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Evaluation of tetrameric microsatellites for use in personal identification of Japanese

四聚体微卫星用于日本人身份识别的评估

基本信息

批准号：
07670484
负责人：
YAMANOUCHI Haruo
金额：
$ 1.47万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670484/
关键词：
Microsatellite STR Personal Identification PCR-based DNA typing vWF Haplotype AR Androgen recepter

项目摘要

Polymorphic microsatellites (short tandem repeat : STR) are the genetic markers very useful in human identification. We previously reported that PCR-based DNA typing of two tetrameric STRs vWF-p and TH01 provided an accurate and rapid discrimination for personal identification of Japanese. In this study, two STR loci vWF-k (TCTA) n and AR (CAG) n (CAA) were evaluated.(1) Typing for vWF-k of 460 unrelated Japanese was done. Nice alleles were observed. The heterozygosity was 0.798 and the polymorphism information content (PIC) was 0.765.(2) The usefulness of a haplotype analysis of vWF-k and vWF-p loci in forensic investigations was examined, since a haplotype produces more variation than an allotype. These two tetrameric repeat loci are located within approximately 700 bp of intron 40 of the von Willebrand factor gene. Their polymorphisms were determined by PCR for DNA obtained from a total of 57 families and haplotypes were constructed on the basis of the family data. As many as 29 haplotypes were identified of 116 unrelated individuals included in the families. Its heterozygosity and polymorphism information content were 0.984 and 0.928, respectively. Among the 116 individuals, 72 were distinguished using the simply combined genotyping of vWF-p. On the other hand, 77 diplotypes were determined with the haplotype analysis. This suggests usefulness of the testing in personal discrimination, especially in cases haveing the family information. Moreover, this haplotype analysis has proved to be a powerful tool in forensic case studies.(3) Typing for AR locus of 110 unrelated Japanese is under investigation.

多态性微卫星（STR）是一种在人类身份识别中非常有用的遗传标记。我们以前报道，基于PCR的DNA分型的两个四聚体STR vWF-p和TH 01提供了一个准确和快速的个人身份识别的日本人。本研究对两个STR基因座vWF-k（TCTA）n和AR（CAG）n（CAA）进行了检测。(1)对460名无关的日本人进行vWF-k分型。观察到良好的等位基因。杂合度为0.798，多态信息含量（PIC）为0.765。(2)检验了vWF-k和vWF-p基因座的单倍型分析在法医学调查中的有用性，因为单倍型比同种异型产生更多的变异。这两个四聚体重复位点位于血管性血友病因子基因内含子40的约700 bp内。对57个家系的DNA进行PCR检测，并根据家系资料构建单倍型。在116个家系中鉴定出29种单倍型。其杂合度和多态信息含量分别为0.984和0.928。在这116个个体中，vWF-p的简单联合基因分型可区分出72个个体，单倍型分析可区分出77个双倍型。这表明测试在个人歧视方面是有用的，特别是在有家庭信息的情况下。此外，这种单倍型分析已被证明是一个强大的工具，在法医案例研究。(3)110名无关日本人的AR基因座分型正在调查中。