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Development of fine fiberscope system for fluorescence observation of epidural and subarachnoid spaces

开发用于硬膜外和蛛网膜下腔荧光观察的细纤维镜系统

基本信息

批准号：
02557058
负责人：
SHIMOJI Koki
金额：
$ 4.61万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

Fine fiberscope systems for fluorescence observation of epidural and subarachnoid spaces were developed, and applications of the systems for monitoring of tissue oxygenation were investigated. A fiberscope system that was applied to fluorescence imaging is consisted of a Xe-lamp as a light source, a quartz fiber assembly (outer diameter:1.0mm)for irradiation of ultra-violet light, a fine endoscope, highly sensitive SIT camera and a video-imaging system. Although autofluorescence of the rat brain induced by irradiation of ultra-violet light (350nm) was week, it was clearly observed with this system. The brain tissue and the surface vessels were distinguishable in the endoscopic image of autofluorescence. Another fiberscope system is used for fluorescence spectrometry, and it is consisted of a laser-generator, a quartz fiber assembly (outer diameter:1.0mm) for irradiation of ultra-violet light and acquisition of fluorescence and a multichannel optical photodiode-array spectrometer. Autofluorescence spectra evoked by pulsed laser ultra-violet light (wavelength:363nm) were measured from the rat brain, and their changes induced by brain ischemia were observed. Two broad bands with peaks at 400nm and 460nm were observed in the autofluorescence spectra of the brain. The fluorescence with a peak at 460nm increased during brain ischemia induced by occlusion of the bilateral common carotid arteries under hypotension, while the peak at 400nm remained unchanged. The augmantation of autofluorescence during brain ischemia may be due to an increase in NAD(P)H under the hypoxic condition. These results suggest that the fine fiberscope systems for fluorescence observation will be applicable to clinical monitoring system for tissue oxygenation of the epidural and subarachnoid spaces.

研制了用于硬膜外和蛛网膜下腔荧光观察的精细纤维内窥镜系统，并对该系统在组织氧合监测中的应用进行了研究。一种用于荧光成像的纤维内窥镜系统，由氙灯作为光源、用于紫外光照射的石英光纤组件(外径为1.0 mm)、精细内窥镜、高灵敏度的SIT摄像机和视频成像系统组成。虽然350 nm紫外光照射大鼠脑内的自发荧光很弱，但该系统能清楚地观察到这种现象。在内窥镜下的自体荧光图像中可分辨出脑组织和表面血管。另一套用于荧光光谱分析的光纤系统，由激光发生器、用于紫外光照射和荧光采集的石英光纤组件(外径为1.0 mm)和多通道光电二极管阵列光谱仪组成。用脉冲激光紫外光(波长：363 nm)激发大鼠脑组织，测定其自发荧光光谱，并观察其在脑缺血后的变化。在脑的自体荧光光谱中观察到两个峰在400 nm和460 nm的宽带。在低血压下阻断双侧颈总动脉所致脑缺血时，460 nm处的荧光增强，而400 nm处的荧光强度不变。脑缺血时自发荧光的增强可能是由于缺氧条件下NAD(P)H的增加所致。这些结果表明，精细的纤维内窥镜荧光观察系统可用于硬膜外和蛛网膜下腔组织氧合的临床监测系统。