Generalization of nonisotopic DNA fingerprinting

由Alex Jeffreys开发的DNA指纹识别用于识别个人。该方法将染色体的小卫星树脂可视化为高度多态性条带。建立了一种简单的非同位素ECL DNA指纹分析方法。这种方法具有可比的分辨率和灵敏度，定期放射性procedure.The目的，这项研究是让传统的实验室进行DNA指纹分析，而不使用放射性同位素。根据我们的协议进行了DNA指纹在11个实验室的法律的医学。用Hae Ⅲ消化三个人的DNA，用过氧化物酶标记的小卫星“myo”探针探测，比较三个实验室的带型。所有研究者均能在X线片上发现清晰的条带。这些DNA指纹图案并不完全相同，但非常相似。这些数据表明，很难知道样品在单独的膜上的精确匹配。然而，通过对一个Southern印迹上的DNA条带的检查，这种冷方法可以应用于亲子鉴定和接合性诊断。

DNA fingerprinting, developed by Alex Jeffreys, is used to identify individuals. This method visualizes minisatellite resins of chromosomes as highly polymorphic bands. We established a simple nonisotopic method for DNA fingerprinting using ECL (enhanced chemiluminecent) technique. This method has comparable resolution and sensitivity to regular radioactive procedures.The aim of this study was to allow conventional laboratories to routinely perform DNA fingerprinting analysis without the use of radioisotopes. DNA fingerprinting according to our protocol was carried out in 11 laboratories of legal medicine. Human DNAs from three individuals were digested with HaeIII and probed with peroxidase-labelled minisatellite "myo" Then the band patterns of the three obtained in each laboratory were compared. All of the investigators could detect clear bands on X-ray film. Those patterns of DNA fingerprint were not identical but very similar. These data revealed that it is difficult to know exact matching of samples on separate films. However, by examination of the DNA bands on one Southern blot this cold method could be applied to paternity testing and zygosity diagnosis.