Generalization of nonisotopic DNA fingerprinting

由亚历克斯·杰弗里斯开发的DNA指纹技术被用来识别个体。这种方法将染色体的小卫星树脂可视化为高度多态的条带。我们利用ECL(增强化学发光)技术建立了一种简单的非同位素DNA指纹分析方法。这种方法具有与常规放射程序相当的分辨率和灵敏度。这项研究的目的是允许传统实验室在不使用放射性同位素的情况下进行常规的DNA指纹分析。根据我们的方案，在11个法律医学实验室进行了DNA指纹分析。用HAeIII酶切三个人的DNA，并用过氧化物酶标记的小卫星“myo”进行探测，然后比较在每个实验室获得的三个人的DNA的带型。所有调查人员都能在X光片上发现清晰的条带。这些DNA指纹模式并不完全相同，但非常相似。这些数据表明，很难知道单独胶片上的样品是否精确匹配。然而，通过一次Southern印迹的DNA条带检测，这种冷法可以应用于亲子鉴定和合子诊断。

DNA fingerprinting, developed by Alex Jeffreys, is used to identify individuals. This method visualizes minisatellite resins of chromosomes as highly polymorphic bands. We established a simple nonisotopic method for DNA fingerprinting using ECL (enhanced chemiluminecent) technique. This method has comparable resolution and sensitivity to regular radioactive procedures.The aim of this study was to allow conventional laboratories to routinely perform DNA fingerprinting analysis without the use of radioisotopes. DNA fingerprinting according to our protocol was carried out in 11 laboratories of legal medicine. Human DNAs from three individuals were digested with HaeIII and probed with peroxidase-labelled minisatellite "myo" Then the band patterns of the three obtained in each laboratory were compared. All of the investigators could detect clear bands on X-ray film. Those patterns of DNA fingerprint were not identical but very similar. These data revealed that it is difficult to know exact matching of samples on separate films. However, by examination of the DNA bands on one Southern blot this cold method could be applied to paternity testing and zygosity diagnosis.