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A Control System for a Four-Sector Tandem Mass Spectrometer

四扇区串联质谱仪的控制系统

基本信息

批准号：
02558016
负责人：
SHIMONISHI Yasutsugu
金额：
$ 4.61万
依托单位：
Institute for Protein Research, Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02558016/
关键词：
Four-sector Tandem mass spectrometer CPU-controlled MS MS Acquisition Processor Unit Digital Analog Converters Multi-precursor lons Fragment Ions Stable-isotope Labeling of peptides Peptide And Protein Sequencing 安定同位体 ^<18>O標識化タンデム質量

项目摘要

We have developed a new acquisition processor unit simultaneously to control MS1 and MS2 of a four-sector tandem mass spectrometer that makes it possible to acquire the MS/MS spectra from multi-precursor ions in a single measurement. The difficulty in operation of the present four-sector tandem mass spectrometer (MS1 needs to be manually switched mass to mass to the center of each precursor ion, resulting in time and sample loss) can be overcome by connecting it to the above control system.In the present system, to switch the magnetic field to the precise center of the precursor ions in a MS1, it can be correctly compensated by re-calibration using an appropriate calibrant interpolating each selected mass. Each precursor ion automatically selected in the MS1 can be decomposed independently by bombardment with helium in a collision cell, and resultant fragment ions derived from each precursor ion can be readily analyzed in a MS2. In this process, four sets of digital analo, converters (DAC) controlling magnetic and electric fields of both MSI and MS2 can be syiicliroiiized by system engineering work station (a data acquisition system in JMS-HX221CP, Institute for Protein Research).A four-sector tandem mass spectrometer (JMS-HX221CP) equipped with the above MS/MS control system was used for analyzing, primary structures of peptides and proteins. The CPUcontrolled MS/MS of multi-precursor ions was found to be effective and rapid in peptide and protein sequencing. F Urthermore, a combined use of a stable-isotope labeling, method of peptides and the present tandem mass spectrometer allowed easily and reliably to determine amino acid sequences of peptides and proteins.

我们已经开发了一种新的采集处理器单元，同时控制MS 1和MS 2的四扇区串联质谱仪，使它有可能获得MS/MS光谱从多个前体离子在一个单一的测量。现有四扇区串联质谱计的操作难点（MS 1需要手动地将质量切换到每个前体离子的中心，导致时间和样品损失）可以通过将其连接到上述控制系统来克服。在本系统中，为了将磁场切换到MS 1中前体离子的精确中心，可以通过使用适当的校准物内插每个选定的质量进行重新校准来正确地补偿。在MS 1中自动选择的每个前体离子可以通过在碰撞室中用氦轰击而独立地分解，并且可以在MS 2中容易地分析从每个前体离子衍生的所得碎片离子。在此过程中，利用系统工程工作站（蛋白质研究所JMS-HX 221 CP的数据采集系统）对MS 1和MS 2的4套控制磁场和电场的数字模拟转换器（DAC）进行了系统化，并利用配备了上述MS/MS控制系统的四扇区串联质谱仪（JMS-HX 221 CP）对多肽和蛋白质的一级结构进行了分析。多前体离子的CPU控制的MS/MS被发现是有效和快速的肽和蛋白质测序。F Urgent，一个稳定的同位素标记，肽的方法和本串联质谱仪的组合使用允许容易和可靠地确定肽和蛋白质的氨基酸序列。