责人: KOBAYASHI Yuta
• 金额: $ 2.37万
• 依托单位: Shimane University (2004)Shimane Medical University (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15570049/
• 关键词: atrial natriuretic peptide(ANP); oxytocin; atrium; mammary gland; V-type ANP; mice; adrenomedullin; sympathetic nerve system; オキシトシン受容体; ANP受容体

Plasma A-type atrial natriuretic peptide(ANP) concentration after delivery in ICR mice was significantly higher than those during pregnancy and in virgin controls. It continued to increase until the 15th day of lactation (L15). On the day of delivery, ANP- immunoreactivity(IR) and the number of ANP-granules in the cardiocytes decreased compared to those during pregnancy and to those in virgin controls, and then they both began to increase continually until L15. The present results may suggest the significant role of ANP during lactation, suggesting active synthesis and release of ANP. On the day of delivery, Oxytocin(OX) is released. Thus, we get a hypothesis that OX may stimulate ANP release at the delivery and during the lactation and released ANP may affect mammary function through the receptors in the mammary gland. Concerning the evolution of ANP family peptides, eel V-type ANP (VNP) showed potent vasodilatory activity in canine coronary artery compared with human ANP or B-type ANP (BNP), suggesting a possible occurrence of this molecule in the mammals. During evolution, ANP family peptides may have a novel role in mammals as a control activity on mammary function in addition to their conservative role. In addition, vasodilatory response of a fish-origin peptide found recently from human, adrenomedullin 2 in porcine coronary artery was also studied. Further studies on the present hypothesis on pituitary-atrium-mammary gland axis are necessary especially from evolutional aspect.

ICR小鼠分娩后血浆A型心钠素（ANP）浓度显著高于妊娠期和处女对照组。持续增加至泌乳第15天（L15）。在分娩当天，心钠素免疫反应性（IR）和心钠素颗粒的数量在心肌细胞中的下降相比，在怀孕期间和那些在处女对照组，然后他们都开始不断增加，直到L15。提示ANP在哺乳期的重要作用，提示ANP的主动合成和释放。分娩当天，催产素（Oxytocin，OX）释放。因此，我们得出一个假设，即OX可能在分娩时和哺乳期间刺激ANP释放，并且释放的ANP可能通过乳腺中的受体影响乳腺功能。关于ANP家族肽的进化，与人ANP或B型ANP（BNP）相比，鳗鱼V型ANP（VNP）在犬冠状动脉中显示出强有力的血管舒张活性，表明该分子可能存在于哺乳动物中。在进化过程中，ANP家族肽在哺乳动物中除了其保守作用外，还可能具有控制乳腺功能的新作用。此外，还研究了最近从人类中发现的鱼源肽肾上腺髓质素2在猪冠状动脉中的血管扩张反应。对垂体-心房-乳腺轴假说的进一步研究，特别是从进化的角度，是有必要的。

项目成果

新規ヒトペプチド;アドレノメデュリン2の冠血管拡張作用

新型人肽；肾上腺髓质素2的冠状血管扩张作用

• 发表时间: 2004
• 期刊: 日本薬理学雑誌 124(捕冊1)
• 作者: 小林裕太

李頌華, 王如偉, 田丸直美, 都仁哉, 岩崎純夫, 小林裕太, 奥西秀樹: "アトピー性皮膚炎94症例における新中医薬処方「複方苦参」の治療効果"炎症・再生. 24・1. 55-59 (2004)

李旭华、王如伟、Naomi Tamaru、Jinya Miyako、Sumio Iwasaki、Yuta Kobayashi、Hideki Okunishi：“新中药方‘复方止痛人参’对特应性皮炎94例的治疗效果”炎症/再生24・。 1. 55 -59 (2004)

CORONARY VASODILATORY RESPONSE TO A NOVEL PEPTIDE, ADRENOMEDULLIN 2

• DOI: 10.1111/j.1440-1681.2004.04115.x
• 发表时间: 2004-12
• 期刊: Clinical and Experimental Pharmacology and Physiology
• 影响因子: 2.9
• 作者: Yuta Kobayashi;Yingjie Liu;T. Gonda;Y. Takei

小林裕太: "アミノ酪酸-血液・尿及び脳脊髄液試料"広範囲血液・尿化学検査、免疫学的検査-その数値をどう読むか 5-1 日本臨床(増刊). 62(増刊)(印刷中). (2004)

小林裕太：《氨基丁酸-血液、尿液和脑脊液样本》广泛的血液和尿液化学检查、免疫学检查-如何读取数字5-1日本临床（特刊）62（特刊）（打印）。 (2004)

Four natriuretic peptides (ANP, BNP, VNP and CNP) coexist in the sturgeon: identification of BNP in fish lineage

• DOI: 10.1677/jme.0.0320547
• 发表时间: 2004-04-01
• 期刊: JOURNAL OF MOLECULAR ENDOCRINOLOGY
• 影响因子: 3.5
• 作者: Kawakoshi, A;Hyodo, S;Takei, Y
• 通讯作者: Takei, Y